Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 1012-1019.doi: 10.3724/SP.J.1006.2011.01012

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression of GhSAMS Gene Related to Salt-tolerance in Gossypium hirsutum L.

ZHOU Kai,SONG Li-Yan,YE Wu-Wei*,WANG Jun-Juan,WANG De-Long,FAN Bao-Xiang   

  1. Cotton Research Institute, Chinese Academy of Agricultural Science, Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry, Anyang 455000, China
  • Received:2010-11-01 Revised:2011-03-28 Online:2011-06-12 Published:2011-04-12

PDF

1765

Cited

2

Abstract: As one of main abiotic stresses in nature, salt stress does great harm to plants, and seriously affect plant growth and development. Simultaneously, the crops cultivated in the saline land undergo a wide range of yield decline. To excavate salt-tolerance gene, we cloned the cDNA of S-adenosyl-L-methionine synthetase gene from Gossypium hirsutum by RACE and RT-PCR, which was named GhSAMS, with the cDNA full length of 1 576 bp, ORF of 1 182 bp, and coding 393 amino acid residues. Bioinformatics analysis showed GhSAMS has the similarity of 91%, 93%, and 93% with Arabidopsis thaliana, Suaeda salsa, and Oryza sativa, respectively. Phylogenetic analysis showed GhSAMS was the closest to Suaeda salsa,and Real-time PCR suggested that GhSAMS was induced by salt stress, while the induction was postponed in salt sensitivity material. It showed lower gene expression level on salt sensitive material Zhong S9612 relative to salt resistance material Zhong 9835. At the same time, we established protokaryotic expression vector pET28-GhSAMS and transformed GhSAMS into E. coli after IPTG induction, showing a successful gene expression.

Key words: Gossypium hirsutum, Salt stress, S-adenosyl-L-methionine synthetase, Prokaryotic expression

