Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2024, Vol. 50 ›› Issue (8): 1920-1933.doi: 10.3724/SP.J.1006.2024.33070

;

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Functional study on the regulation of root growth and development and stress tolerance by maize transcription factor ZmEREB180

LIU Chen-Ming1,2(), ZHAO Ke-Yong2(), YUE Man-Fang2, ZHAO Yan-Ming1,*(), WU Zhong-Yi2,*(), ZHANG Chun2,*()   

  1. 1College of Agriculture, Qingdao Agricultural University, Qingdao 266109, Shandong, China
    2Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences / Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, China
  • Received:2023-11-24 Accepted:2024-04-01 Online:2024-08-12 Published:2024-04-19
  • Contact: * E-mail: zhangchun@babrc.ac.cn;E-mail: zwu22@126.com;E-mail: zhaoym796@sina.com
  • About author:** Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(32001430);National Natural Science Foundation of China(32372053)

RichHTML427

61

PDF

691

Abstract:

The AP2/ERF (APETALA2/ethylene-responsive factor) family is one of the largest families of transcription factors in plants, and plays an important role in regulating a variety of biological processes such as plant growth and development, respond-ing to adversity stress, and regulating hormone signaling and substance metabolism. The biological functions of AP2/ERF family genes in many plant species have been validated, but fewer studies have been reported in maize (Zea mays L.). In the previous work, there was significant difference in the relative expression level of the ZmEREB180 transcription factor in root system between the identified critical developmental stages of maize at the six-leaf (V6), the twelve-leaf (V12), and tasseling (VT) stages, the tissue expression analysis revealed that this gene was mainly expressed in maize root system and was significantly higher in young roots than in mature roots, and it was hypothesized that this gene might be involved in the regulation of maize root growth and development. In this study, we cloned the ZmEREB180 (Gene ID: 100192457) transcription factor gene, and preliminarily analyzed the relative expression pattern and biological functions of ZmEREB180 by bioinformatics, RT-qPCR, subcellular localization, and stress-resistant phenotype identification of transgenic Arabidopsis (Arabidopsis thaliana L.) lines. This gene contained two exons and the full-length cDNA was 1023 bp, encoding 340 amino acids. The gene had a conserved domain unique to the AP2/ERF family, which expressed most highly in root system of maize; and the gene had different degrees of induced expression under high salt, drought, high nitrogen, and low nitrogen treatment conditions, with a more rapid and higher expression in response to low nitrogen than high nitrogen; root length of ZmEREB180 transgenic Arabidopsis lines were all significantly longer than the wild type (WT) on 1/2 MS medium containing 0.10 mol L-1, 0.15 mol L-1 NaCl, and 0.15 mol L-1, 0.20 mol L-1, 0.30 mol L-1 mannitol (MNT). Under high salt and drought stress conditions in soil environments, transgenic Arabidopsis lines had healthier growth status, higher green leaf percentage, lower malondialdehyde (MDA) content, and higher peroxidase (POD) activity than WT. The transcription factor ZmEREB180 may play a positive and promotional role in regulating the growth and development of maize root system and enhance the tolerance of maize plants under high salt, drought, osmosis, low nitrogen, and other adversity stresses. This study lays a good foundation for further identification of the biological function and molecular mechanism of transcription factor ZmEREB180 in maize.

Key words: maize (Zea mays L.), ZmEREB180, transcription factor, root system, abiotic stress, nitrogen use efficiency

Table 1

Primers used in this study"

引物名称Primer name 引物序列Primer sequence (5'-3')
pZmEREB180 OE-F CTGAAATCACCAGTCGGTACCATGTGCGGAGGCGCCATCC (Kpn I)
pZmEREB180 OE-R gcccttgctcaccatgGTACCTCAGAAAACAGAACCGTCG (Kpn I)
pZmEREB180RT-F CTGACGAGCTGGCGTTC
pZmEREB180RT-R TCAGAAAACAGAACCGTCG
pGAPDHRT-F CCCTTCATCACCACGGACTAC
pGAPDHRT-R AACCTTCTTGGCACCACCCT
pActinRT-F CCGTGAAGCCAGAAGCTACG
pActinRT-R AACTTGTGGCCGTTTACGTCG
pZmEREB180-F CTGACGAGCTGGCGTTC
pZmEREB180-R TCAGAAAACAGAACCGTCG

Fig. 1

Bioinformatics analysis of ZmEREB180 gene and its protein A: protein spatial structure domain analysis; B: protein transmembrane prediction C: protein tertiary structure prediction; D: promoter sequence analysis."

Fig. 2

Relative expression level of ZmEREB180 in different maize tissues Different lowercase letters indicate significant differences at the 0.05 probability level."

Fig. 3

Relative expression level of ZmEREB180 in different abiotic stress A–D: the relative expression level of ZmEREB180 after treatments with osmotic stress, dehydration, low temperature, and high salt; *: P < 0.05; **: P < 0.01."

Fig. 4

Relative expression level of ZmEREB180 in high nitrogen (HN) and low nitrogen (LN) treatments A-B: the relative expression level of ZmEREB180 after high nitrogen (HN) and low nitrogen (LN) treatments; *: P < 0.05; **: P < 0.01."

