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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 1012-1019.doi: 10.3724/SP.J.1006.2011.01012

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression of GhSAMS Gene Related to Salt-tolerance in Gossypium hirsutum L.

ZHOU Kai,SONG Li-Yan,YE Wu-Wei*,WANG Jun-Juan,WANG De-Long,FAN Bao-Xiang   

  1. Cotton Research Institute, Chinese Academy of Agricultural Science, Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry, Anyang 455000, China
  • Received:2010-11-01 Revised:2011-03-28 Online:2011-06-12 Published:2011-04-12

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Abstract: As one of main abiotic stresses in nature, salt stress does great harm to plants, and seriously affect plant growth and development. Simultaneously, the crops cultivated in the saline land undergo a wide range of yield decline. To excavate salt-tolerance gene, we cloned the cDNA of S-adenosyl-L-methionine synthetase gene from Gossypium hirsutum by RACE and RT-PCR, which was named GhSAMS, with the cDNA full length of 1 576 bp, ORF of 1 182 bp, and coding 393 amino acid residues. Bioinformatics analysis showed GhSAMS has the similarity of 91%, 93%, and 93% with Arabidopsis thaliana, Suaeda salsa, and Oryza sativa, respectively. Phylogenetic analysis showed GhSAMS was the closest to Suaeda salsa,and Real-time PCR suggested that GhSAMS was induced by salt stress, while the induction was postponed in salt sensitivity material. It showed lower gene expression level on salt sensitive material Zhong S9612 relative to salt resistance material Zhong 9835. At the same time, we established protokaryotic expression vector pET28-GhSAMS and transformed GhSAMS into E. coli after IPTG induction, showing a successful gene expression.

Key words: Gossypium hirsutum, Salt stress, S-adenosyl-L-methionine synthetase, Prokaryotic expression

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