Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (03): 405-415.doi: 10.3724/SP.J.1006.2014.00405

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Characterization of Peanut Gene AhSOS2

ZHANG Guo-Jia1,2,HOU Lei2,WANG Qing-Guo2,LI Zhen2,DAI Shao-Jun1,LIU Wei2,*   

  1. 1Alkali Soil Natural Environmental Science Center, Northeast Forestry University / Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China; 2 Biotechnology Research Center, Shandong Academy of Agricultural Sciences / Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Shandong Province, Jinan 250100, China
  • Received:2013-06-20 Revised:2013-12-14 Online:2014-03-12 Published:2014-01-16
  • Contact: 刘炜, E-mail: wheiliu@163.com, Tel: 0531-83179572 E-mail:zhangguojia314@163.com

Abstract:

SOS2 (Salt Overly Sensitive 2) is a kind of important salt tolerance genes, which is involved in mediating the intracellular ion balance and plays an important role in the response of plants to salt damage and adaptation. In this study, a 1462 bp full-length cDNA, including a 1341 bp open reading frame, was isolated and harvested by RACE method. As the gene showed more than 70% homologous to members of SOS2 subfamily in other plants by bioinformatics analysis, it is then named as AhSOS2 (GenBank accession numberHG797656). Its coding protein AhSOS2 has 446 amino acids, and is a member of serine/threonine protein kinases. Quantitative real-time PCR (qRT-PCR) analysis showed that AhSOS2 was expressed constitutively in peanut tissues, and could be induced to express higher under salt and drought treatments. When treated with 250 mmol L-1 NaCl, the gene expression level increased dramatically to about 30 times higher than that of control in the stems of peanut seedlings, while the expression level of AhSOS2 increased markedly in the leaves of peanut seedlings when treated by drought stress that imitated by 30% PEG-6000.AhSOS2 is supposed to participate in stress resistance and tolerance in peanut. The expression binary vector of pCAMBIA1301P-AhSOS2 was constructed and the transgenic rice plants were obtained. The primary functional verification showed that the ability of salt tolerance and adaptation was enhanced in AhSOS2 overexpression rice. The above research is expected to uncover the adversity defense mechanisms of peanut grown under stress conditions, and further guide the resistance breeding and quality improvement of peanut.

Key words: Peanut, SOS2 gene AhSOS2, Quantitative real-time PCR, Expression pattern, Stress resistance, Quality improvement

[1]Greenway H, Munns R. Mechanisms of salt tolerance in nonhalophytes. Annu Rev Plant Physiol, 1980, 31: 149–190



[2]Zhu J K. Plant salt tolerance. Trends Plant Sci, 2001, 6: 66–71



[3]Bohnert H J, Nelson D E, Jensen R G. Adaptation to environmental stresses. Plant Cell, 1995, 7: 1099–1111



[4]Katiyar-Agarwal S, Zhu J, Kim K, Agarwal M, Fu X, Huang A, Zhu J K. The plasmamembrane Na+/H+ antiporter SOS1 interacts with RCD1 and functions in oxidative stress tolerance in Arabidopsis. Proc Natl Acad Sci USA, 2006, 103: 18816–18821



[5]Ji H, Pardo J M, Batelli G, Van Oosten M J, Bressan R A, Li X. The salt overly sensitive (SOS) pathway: established and emerging roles. Mol Plant, 2013, 6: 275–186



[6]Shi H, Pardo Q, Zhu J K. The putative plasma membrane Na+/H+ antiporter SOS1 controls long distance Na+ transport in plants. Plant Cell, 2002, 14: 465–477



[7]Gong D, Guo Y, Schumaker K S, Zhu J K. The SOS3 family of calcium sensors and SOS2 family of protein kinases in Arabidopsis. Plant Physiol, 2004, 134: 919–926



[8]Wu S J, Ding L, Zhu J K. SOS1, a genetic locus essential for salt tolerance and potassium acquisition. Plant Cell, 1996, 8: 617–627



[9]Liu J, Ishitani M, Halfter U, Kim C S, Zhu J K. The arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance. Proc Natl Acad Sci USA, 2000, 97: 3735–3740



