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Acta Agronomica Sinica ›› 2024, Vol. 50 ›› Issue (3): 779-792.doi: 10.3724/SP.J.1006.2024.31045

• RESEARCH NOTES • Previous Articles    

Genome wide identification and expression analysis of TaPOD family in wheat

JU Ji-Hao1(), MA Chao1, WANG Tian-Ning1, WU Yi1, DONG Zhong2, FANG Mei-E1, CHEN Yu-Shu1, ZHANG Jun1,*(), FU Guo-Zhan1,*()   

  1. 1College of Agriculture, Henan University of Science and Technology, Luoyang 471000, Henan, China
    2College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, Henan, China
  • Received:2023-07-27 Accepted:2023-10-24 Online:2024-03-12 Published:2023-11-10
  • Contact: *E-mail: zhangjun0105@126.com; E-mail: fuguozhan@163.com
  • Supported by:
    National Natural Science Foundation of China(32372227);National Natural Science Foundation of China(31401323);National Natural Science Foundation of Henan Province(222300420430);Training Program for University Young Key Teachers in Henan Province(2021GGJS050);Innovation and Entrepreneurship Training Program for College Students in 2023(2023418);Innovation and Entrepreneurship Training Program for College Students in 2023(2023412)

Abstract:

Peroxidase (POD) family members play the important roles in regulating plant growth, development, and responding to stress. In order to systematically explore the functions and expression patterns of the TaPOD gene family in wheat (Triticum aestivum L.), in this study, we identified the members of the TaPOD gene family in wheat using bioinformatics methods, predicted their physical and chemical properties, promoter homeopathic elements, and evolutionary characteristics, and analyzed their expression patterns in different tissues, exogenous hormones, and stress conditions through wheat transcriptome and RT-qPCR. The results showed that 659 members of the TaPOD gene family were identified from wheat, and their protein sequence lengths were 206-518 amino acids; Phylogenetic analysis showed that the members of wheat TaPOD family were divided into I-VII groups with different numbers of each group. Sequence alignment demonstrated that the members of wheat TaPOD family had five conservative motifs, with significant differences in gene structure, indicating diversification in functions. Chromosome localization revealed uneven distribution of its quantity on 21 chromosomes of wheat, with the highest number on chromosome 2B. The intraspecific collinearity analysis, showed that there were 396 repetitive events in wheat TaPOD genes, with high homology and very conservative evolutionary process. It was amplified mainly through fragment replication and tandem replication, and the Ka/Ks ratio showed that only four pairs of family members were subjected to positive natural selection pressure. The analysis of cis-regulatory element showed that there were 23 binding elements related to growth, development, and stress resistance in the upstream 2 kb region. Analysis of gene expression patterns showed that most TaPOD genes were highly expressed in wheat roots, and the relative expression level was closely related to hormone induction and abiotic stress. The above results provide a preliminary theoretical basis for further understanding the functions of TaPOD genes in regulating wheat growth, development, and stress.

Key words: wheat, TaPOD gene family, bioinformatics, collinearity analysis, gene expression

Table 1

Partial primers of TaPOD genes for RT-qPCR"

登录号
Accession number
基因名称
Gene name
上游引物
Forward primer (5'-3')
下游引物
Reverse primer (5'-3')
TraesCS7A02G211200.1 TaPOD534 TGGCACAGGACCCTTCAAAT GCACCGCACACTCCATCTCT
TraesCS7B02G118200.1 TaPOD570 CAATGCCGCTTTGATGCTC TTCTGGTCTGCCTGCTTCTCA
TraesCS7D02G212900.1 TaPOD615 GGCAGACCAGAATGTGAACTCCT TGCGTCCCTGGCTGCG
TraesCS2D02G080000.1 TaPOD228 AGACAGGACAAGCCCGAGC CCACCAGAAAGAGCAACAATG
TraesCS1A02G079400.1 TaPOD8 GCACCTCACACTGCTCATCGTTC ACGGCGGACACTTCTTCTTGAG
TraesCS2D02G233600.1 TaPOD256 TGTCGCTTCTCTCCTTCCTCG CAAAGTCGTGGAAGAAGAGGCA
TraesCS5D02G144300.1 TaPOD458 GCAACACACGCACGCTCTAT CCACGGCTGACTGCACCAC
TraesCS3D02G031800.1 TaPOD340 AGGGCTGGAGCAAATCGTG GCGTAAAGCAGTCGTGGAAGA
TraesCS1A02G318600.1 TaPOD26 TCTACCACCCCCGACCTCC CTTGCTCTTTAGCCGCATCAT
TraesCS1B02G283900.1 Actin GTTCCAATCTATGAGGGATACACGC GAACCTCCACTGAGAACAACATTACC

Table S1

Members and physicochemical properties of TaPOD protein family"

