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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 317-323.doi: 10.3724/SP.J.1006.2009.00317


Milling Characteristics and Distribution of Seed Storage Proteins in Rice with Various Grain shapes

ZHOU Li-Hui,LIU Qiao-Quan,GU Ming-Hong*   

  1. Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Agricultural College, Yangzhou University, Yangzhou 225009,China
  • Received:2008-08-01 Revised:2008-10-05 Online:2009-02-12 Published:2008-12-11
  • Contact: GU Ming-Hong


Rice (Oryza sativa L.), one of the most important crops, is a very good and relatively cheap source of energy and protein. The processing quality and nutrition distribution are different among rice varieties. The objective of the present study was to explore the distribution of proteins in rice kernel and to provide some references on the processing of high-quality rice using three rice cultivars with different quality and grain shapes, including two japonica cultivars Suyunuo and Yangfujing 4901, and one indica rice Qiangluzhan11. The degree of milling (DOM, 0–25%) and milling time were carefully measured, respectively. The results showed that the hardness was increased from outer to inner bran layers, and unchanged in endosperm fractions, which was higher than that of bran layers. This non-linear relationship between milling time and DOM implied variability in hardness within different rice fractions. Furthermore, the analysis of relationship between protein content and DOM indicated that the protein was not equably distributed within the brown rice kernel. The endosperm (DOM > 9%) was identified to contain most of the rice kernel proteins (about 85% of total seed proteins) in all the three cultivars. But the protein content in the brown rice kernel decreased from outer to inner fractions, and that in core endosperm fractions was only about half of that in bran layers. In addition, SDS-PAGE analysis for total proteins in the grains with various DOM showed that the distribution of seed protein components was relatively uniform, suggesting the synchro genetic expression of seed protein components in rice grain.

Key words: Rice(Oryza sativa L.), Milling characteristics, Seed storage proteins, Grain quality

[1] Juliano B O. Rice: Chemistry and Technology. St. Paul, Minn (USA): American Association of Cereal Chemists Inc., 1985. pp 1–174
[2] Liu Q-Q(刘巧泉), Zhou L-H(周丽慧), Wang H-M(王红梅), Gu M-H(顾铭洪). Advances on biosynthesis of rice seed storage proteins in molecular biology. Mol Plant Breed (分子植物育种), 2008, 6(1): 1–15 (in Chinese with English abstract)
[3] Wang H-M(王红梅), Liu Q-Q(刘巧泉), Gu M-H(顾铭洪). The nutritional quality of rice proteins and its genetic improvement. Plant Physiol Commun (植物生理学通讯), 2007, 43(2): 391–396 (in Chinese)
[4] Itani T, Tamaki M, Arai E, Horino T. Distribution of amylase, ni-trogen, and minerals in rice kernels with various characters. J Agric Food Chem, 2002, 50: 5326–5332
[5] Kennedy B M, Schelstrate M, Del Rosario A R. Chemical, physical, and nutritional properties of high-protein flours and re-sidual kernel from the overmilling of uncoated milled rice: 1. Milling procedure and protein, fat, ash, amylase, and starch con-tent. Cereal Chem, 1974, 51: 435–448
[6] Resurreccion A P, Juliano B O, Tanaka Y. Nutrient content and distribution in milling fractions of rice grain. J Sci Food Agric, 1979, 30: 475–481
[7] Bradbury J H, Collins J G, Pyliotis N A. Methods of separation of the major histological components of rice and characterization of their proteins by amino acid analysis. Cereal Chem, 1980, 57: 133–137
[8] Cagampang G B, Crruz L J. Studies on the extraction and com-position of rice proteins. Cereal Chem, 1966, 43: 145–155
[9] Furukawa S, Mizuma T, Kiyokawa Y, Masumura T, Tanaka K, Wakai Y. Distribution of storage proteins in low-glutelin rice seed determined using a fluorescent Antibody. J Biosci Bioeng, 2003, 96: 467–473
[10] Wang Y, Wang L, Shepard D, Wang F, Patindol J. Properties and structures of flours and starches from whole, broken and yel-lowed rice kernels in a model study. Cereal Chem, 2002, 79: 383–386
[11] Chen H, Siebenmorgen T J. Effect of rice kernels thickness on degree of milling and associated optical measurements. Cereal Chem, 1997, 74: 821–825
[12] Brenda G L, Elaine T, Champagne C, Bryan T V, William R W, Franklin E B II, Bill D W, Anna M M, Karen A M, Steve L, Kent S M, David E K. Effect of degree of milling, drying condition and final moisture content on sensory texture of cooked rice. Cereal Chem, 1999, 76: 56–62
[13] Stermer R A. An instrument for objective measurements of de-gree of milling and color of milled rice. Cereal Chem, 1968, 45: 358–364
[14] Wadsworth J I. Rice: Science and Technology. New York: Marcel Dekker, Inc. 1994. pp 139–176
[15] Association of Official Analytical Chemists (AOAC). Association of Official Analytical Chemists Official Methods of Analysis, 16th edn. Method 990.03, Washington, DC: The Association, 1995. pp 35–43
[16] Yamagata H, Sugimoto T, Tanaka K, Kasai Z. Biosynthesis of storage proteins in developing rice seeds. Plant Physiol, 1982, 70: 1094–1100
[17] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Labo-ratory Manual. Cold Spring Harbor, US, NY: Cold Spring Harbor Laboratory Press, 1989
[18] Indudhara S Y M, Sowbhagya C M, Bhattacharya K R. Changes in the physicochemical properties of rice with aging. J Sci Food Agric, 1978, 29: 627–631
[19] Villareal R M, Resurreccion A P, Suzuki L B, Juliano B O. Changes in the physicochemical properties of rice during storage. Starke, 1976, 28: 88–92
[20] Nagato K. On the hardness of rice endosperm. Proc Crop Sci Jpn, 1962, 31: 102–105 (in Japanese with English abstract)
[21] Rout G, Senapati B, Ahmed T. Studies in relative susceptibility of some high-yield varieties of rice to the weevil, Sitophilus orazae L. Bull Grain Technol, 1976, 14: 34
[22] Goodman D E, Rao R M. Experimentally validated predictive models for puffability of gelatinized rice. La. Agric. Exp. Stn. Bull. 753. La. State Univ. Agric. Ctr. Baton Rouge, LA. 1983. pp 58–79
[23] Webb B D, Pomeranz Y, Afework S, Lai F S, Bollich C N. Rice grain hardness and its relationship to some milling, cooking, and processing characteristics. Cereal Chem, 1986, 63: 27–30
[24] Lieve L, Els D B, Greet E V, Vandeputte W S, Veraverbeke V D, Walter De M, Delcour J A. Effect of milling on colour and nutri-tional properties of rice. Food Chem, 2007, 100: 1496–1503
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