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Acta Agron Sin ›› 2000, Vol. 26 ›› Issue (04): 406-410.

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Isolation and Sequencing Analysis of the Promoter for an Anther-specific Gene from Arabidopsis thaliana

LIU Da-Wen;XIE You-Ju;WANG Shou-Cai;DAI Jing-Rui   

  1. China Agricultural University, Beijing 100094
  • Received:1998-12-02 Revised:1999-10-08 Online:2000-07-12 Published:2000-07-12
  • Contact: LIU Da-Wen

Abstract: The upstream regulatory region of the tapetal-specific gene A9 was amplifie d fr om Arabid opsis thaliana genome by polymerase chain reaction an d cloned into HincⅡ and KpnⅠ site of pUC18. Sequence analysis showed that the cloned fragment contained 364 nucleotides, and shared a sequence homology

Key words: Arabidopsis thaliana, Promoter, Anther-s pecific expression, Genetic transformation

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