295份国内外小麦种质资源条锈病抗性评价及抗病基因分子检测

doi:10.3724/SP.J.1006.2025.51048

Abstract

Abstract:

Highly virulent races of stripe rust pose a serious threat to global wheat production. In this study, we evaluated the resistance of 295 domestic and international wheat varieties (lines) to the currently prevalent races and pathogenic groups of Puccinia striiformis f. sp. tritici at both the seedling and adult plant stages. We also analyzed the presence of known stripe rust resistance genes to provide a foundation for breeding durably resistant varieties and for the effective utilization of resistant germplasm. Seedling-stage resistance was assessed under greenhouse conditions using the prevalent races CYR32 and CYR34. Adult-plant resistance was evaluated in the field during 2023–2024 at two locations—Lugang (Xinjiang) and Qingshui (Gansu)—using a composite inoculum comprising the prevalent races (CYR32, CYR33, and CYR34), the Shuiyuan pathogenic group (Su11-4 and Su11-5), and the Guinong 22 pathogenic group (G22-14). Molecular screening was performed using closely linked flanking or functional markers for 13 known resistance genes: Yr9, Yr15, Yr17, Yr18, Yr28, Yr29, Yr30, Yr80, Yr81, Yr82, Yr86, YrZH22, and YrZH84. Resistance identification results showed that 72 varieties (lines) were resistant to CYR32 and CYR34 at the seedling stage. Four varieties (Fr03733, Aikang 58, Jimai 22, and Qinnong 151) displayed immune responses to both races. A total of 154 accessions showed adult-plant resistance across both field environments, with 43 accessions exhibiting consistently high levels of resistance (HR). Thirteen accessions demonstrated resistance to both CYR32 and CYR34 at the seedling stage and high adult-plant resistance to the composite pathogen mixture (CYR32, CYR33, CYR34, Su11-4, Su11-5, and G22-14). Molecular detection revealed that 11 varieties (lines) carried four resistance genes, 39 carried three, 82 carried two, 115 carried one, and 48 varieties (lines) did not carry any of the tested genes. It is speculated that these varieties may possess other known or novel stripe rust resistance genes. The combination of different Yr genes and stable, highly resistant varieties (lines) identified in this study provides valuable resources for future wheat breeding programs targeting durable stripe rust resistance.

Key words: domestic and international wheat varieties (lines), wheat stripe rust, disease resistance evaluation, Yr genes, molecular detection

Jiao Wen-Juan, Bai Bin, Xiekelai Yilamu, Zhang Fei-Fei, Jia Qiu-Zhen, Geng Hong-Wei, Cheng Yu-Kun. Evaluation of stripe rust resistance in 295 domestic and foreign wheat germplasm resources and molecular detection of resistance genes[J].Acta Agronomica Sinica, 0, (): 0-.

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