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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (04): 473-482.doi: 0.3724/SP.J.1006.2017.00473

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Identification and Gene Mapping of Starch Accumulation and Early Senescence Leaf Mutant esl9 in Rice

XIAO Yan-Hua**, CHEN Xin-Long**, DU Dan, XING Ya-Di, ZHANG Tian-Quan, ZHU Mao-Di,LIU Ming-Ming,ZHU Xiao-Yan, SANG Xian-Chun,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400716, China
  • Received:2016-09-11 Revised:2017-01-21 Online:2017-04-12 Published:2017-02-10
  • Contact: HE Zhonghua, E-mail: hegh@swu.edu.cn E-mail:xyhua0625@163.com
  • Supported by:

    This study was supportedbythe Special Industry Project ofMinistry of Agriculture (201303129) and the Capacity PromotionProject of Key Laboratories in Chongqing (cstc2014pt-sy80001).

Abstract:

A new leaf senescence mutant esl9(early senescence leaf9) was discovered from the progeny of indica maintainer line 1B mutatedby ethylmethanesulfonate(EMS). Compared with the wild-type, the leaf of esl9 was pale green at seedling stage; chlorosis occurredatleaf tipand gradually extendedto the middle-upper parts of leaf at tillering stage. However, the leaf base remained green untilmaturity. In theesl9, the photosynthetic pigment contentsdeclined,and the contentsof reactive oxygen species (ROS), such as, O2, ·OH and H2O2, heightened compared with those in the wild-type. At the same time, activities of protective enzymes, SOD and CAT, both reduced in esl9. The results of iodine-iodide kalium dyeing and starch content determinationshowed that more starch granules accumulated in the esl9 leaf. Quantitative RT-PCR results indicatedthat genes responsible for starch synthesis were up-regulated and genes participated in the triose phosphatedistribution path were down-regulated. We made an inferencethatgene mutation changed the distribution of triose phosphate, resulting in starch granules accumulating in the leaf, chloroplast structure being destroyed and photosynthetic systembeing blocked, thus increasingthe contents of ROS,eventually causing leaf senescence.Genetic analysis demonstrated that the phenotype of esl9 was controlled by a dominant nuclear gene. The target geneESL9 was mapped between SSR markers S11-110 and S11-87 with a physical distance of 304.9 kbon chromosome 11. These results will lay a foundation for cloning and functionallyanalysing ESL9.

Key words: Rice(Oryza sativa L.), Early senescence, Starch accumulation, Genetic analysis, Gene mapping

