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Acta Agron Sin ›› 1994, Vol. 20 ›› Issue (06): 665-669.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Protoplast Culture and Plant Regeneration of Immature Cotyledons of Soybean (Glycine max L.)

Xiao Wen-yan; Wang Lian-zheng   

  1. Chinese Academy of Agricultural Sciences, Bejing, 100081
  • Received:1993-04-28 Revised:1993-12-13 Online:1994-11-12 Published:1994-11-12
  • Contact: Xiao Wen-yan

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Abstract: Thirteen soybean (Glycien max L.) genotypes were evaluated for their regenerability from protoplasts. Protoplasts were isolated from immature cotyledons, buried in beads solidified with Gellan Gum, and cultured in modified MS liquid medium containing asparagines, glutamine, allantoin, and allantoic

Key words: Soybean(Glycine max L.), Medium, Protoplast culture, Plant regeneration

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