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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (04): 539-547.doi: 10.3724/SP.J.1006.2015.00539

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of Ghkinesin13 Subfamily Genes in Gossypium hirsutum

ZHAO Lan-Jie1,XUE Fei2,ZHU Shou-Hong2,LI Yan-Jun2,LIU Yong-Chang2,*,SUN Jie2   

  1. 1 College of Life Sciences, Shihezi University, Shihezi 832003, China; 2 Agricultural College of Shihezi University / The Key Laboratory of Oasis Eco-agriculture Xinjiang Production and Construction Group, Shihezi 832003, China
  • Received:2014-09-18 Revised:2015-02-06 Online:2015-04-12 Published:2015-03-02
  • Contact: 刘永昌, E-mail: liuyongchang2003@126.com, Tel: 18799292449 E-mail:lyj20022002@sina.com.cn

Abstract:

Kinesin family belongs to a class of motor proteins. Kinesins can move along microtubule filaments by using the energy released from ATP hydrolysis, and play key roles during cell formation and cell elongation. Using the protein sequence of Atkinesin13A as a probe, seven genes with high sequence homology were obtained from a genome database of Gossypium raimondii diploid cotton with Blast alignment. These sequences were used to design primers, and then seven genes were isolated from upland cotton fiberusing RT-PCR. Based on the homology level of the seven genes with Atkinesin13A and Atkinesin13B, we designated them as GhKIS13A1, GhKIS13A2, GhKIS13A3, GhKIS13B1, GhKIS13B2, GhKIS13B3, and GhKIS13B4 respectively. Bioinformatic analysis showed that seven proteins contained typical KISC domains including the central motor, ATP-binding sites and microtubule binding sites. Multiple sequence alignment and phylogenetic tree analysis revealed that seven genes can be divided into Kinesin13A and Kinesin13B. The qPCR showed that the seven Ghkinesin13 subfamily genes expressed in all tissues of cotton, but showed different expression patterns. Of the seven genes, only GhKIS13A4 was preferentially expressed in fibers, suggesting that it may play an important role in cotton fiber development. This study can provide a foundation for studying the functions of Ghkinesin13 subfamily genes in fiber development.

Key words: Cotton, Fiber, Kinesin, Expression analysis

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