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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1739-1745.doi: 10.3724/SP.J.1006.2013.01739

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of an Adenylate Kinase Gene GmADK in Soybean

GAI Jiang-Tao,ZHAO Tuan-Jie,LI Yan*,GAI Jun-Yi*   

  1. Soybean Research Institute / National Center for Soybean Improvement / MOA Key Laboratory for Biology and Genetic Improvement of Soybean (General) / National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2013-01-18 Revised:2013-05-24 Online:2013-10-12 Published:2013-07-31
  • Contact: 李艳, E-mail: yanli1@njau.edu.cn; 盖钧镒, E-mail: sir@njau.edu.cn

Abstract:

Adenylate kinase (ADK) is an important enzyme in cellular energy homeostasis, which catalyzes the interconversion of adenine nucleotides, ATP+AMP⇔2ADP, and is involved in many processes including plant growth, development and response to abiotic stresses. To date, there has no report on cloning and expression analysis of soybean ADK gene yet. In this study, a soybean ADK gene was cloned from the leaves of a salt tolerant cultivar NN1138-2 using RT-PCR, and was designated as GmADK. The coding sequence (CDS) of this cloned ADK gene is 804 bp in length, encoding a polypeptide of 267 amino acids. Its protein was predicted to be located in plastids, containing a typical ATP-AMP (Ap5A) binding site and an AMP binding site. Multiple sequence alignments and phytogenetic analysis of ADK proteins showed GmADK is most similar with ADK from Phaseolus vulgarisand Medicago truncatula. Tissue expression pattern of GmADK showed its mRNA was more abundant in soybean roots and leaves than in stems. Quantitative RT-PCR showed a differential expression pattern of GmADK in response to salt between salt tolerant (NN1138-2) and sensitive (Kefeng-1) soybean genotypes. Compared with untreated plants, the expression of GmADK in both leaves and roots was repressed after 6, 12, and 24 hours of 200 mmol L–1 NaCl treatment in the salt sensitive cultivar, but induced in the salt tolerant cultivar, indicating that GmADK might be involved in soybean response to salt stress.

Key words: Soybean (Glycine max [L.] Merr.), Adenylate kinase (ADK), Gene cloning, Salt stress

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