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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (09): 1490-1497.doi: 10.3724/SP.J.1006.2010.01490

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Screening Wheat cultivars with Genetic Similarity Using Molecular Markers

WANG Li-Xin1,LI Hong-Bo1,LIAO Qin2,QIU Jun2,CHANG Li-Fang1,LIU Li-Hua1,REN Li-Ping1,GAO Xin-Huan1,ZHAO Chang-Ping1*   

  1. 1Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing 100097, China；National Agriculture Technology Spreading Service, Beijing 100026, China
  • Received:2010-01-08 Revised:2010-04-17 Online:2010-09-12 Published:2010-06-11
  • Contact: ZHAO Chang-Ping, E-mail: bjhwc2003@yahoo.com.cn, Tel: 010-51503968

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Abstract: Cultivars with similar traits are often found in the national winter wheat (Triticum aestivum L.) regional trials. Molecular markers have been introduced to identify such cultivars efficiently and accurately in screening wheat cultivars with genetic similarity. In this study, 681 wheat cultivars and lines were assessed using 105 molecular markers, including 63 genome-SSRs, 21 EST-SSRs, and 21 AFLP-SCARs. These markers were classified into core primer (21 pairs), first-grade standby primer (29 pairs), and second-grade standby primer (55 pairs). The 681 cultivars and lines consisted of 134 released cultivars and 547 lines evaluated in the national winter wheat regional trials from 2005 to 2009. Cultivars were primarily screened with the core primers, and those with similar fingerprint in 19-21 loci were further tested with the first-grade standby primers, and only the confirmed similar cultivars (GS > 0.90) were conduced the final detection by the second-grade standby primers. Finally, the cultivars with total GS value (at least 100 loci) higher than 0.95 were considered as similar ones. These similar cultivars were continuously identified on the basis of main agronomic traits. The results showed that 95% of the similar cultivars detected by molecular markers had identical or highly similar agronomic traits, and only two pairs of cultivars with GS > 0.95 had distinct performances in agronomic traits. Thus, the laboratory identification had the accordance with the field test by 95%, suggesting that these molecular markers are effective in bulk screening similar cultivars in wheat regional trials.

Key words: Winter wheat, molecular marker, genomic similarity coefficient

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