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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1336-1341.doi: 10.3724/SP.J.1006.2010.01336


Tissue Speciality and Stress Responses in Expression of Three WAX2 Homologous Genes in Rice

GAO Guo-Fu1,2,ZOU Jie1,ZHOU Xiao-Yun1,LIU Ai-Ling1,3,WEI Bao-Yang3,CHEN Xin-Bo1,3,*   

  1. 1 Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China; 2 Research Institute of Science and Technology Information, Hunan Academy of Agricultural Sciences, Changsha 410125,China; 3 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-02-09 Revised:2010-04-21 Online:2010-08-12 Published:2010-05-20
  • Contact: CHEN Xin-Bo。E-mail: xinbochen@live.cn

Abstract: Plant surface is covered by epidermis with a layer of wax. It can regulate non-stomatal water loss, protect against ultraviolet radiation damage and germ invasion. WAX2 gene in Arabidopsis is involved in cuticular wax production and significantly affects leaf water evaporation. Three WAX2 homologous genes in rice, named OsWAX2-1, OsWAX2-2, OsWAX2-3 were found by BLAST search in the NCBI database, with the homology of 56.0%, 55.2%, and 52.0% to Arabidopsis WAX2 gene respectively. Homology of their predicted proteins to Arabidopsis WAX2 protein was 61.5%, 60.5%, and 64.7%, respectively. All of the three proteins had four transmembrane domains and a conserved sterol desaturase domain, suggesting that OsWAX2 proteins are transmembrane proteins and may be related to plant cuticular wax biosynthesis. Expression patterns of the three rice WAX2 homologs were analyzed using semi-quantitative RT-PCR. The results showed that the three OsWAX2 genes had tissue speciality in expression. They also exhibited different expression patterns under high-temperature, low temperature, NaCl, PEG and ABA treatments. These results could be useful for further functional characterization of the three OsWAX2 genes.

Key words: Rice, WAX2 homologous genes, Semi-quantitative RT-PCR, Expression, Stress response

   [1]    Richards R A, Rawson H M, Johnson D A. Glaucousness in wheat: its development and effect on water-use efficiency, gas exchange and photosynthetic tissue temperatures. Aust J Plant Physiol, 1986, 13: 465–473   
[2]    Johnson D A, Richard R A, Turner N C. Yield, water relations, gas exchange and surface reflectance of near isogenic wheat lines differing in glaucousness. Crop Sci, 1983, 23: 318–323   
[3]    Rawson H M, Clarke J M. Nocturnal transpiration in wheat. Aust J Plant Physiol, 1988, 15: 387–406   
[4]    Chen X B, Goodwin S M, Boroff V L, Liu X L, Jenks M A. Cloning and characterization of the WAX2 gene of Arabidopsis involved in cuticle membrane and wax production. Plant Cell, 2003, 15: 1170–1185   
[5]    Kurata J, Kawabata-Awai C, Sakuradani E, Shimizu S, Okada K, Wada T. The YORE-YORE gene regulates multiple aspects of epi dermical cell differentiation in Arabidopsis. Plant J, 2003, 36: 55–66   
[6]    Sanchez F J, Manzanares M, Andres E F, Tenorio J L, Ayerbe L. Residual transpiration rate, epicuticular wax loadand leaf colour of pea plants in drought conditions: influence on harvest index and canopy temperature. Eur J Agron, 2001, 15: 57–70   
[7]    Xiong L, Schumaker K S, Zhu J K. Cell signaling during cold, drought, and salt stress. Plant Cell, 2002, 14(suppl): 165–183   
[8]    Sanchez F J, Manzanares M, Andres E F, Tenorio J L, Ayerbe L. Residual transpiration rate, epicuticular wax loadand leaf colour of pea plants in drought conditions. Influence on harvest index and canopy temperature. Eur J Agron,2001, 15: 57–70   
[9]    Samdur M Y, Manivel P, Jain V K, Chikani B M, Gor H K, Desai S, Misra J B. Genotypic differences and water-deficit induced enhancement in epicuticular wax load in peanut. Crop Sci, 2003, 43: 1294–1299
[10]    Zhang J Y, Broeckling C D, Blancaflor E B, Sledge M K, Sumner L W, Wang Z Y. Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). Plant J, 2005, 42: 689–707
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