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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1471-1482.doi: 10.3724/SP.J.1006.2012.01471

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Differential Expressions of the Proteins Related to Grain Filling between Superior and Inferior Spikelets of Super Rice after Anthesis

CHEN Ting-Ting,TAN Gui-Lu,CHU Guang,LIU Li-Jun,YANG Jian-Chang*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China
  • Received:2012-01-13 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-04
  • Contact: 杨建昌, E-mail: jcyang@yzu.edu.cn

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Abstract: Poor grain filling of the later-flowered inferior spikelets (in contrast to the earlier-flowered superior spikelets) is a serious problem in rice production. This problem is more aggravated in new bred super rice cultivars. To better understand the mechanism underlying the poor grain filling of inferior spikelets, we investigated the difference in expressions of the proteins related to grain filling in superior and inferior spikelets of two super rice cultivars, Liangyoupeijiu (indica hybrids) and Huaidao 9 (japonica) after anthesis through two-dimensional gel electrophoresis (SDS-PAGE) and the MALDI analysis. Results showed that the grain filling rate at 3–15 d after anthesis (DAA) and the final grain weight of inferior spikelets were much smaller than those of superior spikelets. The spots of protein expressed were fewer in inferior spikelets than in superior spikelets at 3, 8, and 15 DAA, and the results were reversed at 25 DAA. The spots of protein with two-fold expressions between superior and inferior spikelets showed a similar changing tendency. Twenty seven protein spots with significant difference in the expression between superior and inferior spikelets were chosen for the MALDI analysis, of which 14 protein spots were identified and their functions were analyzed. These proteins were involved in grain starch synthesis, protein synthesis, photosynthesis, energy metabolism, environmental adaptation and cell signal transduction. The results suggest that expression differences of the proteins related to grain filling account for the variation in grain filling between superior and inferior spikelets of rice.

Key words: Super rice, Superior/inferior spikelets, Expressions of the proteins related to grain filling, Protein function, Grain filling

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