[1] Sedigheh H G, Mortazavian M, Norouzian D, Atyabi M, Akbarzadeh A, Hasanpoor K, Ghorbani M. Oxidative stress and leaf senescence. BMC Res Notes, 2011, 4:477–485
[2] Lee R H, Lin M C, Grace Chen S C. A novel alkaline a-galactosidase gene is involved in rice leaf senescence.Plant Mol Biol, 2004, 55: 281–295
[3] Kong Z S, Li M N, Yang W Q, Xu W Y, Xue Y B. A novel nuclear-localized CCCH-Type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice. Plant Physiol, 2006, 141: 1376–1388
[4] Wang J, Wu S J , Zhou Y, Zhou L H, Xu J F, Hu J, Fang Y X, Gu M H, Liang G H. Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.). Chin Sci Bull, 2006, 51: 2986–2992
[5] Zhu L, Liu W Z, Wu C, Luan W J, Fu Y P, Hu G C, Si H M, Sun Z X. Identification and fine mapping of a gene related to pale green leaf phenotype near the centromere region in rice (Oryza sativa). Rice Sci, 2007, 14: 172–180
[6] Fang L K, Li Y F, Gong X P, Sang X C, Ling Y H, Wang X W, Cong Y F, He G H. Genetic analysis and gene mapping of a dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.). Chin Sci Bull, 2010, 55: 2517–2521
[7] 徐芳芳, 桑贤春, 任德勇, 唐彦强, 胡宏伟, 杨正林, 赵芳明, 何光华. 水稻早衰突变体esl2的遗传分析及基因定位.作物学报, 2012, 38: 1347–1353
Xu F F, Sang X C, Ren D Y, Tang Y Q, Hu H W, Yang Z L, Zhao F M, He G H. Genetic analysis and gene mapping of early senescence leaf mutant esl2 in rice. Acta Agron Sin, 2012, 38: 1347–1353 (in Chinese with English abstract)
[8] 苗润隆, 蒋钰东, 廖红香, 徐芳芳, 何光华, 杨正林, 赵芳明, 桑贤春.水稻早衰突变体esl3的鉴定与基因定位. 作物学报, 2013, 39: 862−867
Miao R L, Jiang Y D, Liao H X, Xu F F, He G H, Yang Z L, Zhao F M, Sang X C.Identification and gene mapping of rice early senescent leaf (esl3) mutant. Acta Agron Sin, 2013, 39: 862−867 (in Chinese with English abstract)
[9] Guo S, Zhang T Q, Xing Y D, Zhu X Y,Sang X C, Ling Y H, Wang N, He G H. Identification and gene mapping of an earlysenescence leaf 4 mutant of rice. Crop Sci, 2014, 54: 2713–2723
[10] 桑贤春, 徐芳芳, 朱小燕, 邢亚迪, 何沛龙, 张长伟, 杨正林, 何光华. 水稻早衰突变体esl5的鉴定及其基因精细定位. 作物学报, 2014, 40: 1182–1189
Sang X C, Xu F F, Zhu X Y, Xing YD, He P L, Zhang C W, Yang Z L, He G H.Identification and gene fine mapping of early senescent leaf mutant esl5 inOryza sativa. Acta Agron Sin, 2014, 40: 1182–1189 (in Chinese with English abstract)
[11] 杨波, 夏敏, 张孝波, 王晓雯, 朱小燕, 何沛龙, 何光华, 桑贤春. 水稻早衰突变体esl6的鉴定与基因定位. 作物学报, 2016, 42: 976–983
Yang B, Xia M, Zhang X B, Wang X W, Zhu X Y, He G H, Sang X C. Identification and gene mapping of an early senescent leaf mutant esl6 in Oryza sativa. Acta Agron Sin, 2016, 42: 976–983 (in Chinese with English abstract)
[12] 杜青, 方立魁, 桑贤春, 凌英华, 李云峰, 杨正林, 何光华, 赵芳明. 水稻叶尖早衰突变体lad的形态、生理分析与基因定位. 作物学报, 2012, 38: 168–173
Du Q, Fang L K, Sang X C, Ling Y H, Li Y F, Yang Z L, He G H, Zhao F M. Analysis of phenotype and physiology of leaf apex dead mutant(lad)in rice and mapping of mutant gene. Acta Agron Sin, 2012, 38: 168–173 (in Chinese with English abstract)
[13] Shi B, Ni L, Zhang A Y, Cao J M, Zhang H, Qin T T, Tan M P, Zhang J H, Jiang M Y. OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidantdefense in leaves of rice. Mol Plant, 2012, 5: 1359–1374
[14] Jiao B B, Wang J J, Zhu X D, Zeng L J, Li Q, He Z H. A novel protein RLS1 with NBARM domains is involved in chloroplast degradation during leaf senescence in rice. Mol Plant, 2012, 5: 205–217
[15] Hu B, Zhu C G, Li F, Tang J Y, Wang Y Q, Lin A H, Liu L C, Che R H, Chu C C. Leaf tip necrosis1 plays a pivotal role in the regulation of multiple phosphate starvation responses in rice. Plant Physiol, 2011, 156: 1101–1115
[16]Lin A H, Wang Y Q, Tang J Y, Xue P, Li C L, Liu L C, Hu B, Yang F Q, Loake G J, Chu C C. Nitric oxide and protein S-nitrosylation are integral to hydrogen peroxide-induced leaf cell death in rice. Plant Physiol, 2012, 158: 451–464