登录号
Accession number
基因名称
Gene name
氨基酸数量
Number of amino acid
分子量
Molecular weight
理论等电点
Theoretical pI
不稳定指数
Instability index
脂肪指数
Aliphatic index
水文性指数
Grand Average of hydropathicity
TraesCS1A02G077700.2 TaPOD1 339 36737.13 6.67 30.60 96.19 −0.03
TraesCS1A02G077900.1 TaPOD2 311 33369.67 6.39 23.97 83.12 −0.28
TraesCS1A02G078000.1 TaPOD3 347 37316.05 7.71 28.51 82.19 −0.25
TraesCS1A02G078100.1 TaPOD4 338 35683.76 8.47 33.27 88.08 0.04
TraesCS1A02G078200.1 TaPOD5 341 36732.43 5.38 38.14 82.43 −0.10
TraesCS1A02G078400.1 TaPOD6 341 36809.73 7.66 31.43 75.87 −0.16
TraesCS1A02G078600.1 TaPOD7 341 36733.60 6.79 28.07 77.60 −0.16
TraesCS1A02G079400.1 TaPOD8 343 37179.27 5.25 35.86 79.68 −0.14
TraesCS1A02G079500.1 TaPOD9 341 36832.91 8.77 32.76 79.30 −0.17
TraesCS1A02G104100.1 TaPOD10 331 35230.98 5.52 25.06 86.89 −0.01
TraesCS1A02G104200.1 TaPOD11 331 35507.13 4.79 28.09 86.01 −0.04
TraesCS1A02G104300.1 TaPOD12 280 29621.57 5.49 21.33 87.89 0.03
TraesCS1A02G108400.1 TaPOD13 362 38149.25 6.70 38.25 87.90 0.09
TraesCS1A02G124200.1 TaPOD14 348 37581.39 4.86 48.45 87.27 −0.02
TraesCS1A02G200100.1 TaPOD15 320 34782.49 9.23 37.94 79.00 −0.20
TraesCS1A02G200200.1 TaPOD16 334 34408.99 6.88 35.70 82.40 0.07
TraesCS1A02G214500.1 TaPOD17 327 35086.21 5.49 40.36 83.91 0.17
TraesCS1A02G304500.1 TaPOD18 325 34804.17 8.62 37.40 88.89 0.04
TraesCS1A02G304600.1 TaPOD19 325 35017.38 8.63 36.89 87.08 0.04
TraesCS1A02G317900.1 TaPOD20 326 35666.77 8.04 34.72 79.02 −0.14
TraesCS1A02G318000.1 TaPOD21 326 35687.76 6.45 33.23 79.63 −0.10
TraesCS1A02G318200.1 TaPOD22 327 35588.67 7.06 29.94 79.11 −0.08
TraesCS1A02G318300.1 TaPOD23 327 35674.78 6.45 32.47 80.31 −0.06
TraesCS1A02G318400.1 TaPOD24 320 34927.89 6.45 29.39 79.28 −0.10
TraesCS1A02G318500.2 TaPOD25 327 35647.75 6.45 31.74 80.31 −0.05
TraesCS1A02G318600.1 TaPOD26 327 35607.68 6.70 30.07 78.81 −0.08
TraesCS1A02G318700.1 TaPOD27 327 35667.72 6.50 30.99 79.69 −0.08
TraesCS1A02G318900.1 TaPOD28 326 35559.58 6.25 34.37 79.94 −0.07
TraesCS1A02G319000.1 TaPOD29 337 36803.86 6.76 47.18 82.28 −0.13
TraesCS1A02G342000.1 TaPOD30 332 35512.77 5.82 34.05 85.84 0.04
TraesCS1A02G342300.1 TaPOD31 332 35634.93 5.89 34.26 85.24 0.01
TraesCS1A02G342400.1 TaPOD32 291 31113.88 6.52 37.37 86.53 0.06
TraesCS1A02G356100.1 TaPOD33 324 34598.78 8.37 38.15 91.05 0.02
TraesCS1B02G095800.1 TaPOD34 340 36893.18 6.39 30.01 95.35 −0.05
TraesCS1B02G096000.1 TaPOD35 338 36885.12 7.59 25.71 83.28 −0.20
TraesCS1B02G096100.1 TaPOD36 347 37611.37 6.00 18.69 84.38 −0.20
TraesCS1B02G096200.1 TaPOD37 338 35746.92 8.76 32.11 87.78 0.03
TraesCS1B02G096300.1 TaPOD38 341 36736.48 5.52 43.35 80.15 −0.10
TraesCS1B02G096400.1 TaPOD39 341 36745.76 7.07 28.26 80.15 −0.11
TraesCS1B02G096600.1 TaPOD40 341 36890.86 7.10 34.46 76.72 −0.16
TraesCS1B02G096800.1 TaPOD41 341 36825.85 7.64 35.03 78.18 −0.16
TraesCS1B02G096900.1 TaPOD42 343 37240.22 5.01 34.34 79.97 −0.13
TraesCS1B02G115000.1 TaPOD43 331 35503.45 5.80 26.46 87.19 0.00
TraesCS1B02G115700.1 TaPOD44 331 35282.20 5.37 26.86 89.24 0.05
TraesCS1B02G115800.1 TaPOD45 331 35434.04 4.78 30.32 86.01 −0.03
TraesCS1B02G115900.1 TaPOD46 342 36572.37 5.57 28.89 83.07 −0.04
TraesCS1B02G142800.1 TaPOD47 375 40493.83 5.20 47.74 86.16 −0.04
TraesCS1B02G142800.2 TaPOD48 348 37506.32 4.78 48.06 87.53 0.00
TraesCS1B02G182900.1 TaPOD49 286 30349.69 8.51 38.94 92.83 0.05
TraesCS1B02G214800.1 TaPOD50 320 34790.52 8.89 38.29 79.91 −0.17
TraesCS1B02G214900.1 TaPOD51 332 34360.84 6.94 35.66 82.02 0.03
TraesCS1B02G228400.1 TaPOD52 327 35144.30 5.61 39.24 86.85 0.17
TraesCS1B02G315100.1 TaPOD53 325 34911.19 8.62 38.61 86.15 0.01
TraesCS1B02G330200.1 TaPOD54 386 41893.84 7.57 43.94 78.65 −0.10
TraesCS1B02G330400.1 TaPOD55 359 39405.12 8.43 35.30 79.08 −0.09
TraesCS1B02G330500.1 TaPOD56 327 35589.65 6.45 27.53 79.11 −0.07
TraesCS1B02G330600.1 TaPOD57 327 35649.75 6.65 27.53 79.11 −0.09
TraesCS1B02G330700.1 TaPOD58 304 33127.93 6.44 29.95 81.88 −0.02
TraesCS1B02G330800.1 TaPOD59 356 39315.97 7.58 31.63 81.15 −0.09
TraesCS1B02G331000.1 TaPOD60 297 32714.24 7.10 24.73 75.89 −0.18
TraesCS1B02G331200.1 TaPOD61 329 35852.94 6.25 31.12 82.19 −0.03
TraesCS1B02G331300.1 TaPOD62 332 36408.49 6.56 47.07 80.27 −0.15
TraesCS1B02G354800.1 TaPOD63 332 35587.88 5.99 34.61 84.94 0.02
TraesCS1B02G354900.1 TaPOD64 335 null null 35.59 84.78 0.05
TraesCS1B02G355000.1 TaPOD65 332 35487.76 5.98 34.35 84.94 0.03
