超高产夏玉米花粒期不同部位叶片衰老与抗氧化酶特性

doi:10.3724/SP.J.1006.2013.02183

Abstract

Abstract:

Grain yield improvement of maize (Zea mays L.) has been evidently associated with delayed leaf senescence during the grain-filling period after anthesis. The object of this study was to explore senescence characteristics and antioxidant enzyme activities after flowering in different position leaves of summer maize with high yield record for providing references of regulating the leaf senescence and achieving the higher yield in China. A field experiment planted Denghai 661, a new hybrid with high-yielding potential, was carried out to compare the experiment of maize with high yield record (EHYR) and the maize grown in conventional farmers’ field (MCFF) in individual level from 2005 to 2007. And the parameters related to the leaf senescence, such as leaf area (LA), LA reduction, net photosynthetic rate (P_n), soluble protein content, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and malondialdehyde (MDA) content, were determined in this research. The grain yield of EHYR was 2.18 times greater than that of MCFF, reaching 19 349 kg ha^-1. The rapid senescence time of EHYR leaves and MCFF leaves were found at 30 and 50 days after anthesis, respectively. Therefore, the leaf senescence of EHYR was 20 days earlier than that of MCFF. At the rapid senescence stage, the leaf area reduction of EHYR was 5.7% lower than that of MCFF. During the later period of leaf senescence, the net photosynthetic rate was higher in top and middle leaves than in bottom leaves. Furthermore, the soluble protein content in leaves of EHYR was higher than that of MCFF, but the MDA content was on the contrary. At 20 days after anthesis, the SOD activities in top and middle leaves of EHYR were higher than those of MCFF, while the SOD, POD, and CAT activities in bottom leaves of EHYR were higher than those of MCFF. On the contrary, the POD and CAT activities in middle leaves of MCFF were higher than those of EHYR. Results of both correlation analysis and path analysis indicated that the leaf reduction was negatively correlated with the CAT activity significantly in EHYR, also with the SOD and POD activities significantly in MCFF, and the direct effects were higher than the indirect effects of SOD, POD, and CAT in both EHYR and MCFF. Compared with MCFF treatment, besides higher SOD and POD activities, EHYR had higher CAT activities and soluble protein content resulting in declined lipid peroxidation during grain-filling period, which caused delayed leaf senescence and leaf senescence degree. There were obvious differences at 20 days post-anthesis in maize leaf senescence physiologically. Between EHYR and MCFF suggesting that the 20–30 days after anthesis is a critical period, and all the related agronomy measures should be recommended to be taken before this phase in maize production.

Key words: Summer maize, Super-high yielding potential, After anthesis, Leaf senescence, Antioxidant enzyme

WANG Yong-Jun,YANG Jin-Sheng,YUAN Cui-Ping,LIU Jing-Guo,LI Deng-Hai,DONG Shu-Ting. Characteristics of Senescence and Antioxidant Enzyme Activities in Leaves at Different Plant Parts of Summer Maize with the Super-high Yielding Potential after Anthesis[J].Acta Agron Sin, 2013, 39(12): 2183-2191.

References

[1]Gardner F P, Pearce R B, Mitchell R L. Physiology of Crop Plant. Ames: Iowa State University Press, 1985. pp 3–30

[2]Li S-K(李少昆), Wang C-T(王崇桃). Potential and Ways to High Yield in Maize (玉米高产潜力途径). Beijing: Science Press, 2010. pp 217–354 (in Chinese)

[3]Tollenaar M, Daynard T B. Leaf senescence in short season maize hybrids. Can J Plant Sci, 1978, 58: 869–874

[4]Valentinuz O R, Tollenaar M. Vertical profile of leaf senescence during the grain-filling period in older and newer maize hybrids. Crop Sci, 2004, 44: 827–834

[5]Rajcana I, Dwyerb L M, Tollenaara M. Note on relationship between leaf soluble carbohydrate and chlorophyll concentrations in maize during leaf senescence. Field Crops Res, 1999, 63: 13–17

[6]Feller U K, Soong T S, Hageman R H. Leaf proteolytic activities and senescence during grain development of field-grown corn (Zea mays L.). Plant Physiol, 1977, 59: 290–294

[7]Gan S, Amasino R M. Inhibition of leaf senescence by autoregulated production of cytokinin. Science, 1995, 270: 1986–1988

[8]Guo Y, Gan S. Leaf senescence: signals, execution, and regulation. Curr Top Dev Biol, 2005, 71: 83–112

[9]Lim P O, Kim H J, Nam H G. Leaf senescence. Annu Rev Plant Biol, 2007, 58: 115–136

[10]Pastori G M, IHppi V S. Antioxidative protection in a drought-resistant maize strain during leaf senescence. Physiol Plant, 1993, S7: 227–231

[11]Hodges D M, Andrews C J, Johnson D A, Hamilton R I. Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines. J Exp Bot, 1997, 48(310): 1105–1113

[12]Colomb B, Kiniry J R, Debaeke P. Effect of soil phosphorus on leaf development and senescence dynamics of field-grown maize. Agron J, 2000, 92: 428–435

[13]Borrás L, Maddonni G A, Otegui M E. Leaf senescence in maize hybrids: plant population, row spacing and kernel set effects. Field Crops Res, 2003, 82: 13–26

[14]Erley G S, Begum N, Worku M, Bänziger M, Horst W J. Leaf senescence induced by nitrogen deficiency as indicator of genotypic differences in nitrogen efficiency in tropical maize. J Plant Nutr Soil Sci, 2007, 170: 106–114

