Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica

   

Evaluation of stripe rust resistance and resistance genes in 81 major wheat cultivars from Gansu province, China

Zhang Bo1,*,Huang Jin1,Sun Zhen-Yu1,Cao Shi-Qin1,Li Pei-Ling2,Li Qing-Qing1,Guo Zhi-Jie1,*,Wang Wan-Jun3   

  1. 1 Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China; 2 Yuzhong Alpine Agricultural Experiment Station, Gansu Academy of Agricultural Sciences, Lanzhou 730010, Gansu, China; 3 Tianshui Agricultural Science Research Institute, Tianshui 741200, Gansu, China
  • Received:2026-04-20 Revised:2026-08-21 Accepted:2026-08-21 Published:2026-08-27
  • Supported by:
    This study was supported by the Key Research and Development Program of Gansu Academy of Agricultural Sciences (2026GAAS20), the National Key Research and Development Program (2021YFD1401000) and the National Natural Science Foundation of China (32060596).

Abstract:

To understand the resistance of wheat cultivars grown in Gansu to wheat stripe rust and the stripe rust resistance genes they carry, this study evaluated 81 winter wheat cultivars from Gansu for resistance to wheat stripe rust at all growth stages and detected stripe rust resistance genes. The seedling-stage resistance evaluation results showed that 1.23% of the wheat cultivars were highly resistant to wheat stripe rust, 4.94% were moderately resistant, and 93.83% were moderately susceptible. The adult-plant resistance evaluation results from 2023 to 2024 indicated that 11.11% of the wheat cultivars were immune to wheat stripe rust, 8.64% were highly resistant, 8.64% were moderately resistant, 39.51% exhibited slow rusting, and 32.10% were moderately susceptible. The adult-plant evaluation results from 2024 to 2025 revealed that 7.41% of the wheat cultivars were immune to stripe rust, 7.41% were highly resistant, 38.27% were moderately resistant, 17.28% exhibited slow rusting, and 29.63% were moderately susceptible. The disease resistance gene detection results showed that the numbers of cultivars carrying the resistance genes Yr26/Yr24, Yr29/Lr46/Pm39/Sr58, Yr30/Lr27/Pm70/Sr2, Yr75, Yr78, Yr80, Yr82, YrSP/Yr5, Lr34/Yr18/Pm38/Sr57, Lr67/Yr46/Pm46/Sr55, and QYrsn.nwafu-2AS/Yr17 were 9, 77, 47, 18, 41, 11, 11, 3, 10, 81, and 17, respectively. All 81 wheat cultivars carried two or more resistance genes. The study indicated that the majority of wheat cultivars currently grown in Gansu were moderately susceptible at the seedling stage. At the adult-plant stage, they exhibited varying degrees of resistance to stripe rust, although some remained moderately susceptible. These cultivars carried a rich array of adult-plant resistance genes but lacked genes conferring resistance at all growth stages. Only effective pyramiding and interaction of resistance genes can enhance disease resistance in wheat.

Key words: wheat cultivars, wheat stripe rust, disease resistance evaluation, resistance to stripe rust, disease resistance genes

[1] 刘万才, 王保通, 赵中华, 等. 我国小麦条锈病历次大流行的历史回顾与对策建议. 中国植保导刊, 2022, 42(6): 21–27.
Liu W C, Wang B T, Zhao Z H, et al. Historical review and countermeasures of wheat stripe rust epidemics in China. China Plant Prot, 2022, 42(6): 21–27 (in Chinese with English abstract).

[2] 陈万权, 刘太国. 我国小麦秋苗条锈病发生规律及其区间菌源传播关系. 植物保护, 2023, 49(5): 50–70.

Chen W Q, Liu T G. Occurrence of wheat stripe rust in autumn-sown seedlings and inter-regional inoculum dispersal of Puccinia striiformis f. sp. tritici in China. Plant Prot, 2023, 49(5): 50–70 (in Chinese with English abstract). 

[3] 杨作民, 唐伯让, 沈克全, 等. 小麦抗病育种的战略问题: 小麦对锈病、白粉病第二线抗源的建立和应用. 作物学报, 1994, 20: 385–394.
Yang Z M, Tang B R, Shen K Q, et al. A strategic problem in wheat resistance breeding: building and utilization of sources of second-line resistance against rusts and mildew in China. Acta Agron Sin, 1994, 20: 385–394 (in Chinese with English abstract).

[4] 杨作民, 解超杰, 孙其信. 后条中32时期我国小麦条锈抗源之现状. 作物学报, 2003, 29: 161–168.
Yang Z M, Xie C J, Sun Q X. Situation of the sources of stripe rust resistance of wheat in the post-CY32 era in China. Acta Agron Sin, 2003, 29: 161–168 (in Chinese with English abstract).

