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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1727-1738.doi: 10.3724/SP.J.1006.2013.01727

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Sequence Diversity of ZmLEC1 and Association Analysis of Embryogenic calli Formation Ability in Maize

LI Zhao1,2,ZHANG Deng-Feng2,SUN Yong-Hua2,WU Xun2,LI Yong-Xiang2,SHI Yun-Su2,SONG Yan-Chun2,YANG De-Guang1,*,WANG Tian-Yu2,LI Yu2,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China;2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-28 Revised:2013-06-02 Online:2013-10-12 Published:2013-08-01
  • Contact: 杨德光, E-mail: ydgl@tom.com; 黎裕, E-mail: liyu03@caas.cn, Tel: 010-62131196

Abstract:

A maize association mapping population consisted of a mini core collection of ninety-five maize inbred lines and three elite maize accessions (A188, HiII, and Zong 31)usually for genetic transformation was used to analyze the sequence diversity and linkage disequilibrium (LD) of ZmLEC1, a candidate gene of regeneration ability in maize. A candidate gene association strategy was used to reveal the relationship between this gene and embryogenic calli formation ability and discover favorable alleles and genotypes enhancing the embryogenic calli formation ability. The results showed that there existed significant differences in abilities of embryogenic calli formation and regeneration among these accessions. The calli induced from Yue267-1-1 were very similar to those of HiII, the popularly used genotype in maize transformation. Yue267-1-1 had the highest ability of embryogenic calli formation and regeneration and could be a new germplasm for immature embryo-based genetic transformation. The result of sequence polymorphism analysis of ZmLEC1 showed that there were thirty-three SNPs and nine InDels in the coding region of 852 bps. The LD between all of the informative polymorphisms decayed rapidly to about 300 bp at R2=0.1. Totally four polymorphic sites in the ZmLEC1 gene were significantly associated with embryogenetic calli formation ability.

Key words: Zea mays, ZmLEC1, Embryogenetic callus, Association analysis

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