TraesCS1B02G355200.1 TaPOD66 332 35755.00 5.43 36.20 86.45 0.02
TraesCS1B02G365300.1 TaPOD67 326 34781.01 8.56 35.07 89.60 0.01
TraesCS1D02G079800.1 TaPOD68 340 36897.20 6.56 28.77 96.21 −0.04
TraesCS1D02G080000.1 TaPOD69 328 35929.02 7.60 28.89 85.30 −0.18
TraesCS1D02G080100.1 TaPOD70 346 37332.12 7.68 23.20 81.56 −0.21
TraesCS1D02G080200.1 TaPOD71 334 35275.26 6.21 34.17 78.92 0.09
TraesCS1D02G080300.1 TaPOD72 340 35885.00 8.62 32.16 87.00 0.02
TraesCS1D02G080400.1 TaPOD73 341 36609.32 5.73 38.73 80.12 −0.11
TraesCS1D02G080600.1 TaPOD74 341 36755.68 7.63 31.70 77.57 −0.17
TraesCS1D02G080800.1 TaPOD75 341 36808.76 8.11 30.98 77.89 −0.17
TraesCS1D02G080900.1 TaPOD76 331 36036.89 5.02 31.99 82.27 −0.15
TraesCS1D02G096200.1 TaPOD77 331 35287.17 5.80 28.68 87.49 0.03
TraesCS1D02G096300.1 TaPOD78 331 35537.16 4.79 27.01 85.41 −0.05
TraesCS1D02G096400.1 TaPOD79 340 36329.04 5.76 28.58 81.26 −0.07
TraesCS1D02G130200.1 TaPOD80 348 37449.22 4.78 47.68 87.27 0.00
TraesCS1D02G174200.1 TaPOD81 330 34953.69 6.98 40.73 83.45 0.01
TraesCS1D02G203700.1 TaPOD82 331 34196.69 6.88 36.09 81.39 0.04
TraesCS1D02G217500.1 TaPOD83 327 35036.09 5.49 42.44 83.03 0.17
TraesCS1D02G304100.1 TaPOD84 325 34808.12 8.62 38.66 86.18 0.02
TraesCS1D02G304300.1 TaPOD85 325 34883.18 8.62 36.20 86.46 0.01
TraesCS1D02G304400.1 TaPOD86 325 34929.23 8.63 36.46 85.26 0.00
TraesCS1D02G304500.1 TaPOD87 325 34901.22 8.62 36.46 85.26 0.00
TraesCS1D02G317700.1 TaPOD88 326 35756.91 8.04 32.93 78.44 −0.15
TraesCS1D02G317800.1 TaPOD89 327 35689.77 6.70 31.06 78.50 −0.10
TraesCS1D02G317900.1 TaPOD90 327 35648.76 7.58 29.44 78.50 −0.09
TraesCS1D02G318000.1 TaPOD91 327 35635.74 6.45 30.69 79.39 −0.06
TraesCS1D02G318100.1 TaPOD92 327 35689.77 6.70 31.06 78.50 −0.10
TraesCS1D02G318200.1 TaPOD93 327 35689.77 6.70 31.06 78.50 −0.10
TraesCS1D02G318300.1 TaPOD94 316 34369.19 6.17 30.65 78.48 −0.06
TraesCS1D02G318400.1 TaPOD95 283 30940.25 6.65 26.51 77.24 −0.14
TraesCS1D02G318500.1 TaPOD96 265 29043.19 7.07 28.37 76.98 −0.10
TraesCS1D02G318600.1 TaPOD97 327 35680.78 6.70 30.55 79.11 −0.07
TraesCS1D02G318700.1 TaPOD98 326 35600.68 6.45 33.02 79.94 −0.08
TraesCS1D02G318800.1 TaPOD99 335 36682.80 7.07 49.07 80.42 −0.16
TraesCS1D02G344300.1 TaPOD100 332 35586.91 6.17 35.88 84.94 0.02
TraesCS1D02G344500.1 TaPOD101 331 35465.84 6.59 34.04 85.20 0.01
TraesCS1D02G354100.1 TaPOD102 324 34839.07 8.06 35.43 90.71 −0.02
TraesCS1D02G402200.1 TaPOD103 246 26286.48 4.78 24.15 74.59 −0.19
TraesCS2A02G037700.1 TaPOD104 344 37074.34 6.45 48.75 83.81 0.02
TraesCS2A02G071700.1 TaPOD105 295 32360.93 8.25 44.14 86.71 −0.23
TraesCS2A02G082100.1 TaPOD106 408 44323.32 6.46 52.75 73.55 −0.29
TraesCS2A02G083900.1 TaPOD107 384 41509.30 8.63 39.68 79.90 −0.15
TraesCS2A02G084100.1 TaPOD108 384 41509.30 8.63 39.68 79.90 −0.15
TraesCS2A02G106700.1 TaPOD109 321 33842.90 4.23 26.65 91.28 0.05
TraesCS2A02G107200.1 TaPOD110 313 31876.84 6.87 34.64 84.03 0.12
TraesCS2A02G107300.1 TaPOD111 335 35513.44 9.32 43.91 86.00 0.07
TraesCS2A02G107500.1 TaPOD112 339 35926.52 6.98 34.22 89.26 0.04
TraesCS2A02G107600.1 TaPOD113 314 32619.75 8.64 30.11 83.38 0.03
TraesCS2A02G107700.1 TaPOD114 319 33695.03 6.19 36.55 86.33 0.03
TraesCS2A02G107800.1 TaPOD115 314 32580.79 8.38 30.17 83.69 0.06
TraesCS2A02G108000.1 TaPOD116 316 32785.06 5.75 40.02 84.15 0.14
TraesCS2A02G108100.1 TaPOD117 316 33176.23 6.06 33.93 78.48 −0.04
TraesCS2A02G108200.1 TaPOD118 351 37174.19 5.97 37.35 86.21 −0.01
TraesCS2A02G108300.1 TaPOD119 325 33849.13 5.79 38.04 86.25 0.01
TraesCS2A02G108500.1 TaPOD120 325 33817.07 5.53 38.99 87.14 0.03
TraesCS2A02G108600.1 TaPOD121 312 32840.01 6.19 33.42 80.74 0.00
TraesCS2A02G108700.1 TaPOD122 321 33617.14 8.77 40.38 84.64 0.09
TraesCS2A02G144900.1 TaPOD123 347 38442.88 6.19 32.89 83.17 −0.16
TraesCS2A02G145000.1 TaPOD124 346 37842.96 5.18 34.62 79.22 −0.23
TraesCS2A02G147600.1 TaPOD125 333 34981.43 5.11 44.92 84.53 −0.04
TraesCS2A02G147700.1 TaPOD126 319 33865.14 9.31 43.81 91.25 0.08
TraesCS2A02G147800.1 TaPOD127 396 42676.82 9.54 65.58 76.99 −0.17
TraesCS2A02G147900.1 TaPOD128 327 35651.38 9.26 34.21 87.13 −0.02
TraesCS2A02G148100.1 TaPOD129 261 28087.20 6.30 45.29 82.95 −0.08
TraesCS2A02G148200.1 TaPOD130 518 53680.19 6.26 81.61 64.13 −0.32
TraesCS2A02G148600.1 TaPOD131 342 37034.43 6.60 35.51 81.32 −0.17
TraesCS2A02G148900.1 TaPOD132 342 36923.19 5.91 33.38 81.02 −0.17
TraesCS2A02G149400.1 TaPOD133 363 39026.20 5.44 41.33 78.57 −0.18
