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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (09): 1411-1416.doi: 10.3724/SP.J.1006.2016.01411

• RESEARCH NOTES • Previous Articles    

Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 101 Main Sugarcane Breeding Parents in China

LI Wen-Feng,WANG Xiao-Fan,HUANG Ying-Fun*,ZHANG Rong-Yue,SHAN Hong-Li,LUO Zhi-Ming,YIN Jiong   

  1. Sugarcane Research Institute, Yunnan Province Academy of Agricultural Sciences,, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China
  • Received:2016-03-17 Revised:2016-06-20 Online:2016-09-12 Published:2016-06-27
  • Contact: 黄应昆, E-mail: huangyk64@163.com, Tel: 0873-7227017 E-mail:ynlwf@163.com
  • Supported by:

    This study was supported by China Agriculture Research System (CARS-20-2-2) and the Agriculture Research System of Yunnan Province.

Abstract:

Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Bru1 is a major brown rust resistance gene with a broad-spectrum resistance against various brown rust pathogens originating from different countries. In order to assess the resistance level of main sugarcane breeding parents to Puccinia melanocephala, and determine the distribution of the Bru1 gene in these breeding parents in China, 101 main sugarcane breeding parents collected from the Chinese National Nursery of Sugarcane Germplasm Resources were evaluated and identified for their brown rust resistance at the seedling stage and for the presence of the brown rust resistance gene Bru1. Bru1 was detected to present in 48 (47.5%) resistant clones out of the 101 tested parental lines, indicating that brown rust resistance in main sugarcane breeding parents in China is primarily controlled by Bru1. The absence of Bru1 in the other 29 resistant and 24 susceptible parental lines suggests that they may carry brown rust resistance-associated genes other than Bru1. These results may facilitate future efforts to breed varieties with improved resistance to brown rust and may provide resistant parental lines for selecting and planting elite resistant varieties to effectively control brown rust disease in sugarcane.

Key words: Sugarcane, Main breeding parents, Brown rust resistance gene Bru1, Molecular detection.