[1]Guo Y H, Yu Y P, Wang D, Wu C A, Yang D G, Huang J G, Zheng C C. GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5. New Phytologist, 2009, 183: 62–75
[2]Huang B, Jin L, Liu J Y. Identification and characterization of the novel gene GhDBP2 encoding a DRE-binding protein from cotton (Gossypium hirsutum). J Plant Physiol, 2008, 165: 214–223
[3]Yang Y-W(杨郁文), Ni W-C(倪万潮), Zhang B-L(张保龙), Shen X-L(沈新莲), Zhang X-G(张香桂), Xu Y-J(徐英俊), Yao S(姚姝). Molecular cloning and expression analysis of a serine/threonine protein kinase gene in upland cotton. Cotton Sci (棉花学报), 2006, 18(3): 140–144 (in Chinese with English abstract)
[4]Wu C A, Yang G D, Meng Q W, Zheng C C. The cotton GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays an important role in salt stress. Plant Cell Physiol, 2004, 45: 600–607
[5]Chen Y-J(陈亚娟). Isolation and Characterization of GaP5CS and GaTPS in Gossypium arboretum L. MS Dissertation of Chinese Academy of Agricultural Sciences, 2009 (in Chinese with English abstract)
[6]Baker J, Steele C, Dure L III. Sequence and characterization of 6 lea proteins and their genes from cotton. Plant Mol Biol, 1988, 11: 277–291
[7]Yang S F, Hoffman N E. Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol, 1984, 35: 155–189
[8]Tabor C W, Tabor H. Methionine adenosyltransferase (S-adenosylmethionine synthetase) and S-adenosylmethionine decarboxylase. Adv Enzymol Related Areas Mol Biol, 1984, 56: 251–282
[9]Sánchez-Aguayo I, Rodriguez-Galan J M, García R, Torreblanca J, Pardo J M. Salt stress enhances xylem development and expression of S-adenosyl-L-methionine synthase in lignifying tissues of tomato plants. Planta, 2004, 220: 278–285
[10]Markham G D, Hafner E W, Tabor C W, Tabor H. S-adenosylmethionine synthetase from Escherichia coli. J Biol Chem, 1980, 255: 9082
[11]Peleman J, Boerjan W, Engler G, Seurinck J, Botterman J, Alliotte T, Montagu M V, Inzé D. Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetasee. Plant Cell, 1989, 1: 81–93
[12]Thomas D, Surdin-Kerjan Y. SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. J Biol Chem, 1987, 362: 16704–16709
[13]Schröder G, Eichel J, Breinig S, Schröder J. Three defferentially expressed S-adenosylmethionine synthetases from Catharanthus roseus: molecular and functional characterization. Plant Mol Biol, 1997, 33: 211–222
[14]Breusegem F V, Dekeyser R, Gielen J, Montagu M V, Caplan A. Characterization of a S-adenosylmethionine synthetase gene in rice. Plant Physiol, 1994, 105: 1463–1464
[15]Zhaki A, shoseyov O, Weiss D. A petunia cDNA encoding S-adenosylmethionine synthetase. Plant Physiol, 1995, 108: 841–842
[16]Doorsselaere J V, Gielen J, Montagu M V, Inzé D. A cDNA encoding S-adenosyl-L-methionine synthetase from poplar. Plant Physiol, 1993, 102: 1365–1366
[17]Larsen P B, Woodson W R. Cloning and nucleotide sequence of an S-adenosylmethionine synthetase cDNA from Carnation. Plant Physiol, 1991, 96: 997–999
[18]Wen C M, Wu M, Goh C J, Pua E C. Cloning and nucleotide sequence of a cDNA encoding S-adenosyl-L-methionine synthetase from mustard (Brassica juncea). Plant Physiol, 1995, 107: 1021–1022
[19]Espartero J, Pintor-Toro J A, Pardo J M. Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress. Plant Mol Biol, 1994, 25: 217–227
[20]Ma X L, Wang Z L, Qi Y C, Zhao Y X, Zhang H. Isolation of S-adenosylmethionine synthetase gene from Suaeda salsa and its differential expression under NaCl stress. J Integ Plant Biol, 2003, 45: 1359–1365
[21]Qi Y C, Wang F F, Zhang H, Liu W Q. Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco. Acta Physiol Plant, 2009, 32: 263–269
[22]Ye W-W(叶武威), Liu J-D(刘金定). Technique and application on salt-tolerance appraisal of cotton germplasm resources. China Cotton (中国棉花), 1998, 25(9): 34–38 (in Chinese)
[23]Salzman R A, Fujita T, Salzman K Z, Hasegawa P M, Bressan R A. An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol Biol Rep, 1999, 17: 11–17
[24]Jiang J-X(蒋建雄), Zhang T-Z(张天真). Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method. Cotton Sci (棉花学报), 2003, 15(3): 166–167 (in Chinese with English abstract)
[25]Ye W-W(叶武威), Zhao Y-L(赵云雷), Wang J-J(王俊娟), Fan B-X(樊保相). Construction of SSH library on root system of salinity-tolerance variety (G. hirsutum L.) under the stress of salinity. Cotton Sci (棉花学报), 2009, 21(5): 339–345 (in Chinese with English abstract)
[26]Levitt J. Responses of Plants to Environmental Stresses: Chilling, Freezing, and High Temperature Stresses. New York: Academic Press, 1980
