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Identification of RAV family in 25 different plant species and expression analysis of RAV genes in potato

LI Wan1,*,CHANG Zi-Rui1,LU Yao2,SHEN Ri-Min2,ZHAO Yong-Ping1,*,BAI Xiao-Dong2,*   

  1. 1 School of Biomedicine and Food Engineering, Shangluo University, Shangluo 726000, Shaanxi, China; 2 Key Laboratory of Potato Genetic Improvement and Germplasm Innovation in Shanxi Province, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
  • Received:2025-05-17 Revised:2025-08-13 Accepted:2025-08-13 Published:2025-08-22
  • Supported by:
    This study was supported by the Natural Science Basic Research Program of Shaanxi (2025JC-YBMS-245) and the Key Laboratory of Potato Genetic Improvement and Germplasm Innovation in Shanxi Province, Shanxi Agricultural University (202304010921003-K06).

Abstract:

Plants are susceptible to various environmental factors such as extreme temperatures, high salinityand drought, which can hinder their development and significantly reduce crop yields. The RAV (Related to ABI3/VP1) gene family is unique to plants and is closely associated with growth, developmentand responses to both biotic and abiotic stresses. In this study, RAV genes/proteins (RAVs) from 25 plant species, ranging from Chlorophyta to Angiosperms, were identified and analyzed using bioinformatics tools and multiple databases. The results revealed that RAVs are more abundant in Gymnosperms and Angiosperms, present in smaller numbers in Bryophytes and Lycophytes, and absent in Chlorophyta. Phylogenetic analysis classified the RAVs into three main clades: Clade I included RAVs from Bryophytes to Angiosperms (excluding Monocots); Clade II contained only RAVs from EudicotsClade III comprised RAVs from Monocots and Marchantia polymorpha. Further analysis of RAVs in potato showed that although the number of RAV family members was limited, they exhibited significant functional divergence and played important roles in responses to abiotic stress. Overall, this study provides a comprehensive identification and characterization of RAVs across 25 plant species, offering a valuable reference for future functional studies of the RAV gene family.

Key words: RAV family, transcription factors, bioinformatics, Solanum tuberosum, abiotic stresses

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