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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (6): 1109-1113.doi: 10.3724/SP.J.1006.2021.04147

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization of aphid-resistance of a hairy wild Brassica oleracea taxa, B. incana

ZUO Xiang-Jun1,2(), FANG Peng-Peng1,2, LI Jia-Na1,2, QIAN Wei1,2, MEI Jia-Qin1,2,*()   

  1. 1College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
    2Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
  • Received:2020-07-05 Accepted:2020-12-01 Online:2021-06-12 Published:2021-01-10
  • Contact: MEI Jia-Qin E-mail:fanta152521@163.com;jiaqinmay@163.com
  • Supported by:
    The National Key Research and Development Program of China(2018YFD0100500)

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Abstract:

Brassica oleracea contains important vegetables varieties in our daily life, however, the production of Brassica vegetables always suffers from aphids. Identifying resistant resource and understanding corresponding resistance mechanism are of great importance for resistance breeding of crops. In our previous study, a wild relative of B. incana with dense trichomes on leaves and stem was obtained. In the present study, to investigate the resistance of B. incana to aphids and to understand the role of trichomes in resistance, the two parental accessions and their F2:3 lines were inoculated with aphids in lab condition. The results showed that the egg production and lifespan of aphids on hairless leaves (36.7 eggs and 22.2 days) were significantly higher than corresponding values on hairy leaves (8.1 eggs and 13.1 days). To investigate whether a chemical role contributed to the resistance, the contact toxicity, gastric toxicity and anti-feeding ability of the leaf extracts were checked between hairy and glabrous F2:3 lines. None of the extract was found to be with significant contact toxicity and gastric toxicity to aphids, whereas the number of aphids on leaves treated with extract from hairy B. oleracea was significantly lower than that treated by extract of glabrous leaves. This study revealed that the resistance of B. incana to aphids might attribute to both the physical inhibition from trichomes and the avoidance role of its chemical compounds.

Key words: Brassica oleracea, aphid, trichome, resistance identification, resistance characteristics

Fig. 1

Phenotypic performances on hairiness at seedling stage under field condition (the top line) and electron microscope (the bottom line) of two parents"

Fig. 2

Number of eggs and the lifespan of aphids on glabrous and hairy lines ** and *** mean significant differences at the 0.01 and 0.001 probability levels, respectively."

Table 1

Number of died aphids under different treatments"

处理
Treatment		 胃杀试验Gastric toxicity assay 触杀试验Contact toxicity assay
24 h 12 h 24 h 36 h
CK 1.6±1.34 a 1.6±0.97 a 2.2±1.14 a 2.6±1.43 a
T1 1.4±1.14 a 0.8±0.79 a 2.6±1.26 a 3.5±1.58 a
T2 1.8±0.84 a 1.2±1.14 a 2.4±1.17 a 3.2±0.92 a

Fig. 3

Number of attached aphids on leaves treated with different extracts in the anti-feeding experiment * mean significant differences at the 0.05 probability level. Treatments are the same as those given in Table 1."

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