[27]Shen F-F(沈法富), Yu Y-J(于元杰), Bi J-J(毕建杰), Liu F-Z(刘凤珍), Yin C-Y(尹承佾). A diallel analysis of salt tolerance in upland cotton. Acta Agron Sin (作物学报), 2001, 27(1): 50–54 (in Chinese with English abstract)
[28]Hua Y(化烨). GsSAMS Gene Transformation into Alfalfa and Cultivation of Transformation New Lines. MS Dissertation of Northeast Agricultural University, 2009 (in Chinese with English abstract)
[29]Yang J-L(杨金丽), Zhao X-M(赵小明), Yin H(尹恒), Zhang H-Y(张洪艳), Du Y-G(杜昱光). Analysis of proteins interacted with OIPK by yeast two-hybrid method. Chin J Appl Environ Biol (应用与环境生物学报), 2010, 16(4): 474–477 (in Chinese with English abstract)
[30]Qi Y-C(戚元成), Ma L(马雷), Wang F-F(王菲菲), Liu W-Q(刘卫群). Overexpression of S-adenosylmethionine synthetase promote polyamine biosynthesis in transgenic tobacco. Plant Physiol Commun (植物生理学通讯), 2009, 45(8): 791–793 (in Chinese with English abstract)
[31]Fan J-P(樊金萍), Bai Xi(柏锡), Li Yong(李勇), Ji W(纪巍), Wang X(王希), Cai H(才华), Zhu Y-M(朱延明). Cloning and function analysis of gene SAMS from Glycine soja. Acta Agron Sin (作物学报), 2008, 34(9):1581–1587 (in Chinese with English abstract)
[1] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[2] Song Yu-Zhen, Bheel Chander Kumar, Wang Yue, Zhang Ying-Xing, Guo Juan, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Genome-wide identification of the AP2 subfamily in broomcorn millet and functional characterization of PmAP2-1 and PmAP2-9 in salt tolerance [J]. Acta Agronomica Sinica, 2026, 52(4): 1127-1139.
[3] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[4] Zhang Qing, Yang Yu, Guo Qian, Yue Pei-Yao, Yin Cong-Cong, Niu Jing-Ping, Zhao Jin-Zhong, Du Wei-Jun, Yue Ai-Qin. Cloning and functional analysis of the soybean GmARA6a gene in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 480-493.
[5] Liu Ji-Chang, Li Si-Ye, Li Xue-Ting, Wang Hong-Zhang, Liu Peng, Zhang Ji-Wang, Zhao Bin, Ren Bai-Zhao, Ren Hao. Effects of salt stress on root growth and nutrient absorption efficiency of different salt-tolerant summer maize varieties [J]. Acta Agronomica Sinica, 2026, 52(2): 565-577.
[6] LI Xue-Ting, REN Hao, WANG Hong-Zhang, ZHANG Ji-Wang, ZHAO Bin, REN Bai-Zhao, LIU Ying, YAO Hai-Yan, LIU Peng. Effects of salt stress on photosynthetic performance and dry matter accumulation and distribution in leaves of different salt-tolerant maize varieties [J]. Acta Agronomica Sinica, 2025, 51(4): 1091-1101.
[7] PAN Ju-Zhong, WEI Ping, ZHU De-Ping, SHAO Sheng-Xue, CHEN Shan-Shan, WEI Ya-Qian, GAO Wei-Wei. Cloning and functional analysis of OsERF104 transcription factor in rice [J]. Acta Agronomica Sinica, 2025, 51(4): 900-913.
[8] HUO Ru-Xue, GE Xiang-Han, SHI Jia, LI Xue-Rui, DAI Sheng-Jie, LIU Zhen-Ning, LI Zong-Yun. Functional analysis of the sweetpotato histidine kinase protein IbHK5 in response to drought and salt stresses [J]. Acta Agronomica Sinica, 2025, 51(3): 650-666.
[9] MENG Fan-Hua, LIU Min, SHEN Ao, LIU Wei. Preliminary investigation of the SiLTP1: a lipid transfer protein gene involved in the salt tolerance of foxtail millet [J]. Acta Agronomica Sinica, 2025, 51(1): 58-67.
[10] LI Wen-Juan, WANG Li-Min, QI Yan-Ni, ZHAO Wei, XIE Ya-Ping, DANG Zhao, ZHAO Li-Rong, LI Wen, XU Chen-Meng, WANG Yan, ZHANG Jian-Ping. Functional analysis of flax LuWRI1a in response to drought and salt stresses [J]. Acta Agronomica Sinica, 2024, 50(7): 1750-1761.
[11] SHE Meng, ZHENG Deng-Yu, KE Zhao, WU Zhong-Yi, ZOU Hua-Wen, ZHANG Zhong-Bao. Cloning and functional analysis of ZmGRAS13 gene in maize [J]. Acta Agronomica Sinica, 2024, 50(6): 1420-1434.
[12] WANG Long, LI Jing, QIAN Chen, LIN Guo-Bing, LI Yi-Yang, YANG Guang, ZUO Qing-Song. Effects of salt stress on yield, quality, and physiology in rapeseed [J]. Acta Agronomica Sinica, 2024, 50(6): 1597-1607.
[13] ZHANG Jin-Hui, XIAO Zi-Yi, LI Xu-Hua, ZHANG Ming, JIA Chun-Lan, PAN Zhen-Yuan, QIU Fa-Zhan. Salt tolerance evaluation and transcriptome analysis of maize mutant caspl2b2 [J]. Acta Agronomica Sinica, 2024, 50(12): 3144-3154.
[14] GUO Jia-Xin, YE Yang, GUO Hui-Juan, MIN Wei. Effects and variability analysis of different salt and alkali stresses on the proteome of cotton leaves [J]. Acta Agronomica Sinica, 2024, 50(1): 219-236.
[15] YANG Chuang, WANG Ling, QUAN Cheng-Tao, YU Liang-Qian, DAI Cheng, GUO Liang, FU Ting-Dong, MA Chao-Zhi. Relative expression profiles of genes response to salt stress and constructions of gene co-expression networks in Brassica napus L. [J]. Acta Agronomica Sinica, 2024, 50(1): 237-250.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd