甘蓝型油菜白花基因InDel连锁标记开发

doi:10.3724/SP.J.1006.2022.14025

Abstract

Abstract:

InDel is widely distributed across the genome and occurs in a high density and large numbers in a genome. To date, the researches about linkage InDel markers of the white petal gene in B. napus L are very less yet. In this study, we constructed the F₂ mapping population from the cross between DH Y05 (yellow petal) and DH W01 (white petal). Two bulks with 30 yellow petal lines and 30 white petal lines of F₂ population were constructed by mixing an equal amount of DNA. Then two bulks and parents were performed 30× whole-genome re-sequencing. Darmor-bzh as the reference genome was aligned to sequence data from the two bulks and parents. QTL-seq and PoPoolation2 workflow were applied to identify the candidate region of the white petal gene. A major candidate region was identified on chromosome C03 (52-54 Mb) of Darmor-bzh. The insertion-deletion (InDel) sites can be visualized in candidate interval by Integrative Genomics Viewer (IGV). Based on these Indel variations, we used Vector and Blast to design InDel primers. Eight InDel markers closely linked to the white petal gene were screened by Polyacrylamide gel electrophoresis (PAGE). In summary, these results provide a basis for fine mapping white petal gene and InDel molecular marker located on functional genes as well as molecular marker assisted selection breeding.

Key words: Brassica napus L., re-sequencing, white petal genes, InDel markers

WANG Rui, CHEN Xue, GUO Qing-Qing, ZHOU Rong, CHEN Lei, LI Jia-Na. Development of linkage InDel markers of the white petal gene based on whole-genome re-sequencing data in Brassica napus L.[J].Acta Agronomica Sinica, 2022, 48(3): 759-769.

Figures/Tables 5

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引物名称 Primer name	Chr. C03	InDel	正向引物序列 Forward sequence (5°-3°)	反向引物序列 Reverse sequence (5°-3°)
InDel-2	52375493-52375505	D10	GCTAAGCGTGTATCTTATCCT	CGGGAATGGCGATCTAAAAA
InDel-3	52396484	I14	GAAAAACCATTGGAAATGCC	TGGGAAGTTTGTTAAAGGGT
InDel-4	52348627-52348640	D12	GACAAGTCTTGGACAGCTAA	CGAGAGGAATCCAAAGATGT
InDel-5	52377696-52377718	D20	TAGAGAGAGCTTAGACCTCC	CGGCTATGAGTGATGTTCTT
InDel-6	52374279	I12	GGTTCTCTCTCTCTTCAGGTT	TAATACTAATCGAAATTGTTGTCCC
InDel-8	52358540-52358551	D11	ATGCAACATCCAAAACACCT	TTTTGCTCTTTTCGCGTTTG
InDel-9	52358775-52358788	D11	TCTGCGATCACGAATACCT	CAAATCGAAAGGAAAGGAACA
InDel-10	52373701-52373721	D19	ACGTTAAAACAGCTTCACTTTC	TCCGTGTCTGCCAAGTATC

Development of linkage InDel markers of the white petal gene based on whole-genome re-sequencing data in Brassica napus L.

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