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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (12): 1991-1996.doi: 10.3724/SP.J.1006.2020.03025

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic analysis and characterization of male sterile mutant mi-ms-3 in maize

TIAN Shi-Ke(), QIN Xin-Er, ZHANG Wen-Liang, DONG Xue, DAI Ming-Qiu, YUE Bing*()   

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2020-05-07 Accepted:2020-08-19 Online:2020-08-31 Published:2020-08-31
  • Contact: YUE Bing E-mail:tianshike1996@163.com;yuebing@mail.hzau.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2016YFD0100804)

Abstract:

Maize is one of the best crops in the utilization of heterosis. Male sterile lines are important germplasms for the hybrids production. A male sterile mutant named mi-ms-3 was obtained by screening in a mutator insertion library. The number of male anthers in tassel decreased and not exserted. There were few anthers with only two pollen sacs in the mutant tassels, and some of the anthers were degenerated to membranous and formed filaments at their ends. Although pollens in the anthers could be stained by I2-KI, pollen shedding was abnormal and the number of pollen grains decreased. The number of silks in the ear of the mutant increased, and there was a sterile grain on both sides of the maturated kernel. Fertility of F1 plants, which were obtained by hybridization between mi-ms-3 and maize inbred Mo17, was normal. Genetic analysis of F2 population showed that the mutant phenotype was controlled by a recessive gene. The candidate gene was preliminarily mapped on the long arm of chromosome 3 by BSA and it was located between a SSR marker and an Indel marker with a distance of 1.5 cM. There are 21 candidate genes in this region. It was finally found that the insertion mutation of Mu transposon occurred at 30 bp upstream of the coding region of zm00001d042618 (zmm16) by transponson tagging and sequencing analysis. The results showed that mi-ms-3 was a new allele of sts1, which caused by a single base mutation in the coding region. RT-PCR analysis indicated that the expression of zmm16 in the mutant was decreased. The identification of the new allelic mutant of sts1 in this study would provide new materials for the study of flower development and hybrid seed production.

Key words: maize, male sterile, gene mapping, genetic analysis

Table 1

Primers for genetic mapping and gene expression analysis"

引物名称
Primer name
正向引物
Forward sequence (5′-3′)
反向引物
Reverse sequence (5′-3′)
染色体位置
Chromosome position
umc1973 CAGGCAGAAAAGGAACGGAAC GTGCGAGAGAAGATGGATGATTG 3.05
umc1027 AACTCTGTCTCCGTCACCGTGT GACCTCATCTCGGTGGAAATTG 3.06
S-1 CCGTCATCGACATTTTACTCAG CACAAGAGTTGGTTCGCTG 3.06
S-2 CCTTGTACCCGCTCATCCAT CTCCTACCGACCTGCCTG 3.06
S-3 GTGTTACAAGAACTCAGCGT AGCCTTCGTTCAGTTTCGGT 3.06
S-4 TGACTGGCTTTCGTCCTACT CACTCTCCAGCTGCTTCTCT 3.06
S-5 ACAATTAGCTTAAGAACGC AGCGGTGTTCATGGTACTCA 3.06
S-6 TGGCTACTAGCTAGCTGTGCT CTAAACCTTCACGCACAGCC 3.06
S-7 CCAACTGAGCCGAATCGTTC ATCATCCCATATCCCACGCC 3.06
S-8 GTGGCCCATATGGTCTCAAC CGGCCAGTCGATTAAAGCTC 3.06
S-9 CGACGAAGAACTGACTTGACC TTGCTTGATCATTCCTCCGC 3.06
S-10 GTTTGGGCCGCATTCGAGAGA TATTCTTGCCTCTCCCACCG 3.06
TIR-1 CGGCCTCCATTTCGTCGAATCCCTT
42618 TGCCGGTACTGTGACTGTTC CCTGAGCTCGATCTGCATGG 3.06
42618-2 TCTTTACTCCTCCCCTCCCA CTTCTTGAGGATCCCGTTGC 3.06
q42618 GCCTTAGTGCAGAGATTGACC GCCTTTCCCAGTGCTCCA 3.06

Fig. 1

Phenotypic of the mi-ms-3 and wild type (WT) A: the tassel of WT(wild type) (left) and mi-ms-3 (right), mi-ms-3 anther not exserted; B1-B2: transverse sections of the entire anther of WT and mi-ms-3 in the late microspore developmental stage, mi-ms-3 anthers with only two pollen sacs; C1-C4: the spikelet of mi-ms-3 contain 0-3 anthers respectively; D1-D2: pollen grains of WT and mi-ms-3; E1: the ear of mi-ms-3 (left) and WT (right), the number of silks in the ear of the mi-ms-3 increased; E2: the kernel of mi-ms-3 (left) and WT (right), mi-ms-3 each seed corresponds to multiple silks."

Table 2

Genotype and phenotype of the important recombinant individuals"

umc1973 S-1 S-2 S-3 S-4 S-5 S-6 S-7 S-8 S-9 S-10 umc1027 表型Phenotype
2-2 H H H H A A A A A A A A 突变体Mutant
11-5 H H H H H A A A A A A A 突变体Mutant
9-3 H H H H H H H A A A A A 突变体Mutant
14-8 A A A A A A A A A A A H 突变体Mutant
5-4 H H H H H H H H H H H A 野生型Wild type
20-9 A H H H H H H H H H H H 野生型Wild type

Fig. 2

Identification of insertion mutant of mu transposon 1-2: heterozygous plants; 3-5: homozygous wild type plants; 6: marker; 7-14: homozygous mutant plants; 15: B73; 16: Mo17."