TraesCS2A02G229900.1 TaPOD134 336 37148.44 8.31 39.84 76.73 −0.32
TraesCS2A02G263500.1 TaPOD135 323 33782.46 8.09 35.20 87.43 0.13
TraesCS2A02G301000.1 TaPOD136 341 36798.81 7.20 39.80 84.19 −0.16
TraesCS2A02G305600.1 TaPOD137 345 37186.87 8.56 47.30 69.51 −0.51
TraesCS2A02G305600.2 TaPOD138 346 37315.98 8.26 48.00 69.31 −0.52
TraesCS2A02G333500.1 TaPOD139 336 35753.70 8.78 41.36 84.38 −0.04
TraesCS2A02G355300.1 TaPOD140 488 49424.18 9.50 70.87 71.95 −0.13
TraesCS2A02G355800.1 TaPOD141 330 34237.58 4.62 35.32 92.09 0.17
TraesCS2A02G451400.1 TaPOD142 361 37212.46 6.07 32.95 94.76 0.22
TraesCS2A02G467000.1 TaPOD143 330 34774.54 9.09 43.27 82.94 0.00
TraesCS2A02G476300.1 TaPOD144 274 29975.15 7.58 44.75 77.59 −0.19
TraesCS2A02G476300.2 TaPOD145 335 36916.55 7.03 43.31 90.21 0.01
TraesCS2A02G502100.1 TaPOD146 328 35109.30 8.96 41.97 85.46 0.13
TraesCS2A02G509800.1 TaPOD147 323 34043.22 5.16 30.47 78.33 −0.11
TraesCS2A02G571300.1 TaPOD148 370 39063.21 5.48 48.55 84.03 0.04
TraesCS2A02G571500.1 TaPOD149 341 35460.24 7.57 44.30 85.75 0.14
TraesCS2A02G571600.1 TaPOD150 327 35674.55 5.14 40.80 90.73 −0.03
TraesCS2A02G571700.1 TaPOD151 341 35536.25 8.13 45.28 82.84 0.08
TraesCS2A02G572500.1 TaPOD152 345 37864.28 6.08 32.61 81.54 −0.16
TraesCS2A02G572600.1 TaPOD153 339 36564.46 6.65 43.24 78.61 −0.12
TraesCS2A02G572700.1 TaPOD154 341 37400.16 5.11 49.06 76.69 −0.22
TraesCS2A02G572800.1 TaPOD155 340 37578.95 5.48 48.05 85.79 −0.11
TraesCS2A02G573300.1 TaPOD156 352 37314.92 4.89 48.89 85.43 0.06
TraesCS2A02G573400.1 TaPOD157 339 36235.52 5.79 39.76 92.77 0.15
TraesCS2A02G573500.1 TaPOD158 341 36509.53 5.58 46.07 93.67 0.09
TraesCS2A02G573700.1 TaPOD159 337 36402.72 7.58 41.90 83.50 −0.09
TraesCS2A02G573900.1 TaPOD160 341 36521.38 5.59 46.43 85.66 −0.01
TraesCS2A02G574400.1 TaPOD161 346 37263.55 6.45 39.26 83.82 −0.04
TraesCS2B02G087400.1 TaPOD162 297 32376.89 8.24 41.36 86.16 −0.23
TraesCS2B02G096200.1 TaPOD163 254 27425.13 5.18 36.95 79.65 −0.19
TraesCS2B02G098100.1 TaPOD164 332 35653.59 8.30 34.72 82.71 −0.13
TraesCS2B02G098200.1 TaPOD165 318 34244.10 8.56 35.81 85.72 −0.13
TraesCS2B02G098400.1 TaPOD166 338 36240.30 8.01 32.42 84.11 −0.12
TraesCS2B02G098600.1 TaPOD167 341 36537.43 8.02 38.71 80.53 −0.13
TraesCS2B02G098700.1 TaPOD168 336 36180.19 8.54 36.06 83.15 −0.17
TraesCS2B02G124300.1 TaPOD169 321 34116.06 4.20 27.36 89.72 −0.05
TraesCS2B02G124600.1 TaPOD170 314 33067.27 6.41 31.91 78.98 −0.01
TraesCS2B02G124800.1 TaPOD171 316 32904.96 5.78 34.72 83.20 0.05
TraesCS2B02G124900.1 TaPOD172 319 33594.13 8.95 41.50 85.14 0.02
TraesCS2B02G125200.1 TaPOD173 339 35831.43 6.64 35.63 87.32 0.03
TraesCS2B02G125200.2 TaPOD174 312 32727.83 5.78 35.13 83.94 0.04
TraesCS2B02G125300.1 TaPOD175 312 32222.37 8.59 28.70 83.30 0.07
TraesCS2B02G125500.1 TaPOD176 316 33062.14 5.77 32.00 79.43 0.00
TraesCS2B02G125600.1 TaPOD177 316 33062.14 5.77 32.00 79.43 0.00
TraesCS2B02G125700.1 TaPOD178 330 34428.84 5.81 33.66 85.21 0.05
TraesCS2B02G125800.1 TaPOD179 314 33090.36 5.78 33.13 80.83 0.01
TraesCS2B02G125900.1 TaPOD180 321 33634.20 8.76 41.25 86.45 0.09
TraesCS2B02G126100.1 TaPOD181 325 33784.05 5.79 39.67 86.25 0.01
TraesCS2B02G126200.1 TaPOD182 314 33074.36 6.41 32.79 80.86 0.01
TraesCS2B02G126300.1 TaPOD183 321 33565.12 8.93 42.20 86.17 0.10
TraesCS2B02G169900.1 TaPOD184 356 38865.21 6.08 34.27 82.19 −0.24
TraesCS2B02G173000.1 TaPOD185 338 35612.12 5.13 44.39 82.43 −0.07
TraesCS2B02G173200.1 TaPOD186 319 33957.16 9.34 42.02 89.09 0.06
TraesCS2B02G173300.1 TaPOD187 396 42822.06 9.60 63.45 77.70 −0.17
TraesCS2B02G173400.1 TaPOD188 327 35359.96 9.23 40.07 86.48 −0.03
TraesCS2B02G173500.1 TaPOD189 505 52338.72 6.18 82.99 62.50 −0.33
TraesCS2B02G173600.1 TaPOD190 342 37318.72 6.25 31.79 78.45 −0.23
TraesCS2B02G173800.1 TaPOD191 342 37318.72 6.25 31.79 78.45 −0.23
TraesCS2B02G173900.1 TaPOD192 342 37185.54 6.24 32.61 78.19 −0.22
TraesCS2B02G174300.1 TaPOD193 363 38826.86 5.31 41.46 79.12 −0.18
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TraesCSU02G134300.1 TaPOD654 330 35814.84 9.35 43.36 88.09 −0.05
TraesCSU02G137000.1 TaPOD655 369 38786.04 6.79 26.04 76.78 −0.09
TraesCSU02G137300.1 TaPOD656 423 41965.72 5.72 34.12 66.55 −0.14
TraesCSU02G151100.1 TaPOD657 329 35554.91 8.04 27.64 87.26 −0.07
TraesCSU02G163200.1 TaPOD658 329 35618.89 6.54 31.95 88.45 −0.07
TraesCSU02G250800.1 TaPOD659 330 35224.03 4.57 37.55 89.30 0.05