[10]Shi H, Zhu J K. SOS4, a pyridoxal kinase gene, is required for root hair development in arabidopsis. Plant Physiol, 2002, 129: 585–593



[11]Shi H, Kin Y S, Guo Y, Stevenson B, Zhu J K. The Arabidopsis SOS5 locus encodes a putative cell surface adhesion protein and required for normal cell expansion. Plant Cell, 2003, 15: 19–32



[12]Gong D, Guo Y, Jagendorf A T, Zhu J K. Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance. Plant Physiol, 2002, 130: 256–264



[13]Guo Y, Halfter U, Ishitani M, Zhu J K. Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance. Plant Cell, 2001, 13: 1383–1399



[14]Liu J, Halfter U, Kim C S, Shi W, Zhu J K. SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. Proc Natl Acad Sci USA, 2000, 97: 3730–3734



[15]Qiu Q S, Guo Y, Dietrich M A, Schumaker K S, Zhu J K. Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc Natl Acad Sci USA, 2002, 99: 8436–8441



[16]Hu D G, Li M, Luo H, Dong Q L, Yao Y X, You C X, Hao Y J. Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis. Plant Cell, 2012, 31: 713–722



[17]Quan R, Lin H, Mendoza I, Zhang Y, Cao W, Yang Y, Shang M, Chen S, Pardo J M, Guo Y. SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect arabidopsis shoots from salt stress. Plant Cell, 2007, 19: 1415–1431



[18]张吉民, 苗华荣, 李正超, 郝素美, 闫强. 花生加工利用、贸易现状与展望. 武汉工业学院学报, 2002, (2): 104–106



Zhang J M, Miao H R, Li Z C, Hao S M , Yan Q, Zhu E J. Status and prospectsfor the processing, utilization and trade of peanut. Wuhan Polytech Univ, 2002, (2): 104–106 (in Chinese with English abstract)



[19]Moretzsohn Mde C, Hopkins M S, Mitchell S E, Kresovich S, Valls J F, Ferreira M E. Genetic diversity of Peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol, 2004, 4: 11



[20]姜慧芳, 任小平. 干旱胁迫对花生叶片SOD 活性和蛋白质的影响. 作物学报, 2004, 30: 169–174



Jing H F, Ren X P. Effect on SOD activity and protein content in groundnut leaves by drought stress. Acta Agron Sin, 2004, 30: 169–174 (in Chinese with English abstract)



[21]Bi Y P, Liu W, Xia H, Su L, Zhao C Z, Wan S B, Wang X J. EST sequencing and gene expression profiling of cultivated peanut (Arachis hypogaea L.). Genome, 2010, 53: 832–839



[22]Huang C, Picimbona J F, Li H Q, Li Z, Liu, Q, Liu W. An efficient method for total RNA extraction from peanut seeds. Russ J Plant Physiol, 2012, 59: 129–133



[23]Liu W, Xu Z H, Luo D, Xue H W. OsCKI1, a rice casein kinase I, plays significant roles in auxin related root development and functions of plant hormones. Plant J, 2003, 36, 189–202



[24]Hofgen R, Willmitzer L. Storage of competent cell for agrobacterium transformation. Nucl Acids Res, 1988, 16: 9877



[25]刘巧泉, 张景六, 王宗阳, 洪孟民, 顾铭洪. 根癌农杆菌介导的水稻高效转化系统的建立. 植物生理学报, 1998, 24: 259–271



Liu Q Q, Zhang J L, Wang Z Y, Hong M M, Gu M H. A highly efficient transformation system mediated by Agrobacterium tumefaciens in rice (Oryza sativa L.). Plant Physiol Mol Biol, 1998, 24: 259–271 (in Chinese with English abstract)



[26]Jefferson R A. Assaying chimeric genes in plants: the GLJS gene fusion system. Plant Mol Biol Rep, 1987, 5: 387−405



[27]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321−4325



[28]Zhu J K. Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physion, 2000, 124: 941–948



[29]Chinnusamy V, Jagendorf A, Zhu J K. Understanding and improving salt tolerance in plants. Crop Sci, 2005, 45: 437–448



[30]Li L, Jia Z Q, Zhu Y J, Qi Y l. Research advances on drought resistance mechanism of plant species in arid area of China. J Desert Res, 2010, 30: 1053–1060



