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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 1087-1095.doi: 10.3724/SP.J.1006.2017.01087

• RESEARCH NOTES • Previous Articles     Next Articles

QTL Mapping for Spike Traits of Wheat Using 90k Chip Technology

WU Bing-Jin,JIAN Jun-Tao,ZHANG De-Qiang,MA Wen-Jie,FENG Jie,CUI Zi-Xia,ZHANG Chuan-Liang,SUN Dao-Jie*   

  1. College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2016-09-11 Revised:2017-03-01 Online:2017-07-12 Published:2017-04-06
  • Contact: Sun Daojie, E-mail: chinawheat@hotmail.com E-mail:wbj2010@163.com
  • Supported by:

    This study was supported by the National Key Basic Research Program of China (2014CB138100), the Natural Science Foundation of Shaanxi Province (2015JM3094), and the Key Scientific and Technological Innovation Team of Shaanxi Province (2014KCT-25).

Abstract:

Spike traits are important to grain yield in wheat. Molecular markers associated with genes/QTLs controlling spike traits are highly valuable to marker-assisted breeding. A recombinant inbred line (F8) population derived from Zhou 8425B ? Xiaoyan 81 were evaluated in three environments, and QTLs for spike length, spikelet number per spike, sterile spikelet number, grain number per spike and thousand-grain weigh were mapped into a high-density genetic map built by 90k chip. A total of 71 QTLs were located on 19 chromosomes, and the phenotype variation explained (PVE) by a single locus ranged from 2.10% to 45.25%. Thirty-seven loci were considered as main-effect QTLs owing to the PVE larger than 10%. QTLs QSl.nafu-6A.2 for spike length, QSl.nafu-7A for spike length, QSsn.nafu-2A.1 for sterile spikelet number, QSsn.nafu-2D for sterile spikelet number and QGns.nafu-2B for grain number per spike were identified repeatedly in different environments with the LOD value higher than 10 and PVE larger than 20%. QSl.nafu-6A.2 for spike length, QGns.nafu-6A for grain number per spike and QTgw.nafu-6A for thousand-grain weight were mapped in a cluster on chromosome 6A and might be applicable in marker-assisted selection because they have been detected in multiple environments and close to the loci reported.

Key words: Triticum aestivum, Spike-related traits, 90k gene chip, QTL mapping

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