Fig. 1

Identification of conserved sequence of TaPOD family members in wheat"

Fig. 2

Phylogenetic relationships among members of TaPOD family in wheat"

Fig. 3

Analysis of the phylogenetic relationship and gene structure of TaPOD gene in wheat A: the phylogenetic development of TaPOD protein. The Neighbor joining method was used to construct the phylogenetic tree, and different colors represented different groups. B: conservative motif analysis of TaPOD protein. Each pattern consists of different colored squares and numbers. C: structural analysis of TaPOD gene. The exon, intron, and UTR are represented by green box, black line, and yellow box, respectively."

Fig. 4

Chromosomal distribution of TaPOD gene in wheat The black line represents tandem replication between genes. The depth of band color in chromosomes indicates the size of gene density."

Fig. 5

Regional collinearity of TaPOD gene in wheat The homologous chromosome regions were connected with TaPOD genes by red lines. Ring 1 is the gene density heatmap. The points and ines of rings 2 and 3 indicate the location of base deletions."

Table S2

Ka/Ks of repeated events in wheat TaPOD"

序列1
Seq_1
序列2
Seq_2
非同义替换率
Ka
同义替换率
Ks
非同义替换/同义替换
Ka/Ks
有效长度
Effective length
平均S位点
Average S-sites
平均N位点
Average N-sites
cN cS pN pS
TraesCS1A02G077700.2 TraesCS1B02G095800.1 0.0079 0.0712 0.1105 1017 250.3333 766.6667 6.0000 17.0000 0.0078 0.0679
TraesCS1A02G077700.2 TraesCS1D02G079800.1 0.0092 0.0580 0.1586 1017 251.0000 766.0000 7.0000 14.0000 0.0091 0.0558
TraesCS1A02G077900.1 TraesCS1B02G096000.1 0.4176 0.5636 0.7409 828 195.5833 632.4167 202.5000 77.5000 0.3202 0.3963
TraesCS1A02G077900.1 TraesCS1D02G080000.1 0.4324 0.5289 0.8175 825 194.3333 630.6667 207.2500 73.7500 0.3286 0.3795
TraesCS1A02G078000.1 TraesCS1B02G096100.1 0.0695 0.1872 0.3714 1038 252.5000 785.5000 52.1667 41.8333 0.0664 0.1657
TraesCS1A02G078100.1 TraesCS1B02G096200.1 0.0175 0.0595 0.2943 1014 262.4167 751.5833 13.0000 15.0000 0.0173 0.0572
TraesCS1A02G078100.1 TraesCS1D02G080200.1 0.4747 0.7871 0.6032 969 244.5000 724.5000 254.8333 119.1667 0.3517 0.4874
TraesCS1A02G078200.1 TraesCS1B02G096300.1 0.0306 0.2064 0.1480 1023 254.9167 768.0833 23.0000 46.0000 0.0299 0.1805
TraesCS1A02G078200.1 TraesCS1D02G080400.1 0.0251 0.2118 0.1187 1023 254.6667 768.3333 19.0000 47.0000 0.0247 0.1846
TraesCS1A02G104100.1 TraesCS1B02G115700.1 0.0279 0.0939 0.2969 993 243.6667 749.3333 20.5000 21.5000 0.0274 0.0882
TraesCS1A02G104100.1 TraesCS1D02G096200.1 0.0189 0.0688 0.2747 993 243.2500 749.7500 14.0000 16.0000 0.0187 0.0658
TraesCS1A02G104300.1 TraesCS1B02G115900.1 0.0534 0.1830 0.2919 834 216.5833 617.4167 31.8333 35.1667 0.0516 0.1624
TraesCS1A02G104300.1 TraesCS1D02G096400.1 0.0429 0.1223 0.3510 831 215.6667 615.3333 25.6667 24.3333 0.0417 0.1128
TraesCS1A02G108400.1 TraesCS1D02G110600.1 0.0435 0.0852 0.5105 669 175.9167 493.0833 20.8333 14.1667 0.0423 0.0805
TraesCS1A02G124200.1 TraesCS1B02G142800.1 0.0102 0.0534 0.1907 1044 252.4167 791.5833 8.0000 13.0000 0.0101 0.0515
TraesCS1A02G124200.1 TraesCS1D02G130200.1 0.0102 0.0489 0.2081 1044 253.2500 790.7500 8.0000 12.0000 0.0101 0.0474
TraesCS1A02G200100.1 TraesCS1D02G203700.1 0.5001 0.5686 0.8794 945 240.8333 704.1667 257.0000 96.0000 0.3650 0.3986
TraesCS1A02G200100.1 TraesCS4B02G347600.2 0.4690 0.5993 0.7827 945 241.9167 703.0833 245.1667 99.8333 0.3487 0.4127
TraesCS1A02G200100.1 TraesCS4D02G342500.1 0.4556 0.6002 0.7591 945 241.6667 703.3333 240.1667 99.8333 0.3415 0.4131
TraesCS1A02G200100.1 TraesCS5A02G516200.1 0.4590 0.5704 0.8047 945 241.5833 703.4167 241.5000 96.5000 0.3433 0.3994
TraesCS1A02G214500.1 TraesCS1D02G217500.1 0.0143 0.0633 0.2256 981 238.8333 742.1667 10.5000 14.5000 0.0141 0.0607
TraesCS1A02G317900.1 TraesCS1B02G330200.1 0.0741 0.2234 0.3317 978 236.3333 741.6667 52.3333 45.6667 0.0706 0.1932
TraesCS1A02G317900.1 TraesCS1D02G317700.1 0.0054 0.0669 0.0808 978 234.5000 743.5000 4.0000 15.0000 0.0054 0.0640
TraesCS1A02G318300.1 TraesCS1B02G330600.1 0.0143 0.0545 0.2619 981 237.9167 743.0833 10.5000 12.5000 0.0141 0.0525
TraesCS1A02G318900.1 TraesCS1D02G318600.1 0.0219 0.1815 0.1205 978 235.7500 742.2500 16.0000 38.0000 0.0216 0.1612
TraesCS1A02G319000.1 TraesCS1B02G331300.1 0.0261 0.0787 0.3311 996 254.2500 741.7500 19.0000 19.0000 0.0256 0.0747
TraesCS1A02G319000.1 TraesCS1D02G318800.1 0.0183 0.0335 0.5468 1005 259.3333 745.6667 13.5000 8.5000 0.0181 0.0328
TraesCS1A02G319000.1 TraesCS3A02G325100.2 0.3604 0.5741 0.6279 981 245.7500 735.2500 210.4167 98.5833 0.2862 0.4012
TraesCS1A02G319000.1 TraesCS3B02G354000.1 0.3806 0.5488 0.6935 981 244.7500 736.2500 219.7500 95.2500 0.2985 0.3892
TraesCS1A02G319000.1 TraesCS3D02G318500.1 0.3746 0.5211 0.7189 981 244.2500 736.7500 217.2500 91.7500 0.2949 0.3756
TraesCS1A02G342000.1 TraesCS1D02G344300.1 0.0161 0.1354 0.1188 996 242.1667 753.8333 12.0000 30.0000 0.0159 0.1239
TraesCS1A02G356100.1 TraesCS1B02G365300.1 0.0210 0.1034 0.2034 972 248.5833 723.4167 15.0000 24.0000 0.0207 0.0965
TraesCS1A02G356100.1 TraesCS1D02G354100.1 0.0224 0.0854 0.2625 972 247.7500 724.2500 16.0000 20.0000 0.0221 0.0807
TraesCS1B02G095800.1 TraesCS1D02G079800.1 0.0052 0.0326 0.1603 1020 251.0000 769.0000 4.0000 8.0000 0.0052 0.0319
TraesCS1B02G096000.1 TraesCS1D02G080000.1 0.2273 0.1805 1.2592 975 229.5833 745.4167 146.1667 36.8333 0.1961 0.1604
TraesCS1B02G096200.1 TraesCS1D02G080200.1 0.4872 0.7239 0.6730 966 244.0833 721.9167 258.6667 113.3333 0.3583 0.4643