[5] 张勃, 孙振宇, 黄瑾, 等. 2008–2023年甘肃省中南部小麦条锈菌动态分布与生理小种变化. 植物保护学报, 2025, 52: 1363–1378.
Zhang B, Sun Z Y, Huang J, et al. Dynamic distribution and physiological race variation of wheat stripe rust Puccinia striiformis f. sp. tritici in south-central Gansu province from 2008 to 2023. J Plant Prot, 2025, 52: 1363–1378 (in Chinese with English abstract).

[6] 姚强, 王洁荣, 孟岩, 等. 中国小麦条锈病菌CYR32和CYR33的毒性及基因型多样性. 植物保护学报, 2018, 45: 46–52.

Yao Q, Wang J R, Meng Y, et al. Virulence and genotypic diversity of wheat stripe rust races CYR32 and CYR33 in China. J Plant Prot, 2018, 45: 46–52 (in Chinese with English abstract).

[7] Wan A M, Zhao Z H, Chen X M, et al. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Dis, 2004, 88: 896–904.

[8] Chen W Q, Wu L R, Liu T G, et al. Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis, 2009, 93: 1093–1101.

[9] 曹世勤, 王晓明, 贾秋珍, 等. 2003–2013年小麦品种(系)抗条锈性鉴定及评价. 植物遗传资源学报, 2017, 18: 253–260.
Cao S Q, Wang X M, Jia Q Z, et al. Evaluation of resistance to stripe rust in wheat varieties (lines) during 2003–2013 in Longnan region, Gansu province. J Plant Genet Resour, 2017, 18: 253–260 (in Chinese with English abstract).

[10] 中华人民共和国国家市场监督管理总局, 中国国家标准化管理委员会. 小麦抗病虫性评价技术规程:GB/T 45211.1-2025. 北京: 中国国家标准出版社, 2025.
State Administration for Market Regulation of the People’s Republic of China, China National Standardization Administration. Technical code of practice for evaluation of resistance to diseases and insect pests in wheat: GB/T 45211.1-2025. Beijing: China National Standards Press, 2025 (in Chinese).

[11] Xiang M J, Liu S J, Wang X T, et al. Development of breeder chip for gene detection and molecular-assisted selection by target sequencing in wheat. Mol Breed, 2023, 43: 13.

[12] Mcintosh R A. Catalogue of gene symbols for wheat: 2024 edition (covering all WGC curation) word files. Pathogenic disease/pest reaction, 2024, 84–102.

[13] McIntosh R, Mu J M, Han D J, et al. Wheat stripe rust resistance gene Yr24/Yr26: a retrospective review. Crop J, 2018, 6: 321–329.

[14] Jighly A, Alagu M, Makdis F, et al. Genomic regions conferring resistance to multiple fungal pathogens in synthetic hexaploid wheat. Mol Breed, 2016, 36: 127.

[15] Xiang M J, Tian B, Cao J H, et al. Yr29 combined with QYr.nwafu-4BL.3 confers durable resistance to stripe rust in wheat cultivar Jing 411. Theor Appl Genet, 2024, 137: 252.

[16] Wang X T, Xiang M J, Li H Z, et al. High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat. Theor Appl Genet, 2024, 137: 152.

[17] Pakeerathan K, Bariana H, Qureshi N, et al. Identification of a new source of stripe rust resistance Yr82 in wheat. Theor Appl Genet, 2019, 132: 3169–3176.

[18] Marchal C, Zhang J P, Zhang P, et al. BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust. Nat Plants, 2018, 4: 662–668.

[19] Kolmer J A, Singh R P, Garvin D F, et al. Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci, 2008, 48: 1841–1852.

[20] Herrera-Foessel S A, Singh R P, Lillemo M, et al. Lr67/Yr46 confers adult plant resistance to stem rust and powdery mildew in wheat. Theor Appl Genet, 2014, 127: 781–789.

[21] Huang S, Liu S J, Zhang Y B, et al. Genome-wide wheat 55K SNP-based mapping of stripe rust resistance loci in wheat cultivar shaannong 33 and their alleles frequencies in current Chinese wheat cultivars and breeding lines. Plant Dis, 2021, 105: 1048–1056.

[22] Coriton O, Jahier J, Leconte M, et al. Double dose efficiency of the yellow rust resistance gene Yr17 in bread wheat lines. Plant Breed, 2020, 139: 263–271.

[23] 陈建雄, 严浩浩, 曹世勤, 等. 62份甘肃小麦品种(系)抗条锈性评价及抗病基因检测. 植物病理学报, 2023, 53: 881–889.
Chen J X, Yan H H, Cao S Q, et al. Evaluation of resistance and detection of resistance gene(s) to stripe rust of 62 wheat cultivars (lines) in Gansu province. Acta Phytopathologica Sin, 2023, 53: 881–889 (in Chinese with English abstract).

