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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (02): 222-230.doi: 10.3724/SP.J.1006.2014.00222

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of Molecular Genetic Linkage Map of Sugarbeet

WANG Mao-Qian1,2,3,LI Bo1,WANG Hua-Zhong1,2,3,*   

  1. 1 Key Laboratory of Sugar Beet Genetic Breeding/ Crop Academy of Heilongjiang University, Harbin150080, China; 2 Sugar Beet Research Institute, Chinese Academy of Agricultural Sciences, Harbin150080, China; 3 Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences,Harbin 150080, China
  • Received:2013-03-11 Revised:2013-09-06 Online:2014-02-12 Published:2013-11-14
  • Contact: 王华忠, E-mail: wwhhzz0451@sohu.com, Tel: 0451-86609494

Abstract:

Molecular genetic map is a fundamental tool for genomic research. In this study, a molecular genetic map was constructed using an F2 population consisting of 200 individuals from a cross between sugarbeet JV34-2 (the high beet yield and low sugar content line) and sugarbeet 2B023 (the low beet yield and high sugar content line) and a total of 561 SRAP primers and 114 SSR primers. Approximately 56 out of 561 and 20 out of 114 primers, respectively, showed polymorphisms. Each of these polymorphic primers produced at least one scorable polymorphic DNA band which was visible enough for detection and scoring. The map consisted of nine linkage groups which included 141 (123 SRAP and 18 SSR) markers, and covered 1399.88 cM with an average distance of 9.92 cM. Three markers were still unlinked. All nine linkage groups consisting of 3–26 markers were 15.69–237.21 cM in length. Twenty point five six percent partially segregated markers distributed in the map, were mainly located on Ch3 linkage groups, and the others were mapped on Ch1, Ch2, Ch8, and Ch9. This is the first gene map of sugarbeet, constructed by SRAP and SSR markers in China. It will provide the basis for gene mapping of important characters and cloning of excellent gene in sugarbeet.

Key words: Sugarbeet, SRAP, SSR, Genetic linkage map

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