[1]Ryan C C, Egan B T. Rust, Chap. XIII. In: Ricaud C, Egan B T, Gillaspie A G, Hughes C G, eds. Diseases of Sugarcane: Major Diseases. Amsterdam: Elsevier, 1989, 189–210
[2]CSIRO. Unlocking Success Through Change and Innovation: Options to Improve the Profitability and Environmental Performance of the Australian Sugar Industry. Submission to Sugarcane Industry Assessment, 2005. p 4
[3]Garcés F F, Fiallos F F, Silva E, Martinez F, Aime M C, Comstock J C, Glynn N C, Castlebury L A. First report of orange rust of sugarcane caused by Puccinia kuehnii in Ecuador. Plant Dis, 2014, 98: 842
[4]Hoy J W, Hollier C A. Effect of brown rust on yield of sugarcane in Louisiana. Plant Dis, 2009, 93: 1171–1174
[5]Comstook J C, Shine J M, Raid R N. Effect of early rust infection on subsequent sugarcane growth. Sugar Cane, 1992, 4: 7–9
[6]马利. 泰国甘蔗生产及科研现状. 世界农业, 1995, 9: 23
Ma L. The production and research actuality of sugarcane in Thailand. World Agric, 1995, 9: 23 (in Chinese)
[7]阮兴业, 杨雾, 孙楚坚. 云南省发现甘蔗茅柄锈菌. 真菌学报, 1983, 2: 260–261
Ruan X Y, Yan F, Sun C J. Occurrence of Puccinia erianthi on sugarcane in Yunnan province. Acta Mycologica Sin. 1983, 2: 260–261 (in Chinese)
[8]全国甘蔗重要病害研究协作组. 我国大陆植蔗省(区)(部分)甘蔗病害种类调查初报. 甘蔗糖业, 1991, (1): 1–8
The important sugarcane diseases research cooperation group. The preliminary report of sugarcane diseases investigation in the sugarcane planting provinces (partly), Mainland China. Sugarcane Canesugar, 1991, (1): 1–8 (in Chinese with English abstract)
[9]刘晓妹, 刘文波, 施焕焕. 海南儋州甘蔗锈病病原菌的鉴定及其生物学特性的测定. 中国糖料,2008, (2): 30–32
Liu X M, Liu W B, Shi H H. Pathogen identification and biological characteristics of sugarcane rust in Danzhou. Sugar Crops China, 2008, (2): 30–32 (in Chinese with English abstract)
[10]韦金菊, 邓展云, 黄伟华,  潘雪红, 王伯辉, 刘晓静. 广西北海甘蔗锈病生物学特性研究及防治对策. 安徽农业科学, 2010, 38: 14997–14999
Wei J J, Deng Z Y, Huang W H, Pan X H, Wang B H, Liu X J. Control methods and pathogen biological characteristics of sugarcane rust in Beihai. J Anhui Agric Sci, 2010, 38: 14997–14999 (in Chinese with English abstract)
[11]黄应昆, 李文凤. 云南蔗区甘蔗锈病流行原因及防治对策. 植保技术与推广, 1998, 18: 22–23
Huang Y K, Li W F. Epidemic and control strategies of sugarcane rust disease in Yunnan sugarcane field. Plant Prot Technol Extens, 1998, 18: 22–23 (in Chinese)
[12]黄应昆, 李文凤. 现代甘蔗病虫草害原色图谱. 北京: 中国农业出版社, 2011. pp 104–106
Huang Y K , Li W F. Colored Atlas of Main Diseases, Insect Pests and Weeds of Modern Sugarcane. Beijing: China Agriculture Press, 2011. pp 104–106 (in Chinese)
[13]Comstock J C, Wu K K, Schnell R J. Heritability of resistance to sugar cane rust. Sugar Cane (United Kingdom), 1992, 6: 7–10
[14]Hogarth D M, Ryan C C, Taylor P W J. Quantitative inheritance of rust resistance in sugarcane. Field Crops Res, 1993, 34: 187–193
[15]Ramdoyal K, Sullivan S, Chong L C Y L S, Badaloo G H, Saumtally S, Domaingue R. The genetics of rust resistance in sugar cane seedling populations. Theor Appl Genet, 2000, 100: 557–563
[16]Daugrois J H, Grivet L, Roques D. A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar‘R570’. Theor Appl Genet, 1996, 92: 1059–1064
[17]Asnaghi C, Roques D, Ruffel S, Kaye C, Hoarau J Y, Télismart H, Girard J C, Roboin L M, Risterucci A M, Grivet L, D’Hont A. Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers. Theor Appl Genet, 2004, 108: 759–764
[18]Le Cunff L, Garsmeur O, Raboin L M, Pauquet J, Telismart H, Selvi A, Grivet L, Philippe R, Begum D, Deu M. Diploid/polyploid syntenic shuttle mapping and haplotypespecific chromosome walking toward a rust resistance gene (Bru1) in highly polyploid sugarcane (2n–12x * 115). Genetics, 2008, 180: 649–660
[19]Asnaghi C, D’hont A, Glaszmann J, Rott P Resistance of sugarcane cultivar R 570 to Puccinia melanocephala isolates from different geographic locations. Plant Dis, 2001, 85: 282–286
[20]Costet L, Toubi L, Le Cunff L. Haplotype structure around Bru1 reveals a narrow genetic basis for brown rust resistance in modern sugarcane cultivars. Theor Appl Genet, 2012, 125: 825–836
[21]李文凤, 王晓燕, 黄应昆,张荣跃, 单红丽, 尹炯, 罗志明.31份甘蔗野生核心种质资源褐锈病抗性鉴定及Bru1基因的分子检测. 作物学报, 2015, 41: 806?812
[22]Li W F, Wang  X Y, Huang Y K , Zhang R Y, Shan H L, Yin J, and Luo Z M. Identification of resistance to brown rust and molecular detection of bru1 gene in 31 wild core sugarcane germplasms. Acta Agron Sin, 2015, 41: 806?812 (in Chinese with English abstract)
[23]李文凤, 黄应昆. 现代甘蔗病害诊断检测与防控技术. 北京: 中国农业出版社, 2012. p 94
[24]Li W F, Huang Y K. Diagnosis Detection and Control Technique of Modern Sugarcane Diseases. Beijing: China Agriculture Press, 2012. p 94 (in Chinese)
[25]陈辉, 范源洪, 史宪伟, 蔡青, 张明, 张亚平. 甘蔗细茎野生种的的遗传多样性与系统演化研究. 作物学报, 2001, 27: 645–652
[26]Chen H, Fan Y H, Shi X W, Cai Q, Zhang M, Zhang Y P. Research on genetic diversity and systemic evolution in Saccharum spontaneum L. Acta Agron Sin, 2001, 27: 645–652 (in Chinese with English abstract)
[27]范源洪, 陈 辉, 史宪伟, 蔡青, 张明, 张亚平. 甘蔗细茎野生种云南不同生态类型的RAPD分析. 云南植物研究, 2001, 23: 298–308
[28]Fan Y H, Chen H, Shi X W, Cai Q, Zhang M, Zhang Y P. RAPD analysis of Saccharum spontaneum from different ecospecific colonies in Yunnan. Acta Bot Yunnanica, 2001, 23: 298–308 (in Chinese with English abstract)
[29]Glynn N C, Laborde C, Davidson R W, Irey M S, Glaz B, D'Hont A, Comstock J C. Utilization of a major brown rust resistance gene in sugarcane breeding. Mol Breed, 2013, 31: 323–331
[30]Racedo J, Perera M F, Bertani R, Funes C, Gonzalez V, Cuenya M I, D’Hont A, Welin B, Castagnaro A P. Bru1 gene and potential alternative sources of résistance to sugarcane brown rust disease. Euphytica, 2013, 191: 429–436

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