[17] Qiao Y L, Jiang W Z, Lee J H, Park B S, Choi M S, Piao R H, Woo M O, Roh J H, Han L Z, Paek N C, Seo H S, Koh H J. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit l1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol, 2010, 185: 258–274
[18] Smith A M, Zeeman S C, Thorneycroft D, Smith S M. Starch mobilization in leaves. J Exp Bot, 2003, 54: 577–583
[19] Goldschmidt E E, Huber S C. Regulation of photosynthesis by end-product accumulation inleaves of plants storing starch, sucrose, and hexose sugars. Plant Physiol, 1992, 99: 1443–1448
[20] Hirose T, Aoki N, Harada Y, Okamura M, Hashida Y, Ohsugi R, Miyao A, Hirochika H, Terao T. Disruption of a rice gene for α-glucan water dikinase, OsGWD1, leads to hyperaccumulation of starch in leaves but exhibits limited effects on growth. Front Plant Sci, 2013, 4: 147–156
[21] Yun M S, Umemoto T, Kawagoe Y. Rice debranching enzyme isoamylase3facilitates starch metabolism and affects plastid morphogenesis. Plant Cell Physiol, 2011, 52: 1068–1082
[22] Wellburn A R. The spectral determination of chlorophylls a and b as well as total carotenoids using varioussolvents with spectrophotometers of different resolution. Plant Physiol, 1994, 144: 307–313
[23] Arnon D L. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol, 1949, 24:1–15
[24] 何瑞锋, 丁毅, 余金洪, 祖明生. 水稻温敏叶绿素突变体叶片超微结构的研究. 武汉植物学研究, 2001, 19(1): 1–5
He RF, Ding Y, Yu JH, Zu MS. Study on leaf ultrastructure of the thermo-sensitive chlorophyll deficient mutant in rice. J Wuhan Bot Res, 2001, 19(1): 1–5 (in Chinese with English abstract)
[25]王瑞庆, 马书尚, 张继澍.淀粉-碘染色法确定苹果成熟度.西北农林科技大学学报(自然科学版), 2010, 38(9): 81–94
Wang R Q, Ma S S, Zhang J S. Starch iodine test for determining maturation of apple fruit. JNorthwest A&F Univ(Nat Sci Edn), 2010, 38(9): 81–94 (in Chinese with English abstract)
[26] Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep, 1997, 15: 8–15
[27] Michelmore R W, Paran I, Kessel R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[28] Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Genet Genomics, 1996, 252: 597–607
[29] Xu Q Z, Huang B R. Antioxidant metabolism associated with summer leaf senescence and turf quality decline for creeping bentgrass. Crop Sci, 2004, 44: 553–560
[30] Lim P O, Kim H J, Nam H G. Leaf senescence. Annu Rev Plant Biol, 2007, 58: 115–136
[31] Lloyd J R, Kossmann J, Ritte G. Leaf starch degradation comes out of the shadows. Trends Plant Sci, 2005, 10: 1360–1385
[32] Schaffer A A, Liu K C, Goldschmidt E E, Boyer C D, Goren R. Citrus leaf chlorosis induced by sink removal: starch, nitrogen, and chloroplast ultrastructure.JPlant Physiol, 1986, 124: 111–121
[33] Edreva A. Generation and scavenging of reactive oxygen species in chloroplasts: a submolecular approach. Agric Ecosyst Environ, 2005, 106: 119–133
[34] Alscher R G, Erturk N, Heath L S. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot, 2002, 53: 1331–1341
[35] Foyera C H, Noctor G. Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiol Plant, 2003, 119: 355–364
[36] Chow F, Pedersén M, Oliveira M C. Modulation of nitrate reductase activity by photosynthetic electron transport chain and nitric oxide balance in the red macroalga Gracilaria chilensis (Gracilariales, Rhodophyta). J Appl Phycol, 2013, 25: 1847–1853
[37] Ooijen G V, Mayr G, Kasiem M M A, Albrecht M, Cornelissen B J C, Takken FL W. Structure-function analysis of theNB-ARC domain of plant disease resistance proteins. J Exp Bot, 2008, 59: 1383–1397

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