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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (12): 2126-2133.doi: 10.3724/SP.J.1006.2008.02126

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Expression Profiling in Roots of Wheat Cultivar “Luohan 2” under Water Stress

LI Yong-Chun1,MENG Fan-Rong2,WANG Xiao1,CHEN Lei1,REN Jiang-Ping1,NIU Hong-Bin1,LI Lei1,YIN Jun1*   

  1. 1 National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002, Henan; 2 College of Life Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2008-04-21 Revised:2008-07-15 Online:2008-12-12 Published:2008-10-10
  • Contact: YIN Jun E-mail:junyin57@yahoo.com.cn

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Abstract:

High-through microarray technology is a powerful tool for analyzing gene expression profiles of plants exposed to abiotic stresses. To investigate the molecular mechanisms of water-stress response in wheat (Triticum aestivum L.), the transcript profiles in 20% PEG 6000 treated roots and control roots of a wheat cultivar ‘Luohan 2’ were comparatively studied using the Affymetrix wheat GeneChip. Analysis of the microarray data showed that there were 3 743 genes (4 074 probe sets) induced and 4 573 genes (5 043 probe sets) repressed in roots under water-stress. Generally, the down-regulated genes were greater in quantity than up-regulated genes. Among these differentially expressed genes, 1 593 and 2 238 genes (1 716 and 2 451 probe sets) were up- and down-regulated exceeding 2 folds, respectively. Through the sourcing annotations directly from Affymetrix website (www.affymetrix.com/analysis/) in tandem with BLASTX analyzing using the Affymetrix probe set sequences, 619 candidate genes were annotated. The functional classification of annotated genes were performed based on literature searches of the functional categories described in the MIPS database and the 619 annotated genes were classified into 10 functional categories, involving in metabolism, stress response, biogenesis of cellular components, protein synthesis, cellular communication, cellular transport, transcription, cell division, protein processing, and others. Thirty-two up- or down-regulated genes with over 16-fold change ratios were annotated. A half of them belonged to the stress response category, and another five were associated with metabolism. In addition, the differential expression patterns of eight representative candidate genes were confirmed by real-time quantitative RT-PCR and the results showed that the expression changes of these candidates were generally consistent with the microarray results detected by Affymetrix GeneChip.

Key words: Water stress, Luohan 2, Gene differential expression profile, Real-time quantitative RT-PCR, Gene chip

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