[31]Shi H, Ishitani M, Kim C, Zhu J K. The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. Proc Natl Acad Sci USA, 2000, 97: 6896–6901



[32]Ishitani M, Liu J, Halfter U, Kim C S, Shi W, Zhu J K. SOS3 function in plant salt tolerance requires N-myristoylation and calcium-binding. Plant Cell, 2000, 12: 1667–1677



[33]Zhu J K, Liu J, Xiong L. Genetic analysis of salt tolerance in Arabidopsis: evidence for a critical role of potassium nutrition. Plant Cell, 1998, 10, 1181–1191



[34]Liu J, Zhu J K. An Arabidopsis mutant that requires increased calcium for potassium nutrition and salt tolerance. Proc Natl Acad Sci USA, 1997, 94, 14960–14964



[35]Chen X F, Gu Z M, Liu F, Ma B J, Zhang H S. Molecular analysis of rice CIPKs involved in both biotic and abiotic stress responses. Rice Sci, 2011, 18: 1–9



[36]Halfter U, Ishitani M, Zhu J K. The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3. Proc Natl Acad Sci USA, 2000, 97: 3735–3740



[37]Jeong H J, Jwa N S, Kim K N. Identification and characterization of protein kinases that interact with the CBL3 calcium sensor in Arabidopsis. Plant Sci, 2005, 169: 1125–1135



[38]Hwang Y S, Bethke P C, Cheong Y H, Chang H S, Zhu T, Jones R L. A gibberellin-regulated calcineurin B in rice localizes to the tonoplast and is implicated in vacuole function. Plant Physiol, 2005, 138: 1347–1358



[39]Mahajan S, Sopory S K, Tuteja N. Cloning and characterization of CBL-CIPK signaling components from a legume (Pisum sativum). FEBS J, 2006, 273: 907–925



[40]Hrabak E M, Chan C W, Gribskow M, Harper J F, Choi J H, Halford N, Kudla J, Luan S, Nimmo H G, Sussman M R, Thomas M, Walker-Simmons K, Zhu J K, Harmon A C. Arabidopsis CDPK-SnRK super family of protein kinases. Plant Physiol, 2003, 132, 666–680



[41]Weinl S, Kudla J. The CBL-CIPK Ca2+-decoding signaling network: function and perspectives. New Phytol, 2009, 184: 517–528



[42]Chenk P W, Snaar-Jagalska B E. Signal perception and transduction: the role of protein kinases. Biochim Biophys Acta, 1999, 1449: 1–24



[43]Stone J M, Walker J C. Plant protein kinase families and signal transduction. Plant Physiol, 1995, 108: 451–457



[44]Pauly N, Knight M R, Thuleau P, vander Luit A H, Moreau M, Trewavas A J, Ranjeva R, Mazars C. Control of free calcium in plant cell nuclei. Nature, 2000, 405: 754–755



[45]Knight H. Calcium signaling during abiotic stress in plants. Int Rev Cytol, 1999, 195: 269–324



[46]Zhu J K, Liu J P, Xiong L M. Genetic analysis of salt tolerance in Arabidopsis thaliana: evidence of a critical role for potassium nutrition. Plant Cell, 1998, 10:1181–1192

 


