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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (11): 1647-1655.doi: 10.3724/SP.J.1006.2016.01647

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

RNAi-mediated SMV-P3 Silencing Increases Soybean Resistance to Soybean Mosaic Virus

YANG Xiang-Dong,NIU Lu,ZHANG Wei,YANG Jing,DU Qian,XING Guo-Jie,GUO Dong-Quan,LI Qi-Yun,DONG Ying-Shan   

  1. Jilin Academy of Agricultural Sciences, Agricultural Biotechnology Institute / Jilin Provincial Key laboratory of Agricultural Biotechnology, Changchun 130033, China
  • Received:2016-02-16 Revised:2016-06-20 Online:2016-11-12 Published:2016-07-04
  • Contact: Dong Yingshan, E-mail: ysdong@cjaas.com, Tel: +86-431-87063008 E-mail:xdyang020918@126.com
  • Supported by:

    This study was supported by the China National Novel Transgenic Organisms Breeding Project (2016ZX08004-004) and the Science & Technology Development Project of Jilin Province in China(20150204011NY)。

Abstract:

Soybean mosaic virus (SMV) is one of the most important diseases in major soybean production areas and has severe effects on soybean production and seed quality in China. Breeding disease-resistant varieties is the most economical and effective strategy to prevent and control SMV. In this study, RNAi fragments of the gene encoding P3 protein, which is involved in SMV mobility and affecting host range, were introduced into soybean by plant-mediated RNA interference (RNAi) techniques to explore the influence of RNAi-mediated SMV-P3 silencing on soybean SMV resistance. Southern blot analysis revealed that exogenous RNAi fragments were integrated into the soybean genome at low copy numbers (1–4). T1–T3 generation transgenic soybeans were sprayed with herbicide and inserted fragments were examined using PCR. The results indicated that T-DNA insertion fragments could be stably inherited between generations of transgenic soybean. Inoculation of T2 and T3 generation transgenic soybeans with SMV suggested that transgenic soybeans exhibited significantly higher resistance to the prevailing SMV strain, SC-3, in major soybean production areas than the non-transgenic control varieties Williams 82 and SN9. The disease index was reduced by 4.37%-18.51%. Further, the resistance could be stably inherited. In conclusion, RNAi-mediated SMV-P3 silencing can significantly increase the SMV resistance of transgenic soybeans.

Key words: Soybean, Soybean mosaic virus, SMV-P3, RNAi-mediated gene silencing

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