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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1720-1726.doi: 10.3724/SP.J.1006.2013.01720

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Interaction between Wheat Resistance-related Kinase TiDPK1 and BYDV Coat Protein

WANG Xin-Dong,CHEN Liang,ZHANG Zeng-Yan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-02-05 Revised:2013-04-22 Online:2013-10-12 Published:2013-08-01
  • Contact: 张增艳, E-mail: zhangzengyan@ caas.cn, Tel: 010-82108781

Abstract:

Yellow dwarf virus disease is one of the important diseases of wheat (Tritium aestivum L.) worldwide. It is caused by Barley yellow dwarf virus (BYDV) that is vectored by aphids. A kinase protein encoding gene TiDPK1, which is derived from Thinopyrum intermedium, is an important gene involved in BYDV resistance in wheat-T. intermedium translocation lines. In this study, we used yeast two-hybrid and bimolecular fluorescence complementation assays to explore the relationship between TiDPK1 and coat protein (CP) of BYDV (BYDV-CP). The results proved that the protein TiDPK1 interacted with BYDV-CP, which may offer an insight to the resistance mechanism of TiDPK1.

Key words: Wheat, Protein kinase TiDPK1, Coat protein of Barley yellow dwarf virus, Yeast two-hybrid, Bimolecular fluorescence complementation, Protein–protein interaction

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