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Acta Agronomica Sinica ›› 2021, Vol. 47 ›› Issue (9): 1834-1840.doi: 10.3724/SP.J.1006.2021.04187

• RESEARCH NOTES • Previous Articles    

Effects of hydrogen peroxide soaking on germination and physiological metabolism of seeds in peanut

HAO Xi(), CUI Ya-Nan, ZHANG Jun, LIU Juan, ZANG Xiu-Wang, GAO Wei, LIU Bing, DONG Wen-Zhao, TANG Feng-Shou*()   

  1. Industrial Crops Research Institute, Henan Academy of Agricultural Sciences / Key Laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture and Rural Affairs / Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou 450002, Henan, China
  • Received:2020-08-12 Accepted:2021-01-21 Online:2021-09-12 Published:2021-02-19
  • Contact: TANG Feng-Shou E-mail:hx1997@163.com;fshtang@126.com
  • Supported by:
    National Key Research and Development Program of China “Physiological Basis and Agronomic Management for High-quality and High-yield of Field Cash Crops”(2018YFD1000900);Henan Agricultural Research System(S2012-5)

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Abstract:

To investigate the effects of hydrogen peroxide soaking on peanut germination and seed physiological metabolism, the seed germination and related physiological indexes after seed soaking were determined using peanut variety Kainong 176 as experimental material. The results showed that the germination vigor of peanut was increased from 0 to 24.45%, and the germination percentage was increased from 39.33% to 90.99% by hydrogen peroxide soaking. Hydrogen peroxide treatment increased the content of gibberellin in peanut seeds and decreased the content of abscisic acid at germination stage. The contents of gibberellin in germinating seeds at 0, 24, and 48 hour(s) were 10.82%, 5.73%, and 18.64% higher than those of control, and the contents of abscisic acid were 44.98%, 36.45%, and 39.70% lower than that of control, respectively. At the same time, hydrogen peroxide treatment enhanced the activities of antioxidant enzyme SOD and CAT, increased the contents of soluble sugar and soluble protein, decreased the content of MDA, promoted the catabolism of macromolecular storage substances such as fat, protein, and starch, and provided more ATP and substrates for protein and nucleic acid synthesis. Studies revealed that hydrogen peroxide could promote peanut seed germination under low-temperature stress by mediating antioxidant enzymes, ABA and GA, and storage matter decomposition.

Key words: peanut, soaking, hydrogen peroxide, germination, physiological metabolism

Table 1

Effects of H2O2 soaking treatments on seed germination in peanut "

处理
Treatment 		萌发率
Germination rate (%) 		发芽势
Germination vigor (%) 		发芽率
Germination percentage (%) 		发芽指数
Germination index
对照CK 72.79±2.21 B 0±0 A 39.33±1.83 A 0±0 A
H2O2浸种H2O2 soaking 97.06±2.94 A 24.45±6.80 B 90.99±2.76 B 2.14±0.49 B

Fig. 1

Effects of H2O2 soaking treatments on the contents of GA and ABA at seed germination stage in peanut CK0′, 0 h, 24 h, and 48 h represent dry seed, seed after soaking (germination 0 hour), germinating seed after 24 hours, germinating seed after 48 hours, respectively. Error bars show the standard deviations of three replicates. Different lowercase letter above the bar means significant difference at the 0.05 probability level."

Fig. 2

Effects of H2O2 soaking treatments on SOD and POD activities at seed germination stage in peanut CK0′, 0 h, 24 h, and 48 h represent dry seed, seed after soaking(germination 0 hour), germinating seed after 24 hours, germinating seed after 48 hours, respectively. Error bars show the standard deviations of three replicates. Different lowercase letter above the bar means significant difference at the 0.05 probability level."

Fig. 3

Effects of H2O2 soaking treatments on CAT activities at seed germination stage in peanut CK0′, 0 h, 24 h, and 48 h represent dry seed, seed after soaking (germination 0 hour), germinating seed after 24 hours, germinating seed after 48 hours, respectively. Error bars show the standard deviations of three replicates. Different lowercase letter above the bar means significant difference at the 0.05 probability level."

Fig. 4

Effects of H2O2 soaking treatments on soluble sugar and soluble protein content at seed germination stage in peanut CK0′, 0 h, 24 h, and 48 h represent dry seed, seed after soaking (germination 0 hour), germinating seed after 24 hours, germinating seed after 48 hours, respectively. Error bars show the standard deviations of three replicates. Different lowercase letter above the bar means significant difference at the 0.05 probability level."

Fig. 5

Effects of H2O2 soaking treatments on MDA content at seed germination stage in peanut CK0′, 0 h, 24 h, and 48 h represent dry seed, seed after soaking (germination 0 hour), germinating seed after 24 hours, germinating seed after 48 hours, respectively. Error bars show the standard deviations of three replicates. Different lowercase letter above the bar means significant difference at the 0.05 probability level."

Fig. 6

Effects of H2O2 soaking treatments on free fatty acids, total amino acids, ATP content, and α-amylase activities at seed germination stage in peanut CK0′, 0 h, 24 h, and 48 h represent dry seed, seed after soaking (germination 0 hour), germinating seed after 24 hours, germinating seed after 48 hours, respectively. Error bars show the standard deviations of three replicates. Different lowercase letter above the bar means significant difference at the 0.05 probability level."

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