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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 589-595.doi: 10.3724/SP.J.1006.2012.00589

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Chromosome Location and Molecular Mapping of Powdery Mildew Resistance Gene PmAS846 Originated from Wild Emmer (Triticum turgidum var. dicoccoides)

XUE Fei1,WANG Chang-You1,ZHANG Li-Hua2,ZHANG Hong1,LI Hao1,WANG Ya-Juan1,LIU Xin-Lun1,JI Wan-Quan1,*   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China; 2 Seed Technology Extension Station of Neixiang Country in Henan Province, Neixiang 474350, China
  • Received:2011-08-08 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-14
  • Contact: 王长有, E-mail: chywang2004@126.com; 吉万全, E-mail: jiwanquan2003@126.com

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Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most important diseases of wheat (Triticum aestivum L.) worldwide. Wheat relativesare important donors of resistance genes against this disease in wheat breeding program. The common wheat line N9738 is highly resistant to Bgt isolate E09 and Shaanxi prevailing races at both seedling and adult plant stages. The PmAS846 gene in line N9738 was derived from wild emmer (Triticum turgidum var. dicoccoides) accession As846. Genetic analysis of an F2 population and their F3 families, developed from the cross between N9738 and a susceptible common wheat cultivar Huixianhong, indicated that N9738 carries one dominant resistance gene. A set of common wheat nullisomic (monosomic) lines were used to analyze the chromosomal location of PmAS846. The results revealed that PmAS846 was located on wheat chromosome 5B. Microsatellite markers on wheat chromosome 5B were used to map the gene using bulked segregant analysis. Eleven microsatellite markers were used to construct a linkage map for the gene, and two markers, Xgpw3191 and Xfcp1, ?anking PmAS846 location at 7.3 and 1.8 cM, respectively. Amplification of 5B chromosome deletion lines of Chinese Spring with the flanking markers mapped PmAS846 on chromosome 5BL bin 0.75–0.76. Based on expressed sequence tags (ESTs) information mapped on chromosome 5BL bin 0.75–0.76, we identified EST-derived sequence tagged site (STS) markers BF202652 and BF482522 to be closely linked to PmAS846 with genetic distance of 5.1 cM both. These markers can be used in fine mapping of PmAS846 and marker-assisted selection.

Key words: Powdery mildew, Wild emmer, Resistance gene, Chromosome location, Molecular markers

[1]Huang X, Röder M S. Molecular mapping of powdery mildew resistance genes in wheat: A review. Euphytica, 2004, 137: 203-223

[2]Lillemo M, Asalf B, Singh R P, Huerta-Espino J, Chen X M, He Z H, Bjørnstad Å. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet, 2008, 116: 1155-1166

[3]Perugini L D, Murphy J P, Marshall D, Brown-Guedira G. Pm37, a new broadly effective powdery mildew resistance gene from Triticum timopheevii. Theor Appl Genet, 2008, 116: 417-425

[4]He R, Chang Z, Yang Z, Yuan Z, Zhan H, Zhang X, Liu J. Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet, 2009, 118: 1173-1180

[5]Ma H, Kong Z, Fu B, Li N, Zhang L, Jia H, Ma Z. Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat. Theor Appl Genet, 2011, 123: 1099-1106

[6]Hao Y, Liu A, Wang Y, Feng D, Gao J, Li X, Liu S, Wang H. Pm23: A new allele of Pm4 located on chromosome 2AL in wheat. Theor Appl Genet, 2008, 117: 1205-1212

[7]Xie W, Nevo E. Wild emmer. genetic resources, gene mapping and potential for wheat improvement. Euphytica, 2008, 164: 603-614

[8]Özkan H, Willcox G, Graner A, Salamini F, Kilian B. Geographic distribution and domestication of wild emmer wheat (Triticum dicoccoides). Genet Resour Crop Evol, 2010, 58: 11-53

[9]Reader S M, Miller T E. The introduction into bread wheat of a major gene for resistance to powdery mildew from wild emmer wheat. Euphytica, 1991, 53: 57-60

[10]Rong J K, Millet E, Manisterski J, Feldman M. A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP based mapping. Euphytica, 2000, 115: 121-126

[11]Liu Z, Sun Q, Ni Z, Nevo E, Yang T. Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica, 2002, 123: 21-29

[12]Blanco A, Gadaleta A, Cenci A, Carluccio A V, Abdelbacki A M M, Simeone R. Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theor Appl Genet, 2008, 117: 135-142

[13]Li G, Fang T, Xie C, Yang T, Nevo E. Molecular characterization of a new powdery mildew resistance gene Pm41 on chromosome 3BL derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet, 2009, 119: 531-539

[14]Hua W, Liu Z, Zhu J, Xie C, Yang T, Zhou Y, Duan X, Sun Q. 2009. Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet, 119: 223-230