[15]Hu T, Yuan L, Wang J, Kang S, Li F. Antioxidation responses of maize roots and leaves to partial root-zone irrigation. Agr Water Manage, 2010, 98: 164–171

[16]Zhang R-H(张仁和), Zheng Y-J(郑友军), Ma G-S(马国胜), Zhang X-H(张兴华), Lu H-D(路海东), Shi J-T(史俊通), Xue J-Q(薛吉全). Effects of drought stress on photosynthetic traits and protective enzyme activity in maize seeding. Acta Ecol Sin (生态学报), 2011, 31(5): 1303–1311 (in Chinese with English abstract)

[17]Stoddart J L, Thomas H. Leaf senescence. In: Boulter D, Parthier B, eds. Encyclopedia of Plant Physiology. Berlin: Springer Verlag, 1982. pp 592–636

[18]Betania F Q, Yoo-Sun N. Molecular aspects of leaf senescence. Trends Plant Sci, 2000, 5: 278–282

[19]Buchanan-Wollaston V. The molecular biology of leaf senescence. J Exp Bot, 1997, 48: 181–1991

[20]Dong S-T(董树亭). Eco-Physiology and Formation of Yield and Quality in Maize (玉米生态生理与产量品质形成). Beijing: Higher Education Press, 2006. pp 3–43, 49–113, 347–416 (in Chinese)

[21]Fageria N K, Baligar V C, Clark R B. Physiology of Crop Production. New York, London, Oxford: Food Products Press, 2005. pp 72–82

[22]Bradford M M. A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem, 1976, 72: 248–254

[23]Giannopolitis C N, Ries S K. Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol, 1977, 59: 309–314

[24]Hernandez J A, Jimenez A, Mullineaux P, Sevilla F. Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defenses. Plant Cell Environ, 2000, 23: 853–862

[25]Samantary S. Biochemical responses of Cr-tolerant and Cr-sensitive mung bean cultivars grown on varying levels of chromium. Chemosphere, 2002, 47: 1065–1072

[26]Heath R L, Packer L. Photoperoxidation in isolated chloroplast: I. Kinetics and stoichiometry of fatty acids peroxidation. Arch Biochem Biophys, 1968, 125: 189–198

[27]Wang Q-C(王庆成), Liu K-C(刘开昌). Theory and practices of high-yielding maize cultivation in Shandong. J Maize Sci (玉米科学), 2004, 12(suppl-2): 60–62 (in Chinese)

[28]He P(何萍), Jin J-Y(金继运). Effect of N and K nutrition on changes of endogenous hormone and metabolism of active oxygen during leaf senescence in spring maize. Plant Nutr Fert Sci (植物营养与肥料学报), 1999, 5(4): 289–296 (in Chinese with English abstract)

[29]Crafts-Brandner S J, Poneleit C G. Effect of ear removal on CO2 exchange and activities of ribulose bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase of maize hybrids and inbred lines. Plant Physiol, 1987, 84: 261–265

[30]Wang Y-J(王永军). Study on Population Quality and Individual Physiology Function of Super High-Yielding Maize (Zea mays L.). PhD Dissertation of Shandong Agricultural University, 2008 (in Chinese with English abstract)

[31]Huang Z-X(黄振喜), Wang Y-J(王永军), Wang K-J(王空军), Li D-H(李登海), Zhao M(赵明), Liu J-G(柳京国), Dong S-T(董树亭), Wang H-J(王洪军), Wang J-H(王军海), Yang J-S(杨今胜). Photosynthetic traits of over-15000 kg ha-1 summer maize hybrids during grain filling. Sci Agric Sin (中国农业科学), 2007, 40(9): 1898–1906 (in Chinese with English abstract)

[32]Fridovich I. Superoxide dismutases. Annu Rev Biochem, 1975, 44: 147–159

[33]Islam M R, Hu Y, Mao S, Jia P, Enejid A E, Xue X. Effects of water-saving superabsorbent polymer on antioxidant enzyme activities and lipid peroxidation in corn (Zea mays L.) under drought stress. J Sci Food Agric, 2011, 91: 813–819

[34]Ge T-D(葛体达), Sui F-G(隋方功), Bai L-P(白莉萍), Lü Y-Y(吕银燕), Zhou G-S(周广胜). Effects of water stress on the protective enzyme activities and lipid peroxidation in roots and leaves of summer maize. Sci Agric Sin (中国农业科学), 2005, 38 (5): 922–928 (in Chinese with English abstract)

[35]Shao G-Q(邵国庆), Li Z-J(李增嘉), Ning T-Y(宁堂原), Jiang B-J(蒋保娟), Jiao N-Y(焦念元). Effects of irrigation and urea types on ear leaf senescence after anthesis, yield and economic benefit of maize. Sci Agric Sin (中国农业科学), 2009, 42(10): 3459–3466 (in Chinese with English abstract)

[36]Nie L-X(聂乐兴), Jiang X-Y(姜兴印), Wu S-H(吴淑华), Zhang J-W(张吉旺), Liu P(刘鹏). Effects of DA-6 on leaf photosynthetic carboxylase and protective enzyme activities and grain yield of high-yielding summer maize. Chin J Appl Ecol (应用生态学报), 2010, 21(10): 2558–2564 (in Chinese with English abstract)

[37]Wang K-J(王空军), Hu C-H(胡昌浩), Dong S-T(董树亭), Liu K-C(刘开昌), Sun Q-Q(孙庆泉). Changes of the protective enzyme activities and lipid peroxidation after anthesis among maize varieties planted in different years. Acta Agron Sin (作物学报), 1999, 25(6): 700–706 (in Chinese with English abstract)

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