Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2178-2184.doi: 10.3724/SP.J.1006.2012.02178

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of Wheat Lines with Resistance to Take-All and Powdery Mildew Diseases

ZHU Xiu-Liang1,LI Zhao1,DU Li-Pu1,XU Hui-Jun1,YANG Li-Hua1,2,ZHUANG Hong-Tao1,MA Ling-Jian2,ZHANG Zeng-Yan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetic and Breeding of Agriculture Ministry / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2012-04-25 Revised:2012-07-05 Online:2012-12-12 Published:2012-09-10
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn, Tel: 010-82108781

Abstract:

“Take-all”, primarily caused by Gaeumannomyces graminis var. tritici (Ggt), and powdery mildew, mainly caused by Blumeria graminis f. sp. tritici (Bgt), are important diseases of wheat (Triticum aestivum L.) worldwide. The wheat cultivar Yangmai 18 carrying a powdery mildewresistance gene Pm21, shows broad-spectrum resistance to powdery mildew. We have isolated a lipid transfer protein gene TaLTP5 from wheat. To study the role of TaLTP5 in wheat defense responses to the major pathogens of take-all, we introduced this gene intowheat cultivar Yangmai 18via bombarding the particle containing the TaLTP5 expressing vector pA25-TaLTP5. The TaLTP5 transgenic wheat plants from T0 to T3 generations were subjected to PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. We also evaluated the disease resistances of these TaLTP5 transgenic plants against inoculating Ggt and Bgt. The PCR and Southern blotting results showed that the alien TaLTP5 was transferred and integrated into the genomes of three transgenic wheat lines, and inherited stably in the transgenic wheat lines. The RT-PCR and Q-RT-PCR results indicated that the introduced TaLTP5 was over-expressed in transgenic wheat lines, which showed significantly-enhanced resistance to take-all, suggesting that TaLTP5 gene is involved in defense response to Ggt infection. In addition, the resistance of transgenic lines to powdery mildew was not influenced bythe introduced gene TaLTP5. Thus, TaLTP5 transgenic wheat Yangmai 18 exhibits resistance to both “Take-all” and powdery mildew.

Key words: Wheat lipid transfer protein, TaLTP5, Transgenic wheat, Take-all, Powdery mildew, Resistance

[1]Dong J-L(董建力), Hui H-X(惠红霞), Huang L-L(黄丽丽), Wang J-D(王敬东), Zhu Y-X(朱永兴), Chen X(陈孝), Ye X-G(叶兴国). Optimization of identification technique and germplasm screening of resistance to take-all in wheat. J Northwest A&F Univ (Nat Sci Edn) (西北农林科技大学学报•自然科学版), 2009, 37(3): 159–162 (in Chinese with English abstract)



[2]Huo Z-G(霍治国), Chen L(陈林), Liu W-G(刘万才), Xue C-Y(薛昌颖), Zhao S-J(赵圣菊), Zhuang L-W(庄立伟). Climatic zonation of wheat powdery mildew in china. Acta Ecol Sin (生态学报), 2002, 22(11): 1873-1881 (in Chinese with English abstract)



[3]Zhang B-Q(张伯桥). Utilizing rolling convergent backcross method to breed new wheat variety Yangmai 18 with powdery mildew resistance. Chin Agric Sci Bull (中国农学通报), 2009, 25(13): 74–77 (in Chinese with English abstract)



[4]Van Loon L C, van Strien E A. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol Mol Plant Pathol, 1999, 55: 85–97



[5]Kader J C. Lipid-transfer protein in pants. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47: 627–654



[6]Maldonado A M, Doerner P, Dixon R A, Lamb C J, Cameron R K. A putative lipid transfer protein involved in systemic resistance signaling in Arabidopsis. Nature, 2002, 419: 399–403



[7]Roy-Barman S, Sautter C, Chattoo B B. Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses. Transgenic Res, 2006, 15: 435–446



[8]Sujon S, Young J K, Ki D K, Byung K H, Sung H O, Jeong S S. Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco. Plant Cell Rep, 2009, 28: 419–427



[9]King G J, Turner V A, Hussey C E, Wurtele E S, Lee S M. Isolation and characterization of a tomato cDNA clone which codes for a salt-induced protein. Plant Mol Biol, 1988, 10: 401–412



[10]White A J, Dunn M A, Brown K, Hughes M A. Comparative analysis of genomic sequence and expression of a lipid transfer protein gene family in winter barley. J Exp Bot, 1994, 45: 1885–1892



[11]Sterk P, Booij H, Schellkens G A, van Kammen A, de Vries S C. Cell-specific expression of the carrot EP-2 lipid transfer protein gene. Plant Cell, 1991, 3: 907–921



[12]Kirubakaran S I, Begum S M, Ulaganathan K, Sakthivel K. Characterization of a new antifungal lipid transfer protein from wheat. Plant Physiol Biochem, 2008, 46: 918–927



[13]Sun J Y, Gaudet D A, Lu Z X, Frick M, Puchalski B, Laroche A. Characterization and antifungal properties of wheat nonspecific lipid transfer proteins. Mol Plant-Microbe Interact, 2008, 21: 346–360