Fig. 3

Sequence analysis of zmm16 Mu13 transposon occurred at 30 bp upstream of ATG of zmm16."

Fig. 4

Prediction and analysis of promoter elements of zmm16"

Fig. 5

Expression level of zmm16 The expression of zmm16 in mi-ms-3 decreased compared with wild type."

[1] 马冲, 张春庆, 陈举林, 侯玮, 王国胜 . 玉米胞质雄性不育系研究进展. 中国农学通报, 2005,21(1):163-164.
Ma C, Zhang C Q, Chen J L, Hou W, Wang G S . Advance on study of male sterility in maize. Chin Agric Sci Bull, 2005,21(1):163-164 (in Chinese with English abstract).
[2] Tanaka W, Pautler M, Jackson D, Hirano H Y . Grass meristems II: inflorescence architecture, flower development and meristem fate. Plant Cell Physiol, 2013,54:313-324.
doi: 10.1093/pcp/pct016
[3] Li Q L, Liu B S . Genetic regulation of maize flower development and sex determination. Planta, 2017,245:1-14.
doi: 10.1007/s00425-016-2607-2 pmid: 27770199
[4] Ambrose B A, Lerner D R, Ciceri P, Padilla C M, Yanofsky M F, Schmidt R J . Molecular and genetic analyses of the silky1 gene reveal conservation in floral organ specification between eudicots and monocots. Mol Cell, 2000,5:569-579.
doi: 10.1016/s1097-2765(00)80450-5 pmid: 10882141
[5] Bartlett M E, Williams S K, Taylor Z, DeBlasio S, Goldshmidt A, Hall D H, Schmidt R J, Jackson D P, Whipple C J . The maize PI/GLO ortholog zmm16/sterile tassel silky ear1 interacts with the zygomorphy and sex determination pathways in flower development. Plant Cell, 2015,27:3081-3098.
doi: 10.1105/tpc.15.00679 pmid: 26518212
[6] 王关林, 方宏筠 . 植物基因工程. 北京: 科学出版社, 2002. pp 742-744.
Wang G L, Fang H J . Plant Genetic Engineering. Beijing: Science Press, 2002. pp 742-744(in Chinese).
[7] Settles A M, Latshaw S, McCarty D R . Molecular analysis of high-copy insertion sites in maize. Nucl Acids Res, 2004,32:e54.
doi: 10.1093/nar/gnh052 pmid: 15060129
[8] Chen C, Chen H, Yi Z, He Y, Hannah T R, Frank M H, Xia R . Tbtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant, 2020,13:1194-1202.
doi: 10.1016/j.molp.2020.06.009 pmid: 32585190
[9] Acosta I F, Helene L, Romero S P, Eric S, Mats H, Mottinger J P, Moreno M A, Dellaporta S L . Tasselseed1 is a lipoxygenase affecting jasmonic acid signaling in sex determination of maize. Science, 2009,323:262-265.
doi: 10.1126/science.1164645 pmid: 19131630
[10] Chuck G . Molecular mechanisms of sex determination in monoecious and dioecious plants. Adv Bot Res, 2010,54:53-83.
[11] Zhao Y, Zhang Y Z, Wang L J, Wang X R, Xu W, Gao X Y, Liu B S . Mapping and functional analysis of a maize silkless mutant sk-A7110. Front Plant Sci, 2018,9:1127.
doi: 10.3389/fpls.2018.01127 pmid: 30116254
[12] Sun J, Yang L, Wang J, Liu H L, Zheng H L, Xie D W, Zhang M H, Feng M F, Jia Y, Zhao H W, Zou D T . Identification of a cold-tolerant locus in rice ( Oryza sativa L.) using bulked segregant analysis with a next-generation sequencing strategy. Rice, 2018,11:24.
doi: 10.1186/s12284-018-0218-1 pmid: 29671148
[13] Bennetzen J L, Springer P S, Cresse A D, Hendrickx M . Specificity and regulation of the mutator transposable element system in maize. Crit Rev Plant Sci, 1993,12:57-95.
doi: 10.1080/07352689309382356
[14] Wang D X, Skibbe D S, Walbot V . Maize male sterile 8 (Ms8), a putative b-1,3-galactosyltransferase, modulates cell division, expansion, and differentiation during early maize anther development. Plant Reprod, 2013,26:329-338.
doi: 10.1007/s00497-013-0230-y
[15] Cui X Q, Hsia A P, Liu F, Ashlock D A, Wise R P, Schnable P S . Alternative transcription initiation sites and polyadenylation sites are recruited during Mu suppression at the rf2a locus of maize. Genetics, 2003,163:685-698.
pmid: 12618406
[16] 林晓怡, 杨典洱, 林建兴 . 带遗传标记的玉米基因雄性不育的发现及遗传和利用研究. 作物学报, 2000,26:129-133.
Lin X Y, Yang D E, Lin J X . The discovery, inheritance and utilization of genic male sterility with genetic marker in maize. Acta Agron Sin, 2000,26:129-133 (in Chinese with English abstract).
[17] 王颖, 麦维军, 梁成邺, 张明永 . 高等植物启动子的研究进展. 西北植物学报, 2003,23:2040-2048.
Wang Y, Mai W J, Liang C Y, Zhang M Y . Advances on studies of plant promoters. Acta Bot Boreal-Occident Sin, 2003,23:2040-2048 (in Chinese with English abstract).
[18] Becker A, Theissen G . The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Mol Phylogenet Evol, 2003,29:464-489.
doi: 10.1016/s1055-7903(03)00207-0 pmid: 14615187
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