TraesCS1B02G096300.1 TraesCS1D02G080400.1 0.0118 0.1409 0.0840 1023 256.9167 766.0833 9.0000 33.0000 0.0117 0.1284
TraesCS1B02G115700.1 TraesCS1D02G096200.1 0.0231 0.0686 0.3360 993 244.0833 748.9167 17.0000 16.0000 0.0227 0.0656
TraesCS1B02G115900.1 TraesCS1D02G096400.1 0.0224 0.1048 0.2137 1020 257.2500 762.7500 16.8333 25.1667 0.0221 0.0978
TraesCS1B02G142800.1 TraesCS1D02G130200.1 0.0051 0.0281 0.1811 1044 254.1667 789.8333 4.0000 7.0000 0.0051 0.0275
TraesCS1B02G214800.1 TraesCS1D02G203700.1 0.4879 0.5451 0.8949 918 234.6667 683.3333 245.0833 90.9167 0.3587 0.3874
TraesCS1B02G228400.1 TraesCS1D02G217500.1 0.0212 0.0767 0.2764 981 239.9167 741.0833 15.5000 17.5000 0.0209 0.0729
TraesCS1B02G330200.1 TraesCS1D02G317700.1 0.0733 0.2100 0.3490 978 235.6667 742.3333 51.8333 43.1667 0.0698 0.1832
TraesCS1B02G330700.1 TraesCS1D02G317900.1 0.0131 0.0661 0.1987 912 221.1667 690.8333 9.0000 14.0000 0.0130 0.0633
TraesCS1B02G331000.1 TraesCS1D02G318700.1 0.0715 0.2312 0.3091 885 211.5000 673.5000 45.9167 42.0833 0.0682 0.1990
TraesCS1B02G331300.1 TraesCS1D02G318800.1 0.0192 0.0705 0.2716 993 252.5833 740.4167 14.0000 17.0000 0.0189 0.0673
TraesCS1B02G331300.1 TraesCS3A02G325100.2 0.3386 0.5532 0.6121 981 241.5000 739.5000 201.5000 94.5000 0.2725 0.3913
TraesCS1B02G331300.1 TraesCS3B02G354000.1 0.3677 0.5505 0.6679 990 244.0000 746.0000 216.8333 95.1667 0.2907 0.3900
TraesCS1B02G331300.1 TraesCS3D02G318500.1 0.3609 0.5269 0.6849 990 243.5000 746.5000 213.8333 92.1667 0.2864 0.3785
TraesCS1B02G365300.1 TraesCS1D02G354100.1 0.0153 0.0588 0.2608 972 247.5000 724.5000 11.0000 14.0000 0.0152 0.0566
TraesCS1D02G203700.1 TraesCS4B02G347600.2 0.3544 0.5175 0.6849 963 251.9167 711.0833 200.8333 94.1667 0.2824 0.3738
TraesCS1D02G203700.1 TraesCS4D02G342500.1 0.3420 0.5367 0.6372 957 250.0000 707.0000 194.1667 95.8333 0.2746 0.3833
TraesCS1D02G203700.1 TraesCS5A02G516200.1 0.3426 0.5113 0.6701 960 250.6667 709.3333 195.0833 92.9167 0.2750 0.3707
TraesCS1D02G318800.1 TraesCS3A02G325100.2 0.3510 0.5633 0.6232 993 247.4167 745.5833 209.0000 98.0000 0.2803 0.3961
TraesCS1D02G318800.1 TraesCS3B02G354000.1 0.3769 0.5911 0.6376 993 246.7500 746.2500 221.0833 100.9167 0.2963 0.4090
TraesCS1D02G318800.1 TraesCS3D02G318500.1 0.3689 0.5622 0.6561 993 246.2500 746.7500 217.5833 97.4167 0.2914 0.3956
TraesCS2A02G037700.1 TraesCS2D02G036700.1 0.0264 0.0515 0.5128 993 261.0833 731.9167 19.0000 13.0000 0.0260 0.0498
TraesCS2A02G071700.1 TraesCS2D02G070500.1 0.0045 0.1888 0.0240 885 221.6667 663.3333 3.0000 37.0000 0.0045 0.1669
TraesCS2A02G082100.1 TraesCS2B02G096200.1 0.0052 0.0851 0.0615 762 186.5000 575.5000 3.0000 15.0000 0.0052 0.0804
TraesCS2A02G082100.1 TraesCS2D02G080000.1 0.0017 0.0554 0.0311 768 187.3333 580.6667 1.0000 10.0000 0.0017 0.0534
TraesCS2A02G082100.1 TraesCS4A02G106300.2 0.1157 0.5858 0.1974 750 182.4167 567.5833 60.8333 74.1667 0.1072 0.4066
TraesCS2A02G082100.1 TraesCS4B02G197900.2 0.1119 0.6119 0.1828 684 168.3333 515.6667 53.5833 70.4167 0.1039 0.4183
TraesCS2A02G082100.1 TraesCS4D02G198500.1 0.1157 0.6594 0.1755 750 182.7500 567.2500 60.8333 80.1667 0.1072 0.4387
TraesCS2A02G083900.1 TraesCS2B02G098100.1 0.0200 0.1309 0.1529 996 262.1667 733.8333 14.5000 31.5000 0.0198 0.1202
TraesCS2A02G083900.1 TraesCS2D02G081900.1 0.0273 0.1069 0.2554 1017 271.0000 746.0000 20.0000 27.0000 0.0268 0.0996
TraesCS2A02G106700.1 TraesCS2B02G124300.1 0.0228 0.1487 0.1536 963 252.0000 711.0000 16.0000 34.0000 0.0225 0.1349
TraesCS2A02G106700.1 TraesCS5A02G148600.1 0.4156 0.6880 0.6041 939 239.0833 699.9167 223.3333 107.6667 0.3191 0.4503
TraesCS2A02G106700.1 TraesCS5B02G147200.2 0.4265 0.6938 0.6147 939 240.0833 698.9167 227.3333 108.6667 0.3253 0.4526
TraesCS2A02G106700.1 TraesCS5D02G144300.1 0.4088 0.7167 0.5704 939 239.5833 699.4167 220.4167 110.5833 0.3151 0.4616
TraesCS2A02G107200.1 TraesCS2B02G124400.1 0.1083 0.2359 0.4590 303 83.1667 219.8333 22.1667 16.8333 0.1008 0.2024
TraesCS2A02G107200.1 TraesCS2D02G107400.1 0.0163 0.0982 0.1663 939 248.0000 691.0000 11.1667 22.8333 0.0162 0.0921
TraesCS2A02G107300.1 TraesCS2B02G124500.1 0.0375 0.2287 0.1638 660 167.4167 492.5833 18.0000 33.0000 0.0365 0.1971
TraesCS2A02G107300.1 TraesCS2D02G107500.1 0.0248 0.1469 0.1686 945 247.3333 697.6667 17.0000 33.0000 0.0244 0.1334
TraesCS2A02G107500.1 TraesCS2B02G124800.1 0.0715 0.3146 0.2272 936 239.3333 696.6667 47.5000 61.5000 0.0682 0.2570
TraesCS2A02G107700.1 TraesCS2D02G107800.1 0.0616 0.1889 0.3264 936 237.5833 698.4167 41.3333 39.6667 0.0592 0.1670
TraesCS2A02G108000.1 TraesCS2B02G125500.1 0.0762 0.3449 0.2208 948 236.9167 711.0833 51.5000 65.5000 0.0724 0.2765
TraesCS2A02G108000.1 TraesCS2D02G108100.1 0.0163 0.1945 0.0840 948 236.4167 711.5833 11.5000 40.5000 0.0162 0.1713
TraesCS2A02G108100.1 TraesCS4B02G168000.1 0.2458 0.8632 0.2848 924 235.3333 688.6667 144.3333 120.6667 0.2096 0.5127
TraesCS2A02G108200.1 TraesCS2B02G125700.1 0.1232 0.1387 0.8882 984 244.7500 739.2500 84.0000 31.0000 0.1136 0.1267
TraesCS2A02G108200.1 TraesCS2D02G108300.1 0.1265 0.1482 0.8539 984 245.4167 738.5833 86.0000 33.0000 0.1164 0.1345
TraesCS2A02G108600.1 TraesCS2B02G125800.1 0.0254 0.1275 0.1990 936 234.5000 701.5000 17.5000 27.5000 0.0249 0.1173
TraesCS2A02G108600.1 TraesCS2D02G108500.1 0.0100 0.1408 0.0713 936 233.6667 702.3333 7.0000 30.0000 0.0100 0.1284
TraesCS2A02G144900.1 TraesCS2B02G169900.1 0.1247 0.2273 0.5486 1023 239.6667 783.3333 90.0000 47.0000 0.1149 0.1961
