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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1336-1341.doi: 10.3724/SP.J.1006.2010.01336

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Tissue Speciality and Stress Responses in Expression of Three WAX2 Homologous Genes in Rice

GAO Guo-Fu1,2,ZOU Jie1,ZHOU Xiao-Yun1,LIU Ai-Ling1,3,WEI Bao-Yang3,CHEN Xin-Bo1,3,*   

  1. 1 Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China; 2 Research Institute of Science and Technology Information, Hunan Academy of Agricultural Sciences, Changsha 410125,China; 3 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-02-09 Revised:2010-04-21 Online:2010-08-12 Published:2010-05-20
  • Contact: CHEN Xin-Bo。E-mail: xinbochen@live.cn

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Abstract: Plant surface is covered by epidermis with a layer of wax. It can regulate non-stomatal water loss, protect against ultraviolet radiation damage and germ invasion. WAX2 gene in Arabidopsis is involved in cuticular wax production and significantly affects leaf water evaporation. Three WAX2 homologous genes in rice, named OsWAX2-1, OsWAX2-2, OsWAX2-3 were found by BLAST search in the NCBI database, with the homology of 56.0%, 55.2%, and 52.0% to Arabidopsis WAX2 gene respectively. Homology of their predicted proteins to Arabidopsis WAX2 protein was 61.5%, 60.5%, and 64.7%, respectively. All of the three proteins had four transmembrane domains and a conserved sterol desaturase domain, suggesting that OsWAX2 proteins are transmembrane proteins and may be related to plant cuticular wax biosynthesis. Expression patterns of the three rice WAX2 homologs were analyzed using semi-quantitative RT-PCR. The results showed that the three OsWAX2 genes had tissue speciality in expression. They also exhibited different expression patterns under high-temperature, low temperature, NaCl, PEG and ABA treatments. These results could be useful for further functional characterization of the three OsWAX2 genes.

Key words: Rice, WAX2 homologous genes, Semi-quantitative RT-PCR, Expression, Stress response

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