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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (06): 931-935.

• RESEARCH NOTES • Previous Articles     Next Articles

Mapping the Rice Bacterial Blight Resistance Gene Xa23 with RFLP Markers and Converting RFLP to STS Marker

FAN Ying-Lun1 2,CHEN Xue-Wei3, WANG Chun-Lian1 2, ZHU Li-Huang3, ZHANG Qi1,ZHAO Kai-Jun1 2 4   

  1. 1Key Laboratory of Crop Genetics & Breeding, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 2National Key facility for Crop Gene Resources and Genetic Improvement, Beijing 100081; 3Institute of Genetics and Developmental Biology , Chinese Academy of Sciences , Beijing,100101;4IRRI-China Office, Beijing 100081, China
  • Received:2005-05-10 Revised:1900-01-01 Online:2006-06-12 Published:2006-06-12
  • Contact: FAN Ying-Lun

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Abstract:

A F2 population derived from cross of the Xa23 near isogenic lines, JG30 and CBB23, was used for fine mapping of the rice bacterial blight resistance gene Xa23. Inoculation of the F2 plants with a Xanthomonas oryzae strain P6 showed a 3:1 ratio for the segregation of resistant and susceptible as expected. One hundred and forty-five susceptible plants were selected from the F2 population for mapping the gene Xa23 and fifteen restriction fragment length polymorphism (RFLP) markers anchored on the rice high-density map. A RFLP marker C1003A was found to be linked to the gene Xa23 by 0.4 cM. This result constitutes an important basis for map-based cloning of the gene Xa23. The RFLP marker C1003A was transformed into sequence-tagged-site (STS) marker, which can be applied for molecular marker-assisted selection (MAS) of Xa23 in rice breeding programs.

Key words: Xa23, Rice bacterial blight, RFLP markers, STS marker

CLC Number: 

  • S511
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