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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (11): 1638-1646.

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analysisof Blast Resistance Genesin Japonica Variety Jijing 809

ZHU Ya-Jun1,**, SUN Qiang2,**, WANG Jin-Ming2, CHEN Kai1, FENG Bo1, FANG Ya-Jie1, LIN Xiu-Yun2,*, and XU Jian-Long1,3,*   

  1. 1 National Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Rice Research Institute, Jilin Academy of agricultural Sciences, Gongzhuling 130000, China; 3 Shenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
  • Received:2016-08-01 Revised:2016-08-02 Online:2016-11-12 Published:2016-08-11
  • Contact: 徐建龙,E-mail:xujlcaas@126.com,Tel:010-82105854;林秀云,E-mail:linxiuyun1965@163.com,Tel:13504462960
  • Supported by:

    ThisstudywassupportedbytheProgramofIntroducingInternationalSuperAgriculturalScienceandTechnology(948Program,2010-G2B-2),ProjectoftransformationofscientificandtechnologicalachievementsinJilinProvince(20130305038NY),theNationalHighTechnologyResearchandDevelopmentProgramofChina(863Program,2014AA10A601),andNaturalScienceFoundationofJilinProvince(20140101014JC).

Abstract:

Blast is one of the most hazardous diseases in rice production. Planting resistance variety is an effective measure to control the disease. In this study, a backcross population derived from two parents, Jijing 88 and 93072, was selected to identify blast resistance genes using bulked extremes and recessive class under artificial inoculation and further to deduce the composition of resistance genes in Jijing 809 developed from Jijing 88 and 93072. Four major resistance genes including Pi-2(t), Pi-7-1(t), Pi-7-2(t), and Pi-11(t) were responsible for segregation of resistance to the strong virulent strain GD9-1, and only one resistance gene Pi-2(t) for segregation of resistance to the weak virulent strain GD19-1. Among them, Pi-2(t) was effective to the two strains. The favorable alleles at all loci were from 93072 except for the allele at Pi-2(t) from Jijing 88. By comparative mapping, Pi-2(t) was deduced to be allelic to Pi-b, and Pi-11(t) to Pi-47(t) or Pik, whereas Pi-7-1(t) and Pi-7-2(t) were the two newly identified resistant genes, having linkages with RM21260 and RM8037 at 0.11 and 6.97 cM, respectively. SSR markers tight by linked with the four resistance genes mentioned above and a set of 55K gene chip developed from re-sequenced data of 3K rice germplasm were used to compare and identify genotypes of Jijing 809 at the above resistant loci with the two parents. It was indicated that two pairs of resistant genes, Pi-2(t) and Pi-11(t), in Jijing 809 were from the recurrent parent Jijing 88 and the donor parent 93072, respectively, which reasonably explained that Jijing 809 had much better blast resistance than Jijing 88. It was discussed how to pyramid different known major resistant genes, especially to make full use of the ‘defeated’ resistance gene in the original resistant variety to improve blast resistance of rice.

Key words: Rice, Blast, Resistance gene, Bulked extremes and recessive class, Pyramiding of resistance gene

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