[24] 李宇翔, 胡小平. 中国小麦条锈病传播路径研究进展. 植物病理学报, 2025, 55: 911–921.
Li Y X, Hu X P. Research progress on the migration pathways of wheat stripe rust in China. Acta Phytopathologica Sin, 2025, 55: 911–921 (in Chinese with English abstract).

[25] 刘博, 刘太国, 章振羽, 等. 中国小麦条锈菌条中34号的发现及其致病特性. 植物病理学报, 2017, 47: 681–687.
Liu B, Liu T G, Zhang Z Y, et al. Discovery and pathogenicity of CYR34, a new race of Puccinia striiformis f. sp. Tritici in China. Acta Phytopathologica Sin, 2017, 47: 681–687 (in Chinese with English abstract).

[26] 黄亮, 刘太国, 肖星芷, 等. 中国79个小麦品种(系)抗条锈病评价及基因分子检测. 中国农业科学, 2017, 50: 3122–3134.
Huang L, Liu T G, Xiao X Z, et al. Evaluation of stripe rust resistance and molecular detection of yr genes of 79 wheat varieties (lines) in China. Sci Agric Sin, 2017, 50: 3122–3134 (in Chinese with English abstract).

[27] 白斌, 张怀志, 杜久元, 等. 西北条锈菌源区冬小麦育种抗条锈病基因的利用现状与策略. 中国农业科学, 2024, 57: 4–17.
Bai B, Zhang H Z, Du J Y, et al. Current situation and strategy of stripe rust resistance genes untilization in winter wheat cultivars of northwestern oversummering region for Puccinia striiformis f. sp. tritici in China. Sci Agric Sin, 2024, 57: 4–17 (in Chinese with English abstract).

[28] Bariana H S, McIntosh R A. Characterisation and origin of rust and powdery mildew resistance genes in VPM1 wheat. Euphytica, 1994, 76: 53–61.

[29] 刘志勇, 张怀志, 白斌, 等. 中国小麦抗条锈病基因育种利用现状与策略. 中国农业科学, 2024, 57: 34–51.
Liu Z Y, Zhang H Z, Bai B, et al. Current status and strategies for utilization of stripe rust resistance genes in wheat breeding program of China. Sci Agric Sin, 2024, 57: 34–51 (in Chinese with English abstract).

[30] 焦文娟, 何万龙, 耿洪伟, 等. 155份春小麦品种(系)条锈病抗性评价与抗病基因分子检测. 中国农业科学, 2026, 59: 937–950.
Jiao W J, He W L, Geng H W, et al. Stripe rust resistance evaluation and molecular characterization of yr genes for 155 spring wheat varieties (lines). Sci Agric Sin, 2026, 59: 937–950 (in Chinese with English abstract).

[31] 习玲, 王昱琦, 杨修, 等. 243份云南普通小麦地方品种抗条锈病鉴定及分子标记检测. 中国农业科学, 2021, 54: 684–695.
Xi L, Wang Y Q, Yang X, et al. Evaluation of resistance to stripe rust and molecular detection of resistance gene(s) in 243 common wheat landraces from the Yunnan province. Sci Agric Sin, 2021, 54: 684–695 (in Chinese with English abstract).

[32] Wang Y, Xie J Z, Zhang H Z, et al. Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses. Theor Appl Genet, 2017, 130: 2191–2201.

[33] 杜晓宇, 邹少奎, 李楠楠, 等. 高产小麦品种周麦27号及其亲本的遗传分析. 作物杂志, 2025(6): 67–72.
Du X Y, Zou S K, Li N N, et al. Genetic analysis of high-yield wheat variety Zhoumai 27 and its parents. Crops, 2025(6): 67–72 (in Chinese with English abstract).