[14]Xu H-J(徐惠君), Pang J-L(庞俊兰), Ye X-G(叶兴国), Du L-P(杜丽璞), Li L-C(李连城), Xin Z-Y(辛志勇), Ma Y-Z(马有志), Chen J-P(陈剑平), Chen J(陈炯), Cheng S-H(程顺和), Wu H-Y(吴宏亚). Study on the gene transferring of Nib8 into wheat for its resistance to the Yellow mosaic virus by bombardment. Acta Agron Sin (作物学报), 2001, 27(6): 684−689 (in Chinese with English abstract)



[15]Sharp P J, Kries M, Shewry P R, Gale M D. Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet, 1988, 75: 286–290



[16]Cao A Z, Wang X E, Chen Y P, Zou X W, Chen P D. A sequence-specific PCR marker linked with Pm21 distinguishes chromosomes 6AS, 6BS, 6DS of Triticum aestivum and 6VS of Haynaldia villosa. Plant Breed, 2006, 125: 201−205



[17]Zhang Z-Y(张增艳), Xu J-S(许景升), Liu Y-G(刘耀光), Wang X-P(王晓萍), Lin Z-S(林志珊), Xin Z-Y(辛志勇). Isolation of resistance gene candidates by a resistance gene analog of Thinopymm intermedium and pooled-PCR. Acta Agron Sin (作物学报), 2004, 30(3): 189–195 (in Chinese with English abstract)



[18]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 2001, 25: 402−408



[19]Liu Z Y, Sun Q X, Ni Z F, Yang T M. Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat. Plant Breeding, 1999, 118: 215−219



[20]Zhu X L, Li Z, Xu H J, Zhou M P, Du L P, Zhang Z Y. Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat. Funct Integr Genomic, DOI: 10.1007/s10142-012-0286-z (online)



[21]Cook R J, Take-all of wheat. Physiol Mol Plant Pathol, 2003, 62: 73–86

[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[3] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[4] WANG Xing-Rong, LI Yue, ZHANG Yan-Jun, LI Yong-Sheng, WANG Jun-Cheng, XU Yin-Ping, QI Xu-Sheng. Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage [J]. Acta Agronomica Sinica, 2022, 48(5): 1279-1287.
[5] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[6] SHI Yu-Qin, SUN Meng-Dan, CHEN Fan, CHENG Hong-Tao, HU Xue-Zhi, FU Li, HU Qiong, MEI De-Sheng, LI Chao. Genome editing of BnMLO6 gene by CRISPR/Cas9 for the improvement of disease resistance in Brassica napus L [J]. Acta Agronomica Sinica, 2022, 48(4): 801-811.
[7] LIU Dan, ZHOU Cai-E, WANG Xiao-Ting, WU Qi-Meng, ZHANG Xu, WANG Qi-Lin, ZENG Qing-Dong, KANG Zhen-Sheng, HAN De-Jun, WU Jian-Hui. Rapid identification of adult plant wheat stripe rust resistance gene YrC271 using high-throughput SNP array-based bulked segregant analysis [J]. Acta Agronomica Sinica, 2022, 48(3): 553-564.
[8] YANG Xin, LIN Wen-Zhong, CHEN Si-Yuan, DU Zhen-Guo, LIN Jie, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Li-Wu. Molecular identification of a geminivirus CoYVV and screening of resistant germplasms in jute [J]. Acta Agronomica Sinica, 2022, 48(3): 624-634.
[9] ZHANG Si-Meng, NI Wen-Rong, LYU Zun-Fu, LIN Yan, LIN Li-Zhuo, ZHONG Zi-Yu, CUI Peng, LU Guo-Quan. Identification and index screening of soft rot resistance at harvest stage in sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(8): 1450-1459.
[10] FU Hua-Ying, ZHANG Ting, PENG Wen-Jing, DUAN Yao-Yao, XU Zhe-Xin, LIN Yi-Hua, GAO San-Ji. Identification of resistance to leaf scald in newly released sugarcane varieties at seedling stage by artificial inoculation [J]. Acta Agronomica Sinica, 2021, 47(8): 1531-1539.
[11] XI Ling, WANG Yu-Qi, ZHU Wei, WANG Yi, CHEN Guo-Yue, PU Zong-Jun, ZHOU Yong-Hong, KANG Hou-Yang. Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province [J]. Acta Agronomica Sinica, 2021, 47(7): 1309-1323.
[12] ZUO Xiang-Jun, FANG Peng-Peng, LI Jia-Na, QIAN Wei, MEI Jia-Qin. Characterization of aphid-resistance of a hairy wild Brassica oleracea taxa, B. incana [J]. Acta Agronomica Sinica, 2021, 47(6): 1109-1113.
[13] MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping. Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton [J]. Acta Agronomica Sinica, 2021, 47(6): 1197-1202.
[14] ZHAO Jia-Jia, QIAO Ling, WU Bang-Bang, GE Chuan, QIAO Lin-Yi, ZHANG Shu-Wei, YAN Su-Xian, ZHENG Xing-Wei, ZHENG Jun. Seedling root characteristics and drought resistance of wheat in Shanxi province [J]. Acta Agronomica Sinica, 2021, 47(4): 714-727.
[15] ZHENG Ying-Xia, CHEN Du, WEI Peng-Cheng, LU Ping, YANG Jin-Yue, LUO Shang-Ke, YE Kai-Mei, SONG Bi. Effects of planting density on lodging resistance and grain yield of spring maize stalks in Guizhou province [J]. Acta Agronomica Sinica, 2021, 47(4): 738-751.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!