[15]Qi L L, Echalier B, Chao S, Lazo G R, Butler G E, Anderson O D, Akhunov E D, Dvorak J, Linkiewicz A M, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis C E, Greene R A, Kantety R, La Rota C M, Munkvold J D, Sorrells S F, Sorrells M E, Dilbirligi M, Sidhu D, Erayman M, Randhawa H S, Sandhu D, Bondareva S N, Gill K S, Mahmoud A A, Ma X F, Miftahudin, Gustafson J P, Conley E J, Nduati V, Gonzalez-Hernandez J L, Anderson J A, Peng J H, Lapitan N L V, Hossain K G, Kalavacharla V, Kianian S F, Pathan M S, Zhang D S, Nguyen H T, Choi D W, Fenton R D, Close T J, McGuire P E, Qualset C O, Gill B S. A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics, 2004, 168: 701-712

[16]Qin B, Cao A, Wang H, Chen T, You F M, Liu Y, Ji J, Liu D, Chen P, Wang X E. Collinearity-based marker mining for the fine mapping of Pm6, a powdery mildew resistance gene in wheat. Theor Appl Genet, 2011, 123: 207-218

[17]Wang C-Y(王长有) Ji W-Q(吉万全), Zhang G-S(张改生), Wang Q-Y(王秋英), Cai D-M(蔡东明), Xue X-Z(薛秀庄). SSR markers and preliminary chromosomal location of a powdery mildew resistance gene in common wheat germplasm N9134. Acta Agron Sin (作物学报), 2007, 33(1): 163-166 (in English with Chinese abstract)

[18]Sheng B-Q(盛宝钦). Scoring powdery mildew with infection type at wheat seedling stage. Plant Prot (植物保护), 1988, (1): 49 (in Chinese)

[19]Saari E E, Prescott J M. A scale for appraising the foliar intensity of wheat diseases. Plant Dis Rep, 1975, 59: 377-380

[20]Saghai-Maroof M A, Soliman K M, Jorgensen R A, Allard R W. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA, 1984, 81: 8014-8018

[21]Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109:1105-1114

[22]Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M-Hln, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007-2023

[23]Liu R-H(刘仁虎), Meng J-L(孟金陵). MapDraw: a microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas (遗传), 2003, 25(3): 317-321 (in Chinese with English abstract)

[24]Sears E R. The aneuploids of common wheat. Mol Agric Exp Stn Res Bull, 1954, 572: 1-58

[25]Hsam S L K, Lapochkina I F, Zeller F J. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.): 8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica, 2003, 133: 367-370

[26]Zeller F J, Kong L, Hartl L, Mohler V, Hsam S L K. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.): 7. Gene Pm29 in line Pova. Euphytica, 2002, 123: 187-194

[27]Peusha H, Enno T, Priilinn O. Chromosomal location of powdery mildew resistance genes and cytogenetic analysis of meiosis in common wheat cultivar Meri. Hereditas, 2000, 132: 29-34

[28]Huang X Q, Hsam S L K, Zeller F J. Chromosomal location of powdery mildew resistance genes in Chinese wheat (Triticum aestivum L. em. Thell.) landraces Xiaobaidong and Fuzhuang 30. Genet Breed, 2000, 54: 311-317

[29]Faris J, Anderson J, Francl L, Jordahl J. Chromosomal location of a gene conditioning insensitivity in wheat to a necrosis-inducing culture filtrate from Pyrenophora tritici-repentis. Phytopathology, 1996, 86: 459-463

[30]Faris J D, Haen K M, Gill B S. Saturation mapping of a gene-rich recombination hot spot region in wheat. Genetics, 2000, 154:823-835

[31]The International Brachypodium Initiative. Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature, 2010, 463: 763-768

[32]Linkiewicz A M, Qi L L, Gill B S, Ratnasiri A, Echalier B, Chao S, Lazo G R, Hummel D D, Anderson O D, Akhunov E D, Dvorak J, Pathan M S, Nguyen H T, Peng J H, Lapitan N L V, Miftahudin, Gustafson J P, La Rota C M, Sorrells M E, Hossain K G, Kalavacharla V, Kianian S F, Sandhu D, Bondareva S N, Gill K S, Conley E J, Anderson J A, Fenton R D, Close T J, McGuire P E, Qualset C O, Dubcovsky J. A 2500-locus bin map of wheat homoeologous group 5 provides insights on genedistribution and colinearity with rice. Genetics, 2004, 168: 665-676

[33]Zhang H, Guan H, Li J, Zhu J, Xie C, Zhou Y, Duan X, Yang T, Sun Q, Liu Z. Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.). Theor Appl Genet, 2010, 121: 1613-1621
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