[1] Wang Yue-Sheng, Ge Dong-Dong, Cheng Lan-Fei, Chen Chun-Huan, Wang Chang-You, Liu Xin-Lun, Li Ting-Dong, Deng Ping-Chuan, Ji Wan-Quan, Zhao Ji-Xin. Molecular cytogenetic and disease resistance characterization of the wheat- Psathyrostachys huashanica disomic substitution line 16DH25-7 [J]. Acta Agronomica Sinica, 2026, 52(2): 433-445.
[2] JIAO Wen-Juan, BAI Bin, XIEKELAI Yilamu, ZHANG Fei-Fei, JIA Qiu-Zhen, GENG Hong-Wei, CHENG Yu-Kun. Evaluation of stripe rust resistance in 295 domestic and foreign wheat germplasm resources and molecular detection of resistance genes [J]. Acta Agronomica Sinica, 2025, 51(11): 2886-2898.
[3] QI Xue-Li, LI Ying, LI Chun-Ying, HAN Liu-Peng, ZHAO Ming-Zhong, ZHANG Jian-Zhou. Alleviative effect of salicylic acid on wheat seedlings with stripe rust based on transcriptome and differentially expressed genes [J]. Acta Agronomica Sinica, 2024, 50(4): 1080-1090.
[4] LI Yu-Jia, XU Hao, YU Shi-Nan, TANG Jian-Wei, LI Qiao-Yun, GAO Yan, ZHENG Ji-Zhou, DONG Chun-Hao, YUAN Yu-Hao, ZHENG Tian-Cun, YIN Gui-Hong. Genetic analysis of elite stripe rust resistance genes of founder parent Zhou 8425B in its derived varieties [J]. Acta Agronomica Sinica, 2024, 50(1): 16-31.
[5] LIU Dan, ZHOU Cai-E, WANG Xiao-Ting, WU Qi-Meng, ZHANG Xu, WANG Qi-Lin, ZENG Qing-Dong, KANG Zhen-Sheng, HAN De-Jun, WU Jian-Hui. Rapid identification of adult plant wheat stripe rust resistance gene YrC271 using high-throughput SNP array-based bulked segregant analysis [J]. Acta Agronomica Sinica, 2022, 48(3): 553-564.
[6] MU Wen-Yan, CHU Hong-Xin, HUANG Ning, ZHANG Lu-Lu, ZHANG Xue-Mei, GUO Zi-Kang, HUANG Cui, SUN Li-Qian, WEI Lei, LUO Yi-Nuo, WANG Zhao-Hui, LIU Jin-Shan. Relationship between grain yield and sulfur requirement characteristics of wheat cultivars (lines) in main wheat production regions of China [J]. Acta Agronomica Sinica, 2022, 48(12): 3192-3202.
[7] XI Ling, WANG Yu-Qi, ZHU Wei, WANG Yi, CHEN Guo-Yue, PU Zong-Jun, ZHOU Yong-Hong, KANG Hou-Yang. Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province [J]. Acta Agronomica Sinica, 2021, 47(7): 1309-1323.
[8] LI Ji-Fa,DENG Zhi-Ying,SUN Fu-Lai,GUAN Xi-Zhen,WANG Yan-Xun,TIAN Ji-Chun. Resistance Genes of Wheat Variety Shannong 20 Identified by Diagnostic Molecular Markers [J]. Acta Agron Sin, 2014, 40(04): 611-621.
[9] MA Dong-Fang, WANG Hai-Ge, TANG Meng-Shuang, YUAN Chi-Li, BAI Yao-Bo, ZHOU Xin-Li, SONG Jian-Rong, JING Jin-Hua. Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Zhongliang 21 [J]. Acta Agron Sin, 2011, 37(12): 2145-2151.
[10] LIANG Qiang, ZHANG Xiao-Ke, WEI Qian, WANG Xiao-Long, ZHANG Jing, SUN Dao-Jie, FU Xiao-Ji. Establishment and Application of Multiplex PCR System Based on Molecular Markers of Glutenin Subunit Genes (Loci) Related to Strong-gluten in Wheat [J]. Acta Agron Sin, 2011, 37(11): 1942-1948.
[11] CAO Shi-Qi, ZHANG Bo, LI Meng-Ju, XU Shi-Chang, JIA Hui-Sheng, JIN She-Lin, GU Qiu-Zhen, HUANG Jin, JIN Meng-An, CHANG Xun-Wu. Postulation of Stripe Rust Resistance Genes and Analysis of Adult Resistance in 50 Wheat Varieties (Lines) in Gansu Province [J]. Acta Agron Sin, 2011, 37(08): 1360-1371.
[12] LIU Xin-Ying,WANG Xiao-Jie,XUE Jie,JIA Ning,DENG Lin,CA Gao-Lei,SHANG Chun-Lei,WEI Guo-Rong,HUANG Li-Li,KANG Zhen-Sheng. Cloning and Expression Analysis of aNovel Calmodulin Isoform TaCaM5 from Wheat [J]. Acta Agron Sin, 2010, 36(06): 953-960.
[13] YANG Min-Na;XU Zhi-Bin;WANG Mei-Nan;SONG Jian-Rong;JING Jin-Xue;LI Zhen-Qi. Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Zhongliang 22 [J]. Acta Agron Sin, 2008, 34(07): 1280-1284.
[14] MA Ya-Qin;WENG Yue-Jin. Evaluation for Salt Tolerance in Spring Wheat Cultivars Introduced from Abroad [J]. Acta Agron Sin, 2005, 31(01): 58-64.
[15] WAN An-Min;NIU Yong-Chun;XU Shi-Chang;WU Li-Ren. Characteristics of Cultivars with Durable Resistance to Stripe Rust and Its Pote ntial in China [J]. Acta Agron Sin, 2000, 26(06): 751-755.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!