甘蓝型油菜早熟性状QTL定位及候选基因筛选

doi:10.3724/SP.J.1006.2021.04145

Abstract

Abstract:

Currently, the research on earliness traits of rapeseed mainly focused on flowering time. Although there was a significant positive correlation between the flowering period and the growth period, it was not completely consistent. There are few reports on the genetic studies and QTL mapping of traits related to rapeseed growth and development after flowering. We carried out phenotypic survey (flowering time, flowering period duration, silique period duration etc.) and QTL mapping with the DH population constructed by ‘Huaqianzao’ and ‘Global’ as material. A total of 30 QTL loci for earliness traits were detected. Among them, 12, 5, 4 and 9 QTL loci were detected in the flowering time, flowering period duration, silique period duration and full growth period, respectively, explaining 5.8%-22.4% phenotypic variance. The 2, 4, and 1 QTL confidence intervals of full growth period were found overlap in part or in whole with flowering time, flowering period duration and silique period duration respectively. The 29 candidate genes were screened, which affected the earliness traits by regulating flowering or silique development in rapeseed growth and development processes. Therefore, in the study of earliness traits, we could consider flowering time and silique development process at the same time, which not only helps to advance the maturity period, but also reduces the adverse effects of early flowering.

Key words: Brassica napus L., earliness traits, QTL mapping, candidate genes

LI Shu-Yu, HUANG Yang, XIONG Jie, DING Ge, CHEN Lun-Lin, SONG Lai-Qiang. QTL mapping and candidate genes screening of earliness traits in Brassica napus L.[J].Acta Agronomica Sinica, 2021, 47(4): 626-637.

Figures/Tables 7

Table 1

Fig. 1

Table 2

Table 3

Fig. 2

Table 4

Detected QTLs of earliness related traits in different environments"

位点 Locus	性状 Trait	染色体 Chr.	位置 Pos (cM)	LOD值 LOD value	贡献率 R² (%)	加性效应Additive effect	置信区间Confidence interval	环境 Environment
qFT.A02-1	FT	A02	18.23	5.37	10.6	2.15	16.52-18.52	2018西宁 2018 Xining
qFT.A02-2	FT	A02	60.99	3.99	9.1	-1.82	58.14-61.84	2018西宁 2018 Xining
qFT.A06-1	FT	A06	95.90	6.73	10.9	2.39	95.61-96.75	2018南昌 2018 Nanchang
qFT.A09-1	FT	A09	84.60	5.22	11.8	5.56	84.03-87.84	2016韶关 2016 Shaoguan
qFT.A09-2	FT	A09	123.12	4.04	8.8	3.92	121.65-126.32	2016南昌 2016 Nanchang
qFT.C04-1	FT	C04	99.26	4.32	9.5	5.04	96.73-99.26	2016韶关 2016 Shaoguan
qFT.C06-1	FT	C06	145.13	5.90	9.6	2.29	144.13-149.13	2018南昌 2018 Nanchang
qFT.C07-1	FT	C07	81.72	3.76	5.8	1.69	78.76-84.10	2018南昌 2018 Nanchang
qFT.C08-1	FT	C08	15.37	7.47	12.4	2.05	10.88-15.37	2018西宁 2018 Xining
qFT.C08-2	FT	C08	40.61	6.60	11.1	2.01	34.77-43.49	2018西宁 2018 Xining
	FT	C08	43.49	4.65	9.0	4.00	42.63-47.70	2016南昌 2016 Nanchang
	FT	C08	45.80	12.87	22.4	3.71	45.80-45.80	2018南昌 2018 Nanchang
qFT.C08-3	FT	C08	52.89	6.54	12.5	6.50	52.04-53.75	2016韶关 2016 Shaoguan
qFT.C08-4	FT	C08	104.06	4.57	7.2	2.08	103.27-104.94	2018南昌 2018 Nanchang
qFPD.A02-1	FPD	A02	19.09	4.22	7.3	-1.40	19.09-21.67	2018南昌 2018 Nanchang
qFPD.A06-1	FPD	A06	95.61	6.14	11.0	-1.54	95.33-97.32	2018南昌 2018 Nanchang
qFPD.A09-1	FPD	A09	84.60	4.28	8.9	-2.21	84.03-87.25	2016韶关 2016 Shaoguan
qFPD.C04-1	FPD	C04	123.90	3.79	8.4	-2.42	118.86-127.70	2016南昌 2016 Nanchang
qFPD.C08-1	FPD	C08	45.80	8.24	15.2	-1.95	44.36-46.70	2018南昌 2018 Nanchang
qSPD.A09-1	SPD	A09	91.99	4.59	9.1	-1.19	89.73-92.87	2018西宁 2018 Xining
qSPD.C04-1	SPD	C04	64.02	5.07	10.3	-1.30	62.14-64.30	2018西宁 2018 Xining
qSPD.C08-1	SPD	C08	17.20	4.77	10.2	1.71	13.88-21.20	2016韶关 2016 Shaoguan
qSPD.C08-2	SPD	C08	32.35	5.86	10.1	-1.27	32.35-33.56	2018西宁 2018 Xining
qFGP.A02-1	FGP	A02	50.67	5.50	10.7	-5.35	50.10-51.53	2016韶关 2016 Shaoguan
qFGP.A02-2	FGP	A02	61.56	5.72	11.9	-1.53	58.71-62.13	2018西宁 2018 Xining
qFGP.A09-1	FGP	A09	84.60	4.48	8.8	4.21	83.74-87.25	2016韶关 2016 Shaoguan
qFGP.C06-1	FGP	C06	111.50	7.34	12.0	1.34	111.16-119.50	2018南昌 2018 Nanchang
qFGP.C07-1	FGP	C07	79.33	6.53	10.0	1.16	78.14-80.33	2018南昌 2018 Nanchang
qFGP.C08-1	FGP	C08	5.40	7.56	11.8	1.41	4.54-9.88	2018南昌 2018 Nanchang
qFGP.C08-2	FGP	C08	23.27	6.16	12.5	2.15	21.20-27.89	2016南昌 2016 Nanchang
qFGP.C08-3	FGP	C08	42.05	3.99	7.2	1.21	40.16-44.65	2018西宁 2018 Xining
	FGP	C08	44.07	5.42	10.7	4.84	43.49-44.65	2016韶关 2016 Shaoguan
qFGP.C08-4	FGP	C08	86.77	5.97	9.2	1.21	86.77-87.36	2018南昌 2018 Nanchang

