马铃薯PAL基因家族的全基因组鉴定及其在非生物胁迫下和块茎花色素苷合成中的表达分析

doi:10.3724/SP.J.1006.2023.24247

Abstract

Abstract:

The phenylalanine ammonia-lyase (PAL) is the rate limiting enzyme and key enzyme in phenylpropane metabolism pathway, which plays an important role in plant growth. In this study, a total of 8 gene family members (StPALs) in potato (Solanum tuberosum L.) were identified by BlastP and Hmmer 3.1 software, and their bioinformation were analyzed by the ExPASy, CELLO, PlantCARE, and other online tool. We analyzed the relative expression pattern of StPAL genes in different tissues of double monoploid (DM) potato, as well as under abiotic stresses by RNA-seq in Potato Genome Sequencing Consortium (PGSC) database. We performed RNA-seq on white, red, and purple tuber skin and flesh of three potato cultivars, and the relative expression levels of StPALs genes in different colors tubers (flesh) of three hybrid progeny potatoes were detected by qPCR. StPAL genes were distributed on chromosomes 3, 5, 9, and 10, and eight StPALs were closely related to tobacco (Nicotiana tabacum) PAL. The cis-acting elements revealed that the promoter regions of StPAL genes contained many elements, including light response, stress response to adversity, hormone response, growth and development, and transcription factor binding elements. The results showed that StPAL2 was specifically expressed in stolons, and StPAL3/5/8 were mainly expressed in tubers and stolons. The relative expression level of StPAL3 gene were down-regulated under mannitol treatment, and the relative expression levels of StPAL3 and StPAL8 genes were up-regulated under heat stress. The above results suggested that StPALs might be involved in the tuber growth and abiotic stress response. By transcriptomic and qPCR analysis, the relative expression levels of 5 StPAL genes (StPAL3/4/5/6/8) were up-regulated in the flesh of color potato, suggesting that they may participate in the biosynthesis of anthocyanins in flesh. These results provide a theoretical basis for further understanding the StPAL gene family and analyzing the function of StPALs in potato.

Key words: potato, PAL genes family, abiotic stress, anthocyanin biosynthesis, expression analysis

ZHU Jin-Yong, LIU Zhen, ZENG Yu-Ting, LI Zhi-Tao, CHEN Li-Min, LI Hong-Yang, SHI Tian-Bin, ZHANG Jun-Lian, BAI Jiang-Ping, LIU Yu-Hui. Genome-wide identification of potato (Solanum tuberosum L.) PAL gene family and its expression analysis in abiotic stress and tuber anthocyanin synthesis[J].Acta Agronomica Sinica, 2023, 49(11): 2978-2990.

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基因名称 Gene name	正向引物 Forward primer (5°-3°)	反向引物 Reverse primer (5°-3°)
StEF-1α	GGTCGTGTTGAGACTGGTGTGATC	GCTTCGTGGTGCATCTCTACAGAC
PG2021549	CTCACAGCAGGAAGGAATCCAAGC	TGAAGTTCCGAGCAGTAAGAAGCC
PG0031457	CTCACAGCAGGAAGGAATCCAAGC	GCTCGGCACTCTGAACATGGTTAG
PG0005492	ATCCGACTAGGTGGTGGTGAGATG	ATCCAATCACTGCTCGCCTTGAC
PG0023458	CACAGCGTCTGGTGACTTGGTAC	GCGTCCAACAGTTCTCCATTAGGC
PG1021549	CTGCTGAGGCTGTGGACATCTTG	TTGGCTACTTGGCTTACGGTGTTC
PG2021564	TGGAACGGTCACTGCCTCAGG	CACTAACACCAGCCACACGGAAC
PG0019386	ACTGCCTCGGGTGATCTTGTCC	ACTAATACCAGCAACACGGAACGC
PG0031365	GCCGAAGGAAGGACTTGCTCTTG	CGGGCTTTCCATTCATCACCTCAG

基因名称 Gene name	氨基酸长度 Amino acid length	相对分子量 Molecular weight (kD)	等电点 Point isoelectric (pI)	亚细胞定位 Subcellular localization	染色体定位 Chromosome localization
PG0031457, StPAL1	435	48.34	5.84	细胞质Cytoplasmic	Chr03
PG0005492, StPAL2	667	73.69	5.41	细胞质Cytoplasmic	Chr05
PG0023458, StPAL3	707	77.51	6.07	细胞质Cytoplasmic	Chr05
PG2021549, StPAL4	391	43.55	6.62	细胞质Cytoplasmic	Chr09
PG1021549, StPAL5	722	78.49	6.04	细胞质Cytoplasmic	Chr09
PG2021564, StPAL6	719	78.23	6.15	细胞质Cytoplasmic	Chr09
PG0019386, StPAL7	689	75.46	6.28	叶绿体Chloroplast	Chr10
PG0031365, StPAL8	711	77.54	5.86	细胞质Cytoplasmic	Chr10

Genome-wide identification of potato (Solanum tuberosum L.) PAL gene family and its expression analysis in abiotic stress and tuber anthocyanin synthesis

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