TraesCS2A02G144900.1 TraesCS2D02G148800.1 0.1373 0.2463 0.5573 1005 235.7500 769.2500 96.5000 49.5000 0.1254 0.2100
TraesCS2A02G147600.1 TraesCS2B02G173000.1 0.0248 0.1065 0.2331 999 261.8333 737.1667 18.0000 26.0000 0.0244 0.0993
TraesCS2A02G147600.1 TraesCS2D02G152400.1 0.0380 0.0773 0.4916 987 258.5833 728.4167 27.0000 19.0000 0.0371 0.0735
TraesCS2A02G147800.1 TraesCS2B02G173300.1 0.0079 0.1513 0.0523 1188 299.1667 888.8333 7.0000 41.0000 0.0079 0.1370
TraesCS2A02G147800.1 TraesCS2D02G152700.1 0.0125 0.1810 0.0689 1188 298.5000 889.5000 11.0000 48.0000 0.0124 0.1608
TraesCS2A02G148200.1 TraesCS2B02G173500.1 0.0615 0.1831 0.3357 1497 373.3333 1123.6667 66.3333 60.6667 0.0590 0.1625
TraesCS2A02G148200.1 TraesCS2D02G153100.1 0.0370 0.1439 0.2568 1524 379.9167 1144.0833 41.2500 49.7500 0.0361 0.1309
TraesCS2A02G149400.1 TraesCS2B02G174300.1 0.0197 0.1825 0.1078 1089 265.4167 823.5833 16.0000 43.0000 0.0194 0.1620
TraesCS2A02G229900.1 TraesCS2B02G251400.1 0.0079 0.0503 0.1574 1008 246.5000 761.5000 6.0000 12.0000 0.0079 0.0487
TraesCS2A02G229900.1 TraesCS2D02G233600.1 0.0053 0.0374 0.1412 1008 246.9167 761.0833 4.0000 9.0000 0.0053 0.0364
TraesCS2A02G263500.1 TraesCS5A02G400500.1 0.3589 0.6087 0.5896 948 247.6667 700.3333 199.7500 103.2500 0.2852 0.4169
TraesCS2A02G263500.1 TraesCS5B02G405300.1 0.3542 0.6090 0.5817 948 247.5833 700.4167 197.7500 103.2500 0.2823 0.4170
TraesCS2A02G263500.1 TraesCS5D02G410500.1 0.3705 0.6179 0.5996 957 250.4167 706.5833 206.5833 105.4167 0.2924 0.4210
TraesCS2A02G301000.1 TraesCS2B02G317200.1 0.0078 0.0363 0.2161 1023 254.0000 769.0000 6.0000 9.0000 0.0078 0.0354
TraesCS2A02G301000.1 TraesCS2D02G299700.1 0.0039 0.0322 0.1212 1023 253.5000 769.5000 3.0000 8.0000 0.0039 0.0316
TraesCS2A02G305600.2 TraesCS2B02G322400.1 0.0110 0.0421 0.2599 1038 256.2500 781.7500 8.5000 10.5000 0.0109 0.0410
TraesCS2A02G305600.2 TraesCS2D02G304100.1 0.0129 0.0871 0.1480 1038 255.4167 782.5833 10.0000 21.0000 0.0128 0.0822
TraesCS2A02G355300.1 TraesCS2B02G373000.1 0.0224 0.1237 0.1811 1446 403.5000 1042.5000 23.0000 46.0000 0.0221 0.1140
TraesCS2A02G355300.1 TraesCS2D02G353000.1 0.0149 0.1151 0.1291 1413 393.5833 1019.4167 15.0000 42.0000 0.0147 0.1067
TraesCS2A02G451400.1 TraesCS2B02G472900.1 0.0229 0.0975 0.2347 1083 284.3333 798.6667 18.0000 26.0000 0.0225 0.0914
TraesCS2A02G451400.1 TraesCS2D02G451200.1 0.0229 0.0622 0.3679 1083 284.6667 798.3333 18.0000 17.0000 0.0225 0.0597
TraesCS2A02G476300.2 TraesCS2B02G499900.2 0.0148 0.0738 0.2011 1005 256.1667 748.8333 11.0000 18.0000 0.0147 0.0703
TraesCS2A02G476300.2 TraesCS2D02G476200.2 0.0318 0.1396 0.2281 1005 256.5000 748.5000 23.3333 32.6667 0.0312 0.1274
TraesCS2A02G502100.1 TraesCS2B02G530200.1 0.0069 0.0533 0.1290 984 252.8333 731.1667 5.0000 13.0000 0.0068 0.0514
TraesCS2A02G502100.1 TraesCS2D02G502600.1 0.0180 0.0660 0.2730 984 253.4167 730.5833 13.0000 16.0000 0.0178 0.0631
TraesCS2A02G509800.1 TraesCS2B02G537700.1 0.0125 0.1007 0.1244 969 244.1667 724.8333 9.0000 23.0000 0.0124 0.0942
TraesCS2A02G509800.1 TraesCS2D02G511200.1 0.0097 0.0910 0.1069 969 245.0000 724.0000 7.0000 21.0000 0.0097 0.0857
TraesCS2A02G571600.1 TraesCS2D02G584000.1 0.3524 0.6763 0.5211 870 202.9167 667.0833 187.5833 90.4167 0.2812 0.4456
TraesCS2A02G572500.1 TraesCS2B02G615000.1 0.4346 0.5624 0.7729 999 234.8333 764.1667 252.0833 92.9167 0.3299 0.3957
TraesCS2A02G572500.1 TraesCS2D02G583700.1 0.1917 0.2869 0.6683 1020 237.3333 782.6667 132.4167 56.5833 0.1692 0.2384
TraesCS2A02G572800.1 TraesCS2D02G584000.1 0.3502 0.9872 0.3548 963 225.0000 738.0000 206.5000 123.5000 0.2798 0.5489
TraesCS2A02G573400.1 TraesCS2D02G584600.1 0.0254 0.1663 0.1525 1017 254.8333 762.1667 19.0000 38.0000 0.0249 0.1491
TraesCS2B02G087400.1 TraesCS2D02G070500.1 0.0152 0.1655 0.0921 885 222.1667 662.8333 10.0000 33.0000 0.0151 0.1485
TraesCS2B02G096200.1 TraesCS2D02G080000.1 0.0035 0.0732 0.0476 762 186.3333 575.6667 2.0000 13.0000 0.0035 0.0698
TraesCS2B02G096200.1 TraesCS4A02G106300.2 0.1106 0.6146 0.1800 750 182.7500 567.2500 58.3333 76.6667 0.1028 0.4195
TraesCS2B02G096200.1 TraesCS4B02G197900.2 0.1052 0.6938 0.1517 684 168.8333 515.1667 50.5833 76.4167 0.0982 0.4526
TraesCS2B02G096200.1 TraesCS4D02G198500.1 0.1107 0.7334 0.1509 750 183.0833 566.9167 58.3333 85.6667 0.1029 0.4679
TraesCS2B02G098100.1 TraesCS2D02G081900.1 0.0385 0.0822 0.4681 996 263.1667 732.8333 27.5000 20.5000 0.0375 0.0779
TraesCS2B02G124300.1 TraesCS2D02G107300.1 0.0199 0.1066 0.1870 963 251.5000 711.5000 14.0000 25.0000 0.0197 0.0994
TraesCS2B02G124300.1 TraesCS4B02G168100.1 0.3651 0.6596 0.5535 945 246.1667 698.8333 202.0000 108.0000 0.2891 0.4387
TraesCS2B02G124300.1 TraesCS5A02G148600.1 0.4233 0.7414 0.5709 939 239.2500 699.7500 226.3333 112.6667 0.3234 0.4709
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TraesCS7B02G353100.1 TraesCS7D02G442200.1 0.0282 0.1760 0.1601 993 251.7500 741.2500 20.5000 39.5000 0.0277 0.1569
TraesCS7B02G375600.1 TraesCS7D02G461200.1 0.0077 0.1106 0.0697 1035 252.7500 782.2500 6.0000 26.0000 0.0077 0.1029
TraesCS7B02G376000.1 TraesCS7D02G461500.1 0.0227 0.1337 0.1700 1092 265.4167 826.5833 18.5000 32.5000 0.0224 0.1224
TraesCS7B02G378700.1 TraesCS7D02G464600.1 0.0100 0.0719 0.1388 954 248.0000 706.0000 7.0000 17.0000 0.0099 0.0685
TraesCS5A02G550100.1 TraesCSU02G127400.1 0.1085 0.2211 0.4909 525 143.1667 381.8333 38.5833 27.4167 0.1010 0.1915