Table 4

Table 5

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环境 Environment	研究材料 Material		开花期 Flowering time	花期持续时间 Flowering period duration	角果期持续时间 Silique period duration	全生育期 Full growth period
2016南昌 2016 Nanchang	亲本 Parents	花前早 Huaqianzao	76.0 A	64 A	32.5 A	172.5 A
	亲本 Parents	Global	138.5 B	36.5 B	30.0 A	205.0 B
	DH群体 DH population	最大值 Max.	141.0	77.0	36.0	205.0
		最小值 Min.	76.0	26.0	22.0	172.5
		均值 Mean	118.5	43.5	29.0	191.0
2016韶关 2016 Shaoguan	亲本 Parents	花前早 Huaqianzao	64.5 A	40.5 A	27.5 A	132.5 A
	亲本 Parents	Global	123.5 B	22.5 B	37.5 B	183.5 B
	DH群体 DH population	最大值 Max.	131.0	58.5	44.5	185.0
		最小值 Min.	58.0	17.0	11.0	124.0
		均值 Mean	97.5	34.2	30.2	161.8
2018南昌 2018 Nanchang	亲本 Parents	花前早 Huaqianzao	108.0 A	46.0 A	29.0 A	183.0 A
	亲本 Parents	Global	149.0 B	22 B	30.0 A	201.0 B
	DH群体 DH population	最大值 Max.	149.0	46.0	34.0	203.0
		最小值 Min.	108.0	17.0	26.0	181.0
		均值 Mean	138.1	26.3	30.1	194.5
2018西宁 2018 Xining	亲本 Parents	花前早 Huaqianzao	56.0 A	31.5 A	31.5 A	119.0 A
	亲本 Parents	Global	72.5 B	41.0 B	16.0 B	129.5 B
	DH群体 DH population	最大值 Max.	98.0	42.0	31.5	135.5
		最小值 Min.	56.0	18.0	9.5	116.5
		均值 Mean	72.5	30.1	20.5	123.9

	开花期 Flowering time	花期持续时间 Flowering period duration	角果期持续时间 Silique period duration	全生育期 Full growth period
开花期 Flowering time	1	-0.880^**	0.041	0.926^**
花期持续时间 Flowering period duration		1	-0.120	-0.701^**
角果期持续时间 Silique period duration			1	0.158^*
全生育期 Full growth period				1

连锁群 Chr.	连锁群总长度 Length (cM)	标记数 No. of markers	相邻标记间的最大间隔 Max. interval (cM)	Bin数目 No. bins	相邻bin间平均距离 Bin interval (cM)
A01	110.96	48,693	4.72	183	0.61
A02	117.38	44,545	5.95	201	0.59
A03	197.46	65,502	10.52	280	0.71
A04	119.03	43,644	6.56	190	0.63
A05	101.61	55,238	3.39	187	0.55
A06	107.28	43,735	4.37	197	0.55
A07	118.95	40,195	5.75	212	0.56
A08	62.56	38,113	1.50	151	0.42
A09	139.70	55,599	8.99	226	0.62
A10	95.92	44,019	3.39	185	0.52
C01	126.88	39,781	5.48	197	0.65
C02	148.14	83,307	8.73	201	0.74
C03	206.71	85,609	7.96	320	0.65
C04	195.49	37,202	9.81	248	0.79
C05	193.22	22,608	6.68	217	0.90
C06	161.24	27,401	17.33	165	0.98
C07	157.87	31,921	5.80	211	0.75
C08	133.07	36,408	5.50	194	0.69
C09	107.28	31,475	6.72	140	0.77
平均Average	136.88	46,052	6.80	206	0.67
合计Total	2600.73	874,995	—	3905	—

QTL mapping and candidate genes screening of earliness traits in Brassica napus L.

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