 
[1] YANG Huan, ZHOU Ying, CHEN Ping, DU Qing, ZHENG Ben-Chuan, PU Tian, WEN Jing, YANG Wen-Yu, YONG Tai-Wen. Effects of nutrient uptake and utilization on yield of maize-legume strip intercropping system [J]. Acta Agronomica Sinica, 2022, 48(6): 1476-1487.
[2] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[3] YUAN Da-Shuang, DENG Wan-Yu, WANG Zhen, PENG Qian, ZHANG Xiao-Li, YAO Meng-Nan, MIAO Wen-Jie, ZHU Dong-Ming, LI Jia-Na, LIANG Ying. Cloning and functional analysis of BnMAPK2 gene in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(4): 840-850.
[4] HUANG Cheng, LIANG Xiao-Mei, DAI Cheng, WEN Jing, YI Bin, TU Jin-Xing, SHEN Jin-Xiong, FU Ting-Dong, MA Chao-Zhi. Genome wide analysis of BnAPs gene family in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(3): 597-607.
[5] DING Hong, XU Yang, ZHANG Guan-Chu, QIN Fei-Fei, DAI Liang-Xiang, ZHANG Zhi-Meng. Effects of drought at different growth stages and nitrogen application on nitrogen absorption and utilization in peanut [J]. Acta Agronomica Sinica, 2022, 48(3): 695-703.
[6] HUANG Li, CHEN Yu-Ning, LUO Huai-Yong, ZHOU Xiao-Jing, LIU Nian, CHEN Wei-Gang, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang. Advances of QTL mapping for seed size related traits in peanut [J]. Acta Agronomica Sinica, 2022, 48(2): 280-291.
[7] YU Hui-Fang, ZHANG Wei-Na, KANG Yi-Chen, FAN Yan-Ling, YANG Xin-Yu, SHI Ming-Fu, ZHANG Ru-Yan, ZHANG Jun-Lian, QIN Shu-Hao. Genome-wide identification and expression patterns in response to signals from Phytophthora infestans of CrRLK1Ls gene family in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 249-258.
[8] JIAN Hong-Ju, SHANG Li-Na, JIN Zhong-Hui, DING Yi, LI Yan, WANG Ji-Chun, HU Bai-Geng, Vadim Khassanov, LYU Dian-Qiu. Genome-wide identification and characterization of PIF genes and their response to high temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 86-98.
[9] WANG Ying, GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, LAI Hua-Jiang, PAN Xiao-Yi, BI Chen, LI Xiang-Dong, YANG Dong-Qing. Identification of gene co-expression modules of peanut main stem growth by WGCNA [J]. Acta Agronomica Sinica, 2021, 47(9): 1639-1653.
[10] WANG Jian-Guo, ZHANG Jia-Lei, GUO Feng, TANG Zhao-Hui, YANG Sha, PENG Zhen-Ying, MENG Jing-Jing, CUI Li, LI Xin-Guo, WAN Shu-Bo. Effects of interaction between calcium and nitrogen fertilizers on dry matter, nitrogen accumulation and distribution, and yield in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1666-1679.
[11] SHI Lei, MIAO Li-Juan, HUANG Bing-Yan, GAO Wei, ZHANG Zong-Xin, QI Fei-Yan, LIU Juan, DONG Wen-Zhao, ZHANG Xin-You. Characterization of the promoter and 5'-UTR intron in AhFAD2-1 genes from peanut and their responses to cold stress [J]. Acta Agronomica Sinica, 2021, 47(9): 1703-1711.
[12] GAO Fang, LIU Zhao-Xin, ZHAO Ji-Hao, WANG Ying, PAN Xiao-Yi, LAI Hua-Jiang, LI Xiang-Dong, YANG Dong-Qing. Source-sink characteristics and classification of peanut major cultivars in North China [J]. Acta Agronomica Sinica, 2021, 47(9): 1712-1723.
[13] ZHANG He, JIANG Chun-Ji, YIN Dong-Mei, DONG Jia-Le, REN Jing-Yao, ZHAO Xin-Hua, ZHONG Chao, WANG Xiao-Guang, YU Hai-Qiu. Establishment of comprehensive evaluation system for cold tolerance and screening of cold-tolerance germplasm in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1753-1767.
[14] XUE Xiao-Meng, WU JIE, WANG Xin, BAI Dong-Mei, HU Mei-Ling, YAN Li-Ying, CHEN Yu-Ning, KANG Yan-Ping, WANG Zhi-Hui, HUAI Dong-Xin, LEI Yong, LIAO Bo-Shou. Effects of cold stress on germination in peanut cultivars with normal and high content of oleic acid [J]. Acta Agronomica Sinica, 2021, 47(9): 1768-1778.
[15] HAO Xi, CUI Ya-Nan, ZHANG Jun, LIU Juan, ZANG Xiu-Wang, GAO Wei, LIU Bing, DONG Wen-Zhao, TANG Feng-Shou. Effects of hydrogen peroxide soaking on germination and physiological metabolism of seeds in peanut [J]. Acta Agronomica Sinica, 2021, 47(9): 1834-1840.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!