Table 2

Ka/Ks of repeated events in wheat TaPOD (Part)"

序列1
Seq 1
序列2
Seq 2
非同义替换率
Ka
同义替换率
Ks
非同义替换/同义替换
Ka/Ks
有效长度
Effective length
平均S位点
Average S-sites
TaPOD35 TaPOD69 0.2272 0.1805 1.2591 975 229.5833
TaPOD309 TaPOD340 0.0318 0.0176 1.8094 1011 258.3333
TaPOD369 TaPOD415 0.0294 0.0203 1.4460 1155 273.3333
TaPOD397 TaPOD415 0.0277 0.0147 1.8791 1155 273.6667
TaPOD570 TaPOD615 0.0026 0.1103 0.0230 1002 233.8333
TaPOD369 TaPOD397 0.0340 0.0353 0.9637 1161 275.1667

Fig. 6

Cis-acting element of TaPOD gene promoter in wheat Different promoter cis-acting elements are represented by the blocks of different colors."

Fig. 7

Relative expression pattern of wheat TaPOD genes in different tissues"

Fig. 8

Relative expression pattern of TaPOD gene family members under four hormone treatments CK: control; IAA: auxin; ABA: abscisic acid; GA: gibberellin; ZT: zeatin."

Fig. 9

Relative expression pattern of TaPOD gene family members under four stresses"

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