Fig. 5

Relative expression level of ZmEREB180 under different phytohormone treatments A-E: the relative expression level of ZmEREB180 after treatments with JA, ABA, SA, 2,4-D, and GA; *: P < 0.05; **: P < 0.01. JA: jasmonic acid; ABA: abscisic acid; SA: salicylic acid; 2,4-D: 2,4-dichlorophenoxyacetic acid; GA: gibberellins."

Fig. 6

Subcellular localisation of ZmEREB180 protein in Nicotiana benthamiana leaves GFP: green fluorescent protein map; Merged: overlay diagram; Bright field: bright field map; CK: nicotiana benthamiana leaf transfected with empty vector; ZmEREB180-EGFP: nicotiana benthamiana leaf transfected with the target gene vector; Bar: 20 μm."

Fig. 7

PCR detection and relative expression level of Arabidopsis thaliana (T3) A: PCR assay of transgenic Arabidopsis thaliana plants (T3); B: RT-qPCR detection of Arabidopsis thaliana plants (T3); M: DL2000 marker; N: negative control; W: water control; WT: wild-type Arabidopsis thaliana; L-1-L-6: T3 transgenic Arabidopsis thaliana lines; **: P < 0.01; ***: P < 0.001."

Fig. 8

Phenotypic analysis of WT and transgenic Arabidopsis thaliana lines under gradient NaCl concentrations A-D: growth of transgenic Arabidopsis thaliana plants and WT plants on 1/2 MS medium treated with 0, 0.10, 0.15, and 0.18 mol L- 1 NaCl, respectively. E: mean data on the length of primary roots of plants on 1/2 MS medium; WT: wild type; L-3, L-5, and L-6: ZmEREB180 transgenic Arabidopsis thaliana lines; Bar: 1.5 cm; *: P < 0.05; **: P < 0.01."

Fig. 9

Phenotypic analysis of WT and transgenic Arabidopsis thaliana lines under gradient mannitol concentrations A-D: growth of transgenic Arabidopsis thaliana plants and WT plants on 1/2 MS medium treated with 0, 0.15, 0.20, and 0.30 mol L-1 mannitol, respectively. E: mean data on the length of primary roots of plants on 1/2 MS medium. Abbreviations are the same as those given in Fig. 8; Bar: 1.5 cm; *: P < 0.05; **: P < 0.01."

Fig. 10

Phenotypic analysis of Arabidopsis thaliana lines under drought treatment in soil A: the phenotype of transgenic and WT Arabidopsis thaliana plants; B: the rate of green leaves under drought treatment; C: MDA content; D: POD activity; E: the rate of green leaves under re-water treatment. Abbreviations are the same as those given in Fig. 8; *: P < 0.05; **: P < 0.01."

Fig. 11

Phenotypic analysis of transgenic lines and WT plants of Arabidopsis thaliana under high salt treatment in soil A: phenotype of transgenic and WT Arabidopsis thaliana plants; B: the rate of green leaves under high salt treatment; C: MDA content; D: POD activity. Abbreviations are the same as those given in Fig. 8; *: P < 0.05; **: P < 0.01."

[1] 戴景瑞, 鄂立柱. 百年玉米,再铸辉煌——中国玉米产业百年回顾与展望. 农学学报, 2018, 8(1): 83-88.
doi: 10.11923/j.issn.2095-4050.cjas2018-1-083
Dai J R, E L Z. From the past centennial progress to more brilliant achievements in the future: the history and prospects of maize industrialization in China. J Agric, 2018, 8(1): 83-88 (in Chinese with English abstract).
[2] 仇焕广, 李新海, 余嘉玲. 中国玉米产业:发展趋势与政策建议. 农业经济问题, 2021, 42(7): 4-16.
Qiu H G, Li X H, Yu J L. China maize industry: development trends and policy suggestions. Issue Agric Econ, 2021, 42(7): 4-16 (in Chinese with English abstract).
[3] 张静. 逆境胁迫下玉米DNA去甲基化酶和AP2/ERF基因家族的全基因组鉴定与分析. 安徽师范大学硕士学位论文, 安徽芜湖, 2020.
Zhang J. Genome-wide Identification and Analysis of Maize DNA Demethylase and AP2/ERF Gene Family under Abiotic Stress. MS Thesis of Anhui Normal University, Wuhu, Anhui, China, 2020 (in Chinese with English abstract).
[4] Baillo E H, Kimotho R N, Zhang Z B, Xu P. Transcription factors associated with abiotic and biotic stress tolerance and their potential for crops improvement. Genes, 2019, 10: 771.
[5] Allen M D, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M. A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J, 1998, 17: 5484-5496.
doi: 10.1093/emboj/17.18.5484 pmid: 9736626
[6] 张麒, 陈静, 李俐, 赵明珠, 张美萍, 王义. 植物AP2/ERF转录因子家族的研究进展. 生物技术通报, 2018, 34(8): 1-7.
doi: 10.13560/j.cnki.biotech.bull.1985.2017-1142
Zhang Q, Chen J, Li L, Zhao M Z, Zhang M P, Wang Y. Research progress on plant AP2/ERF transcription factor family. Biotechnol Bull, 2018, 34(8): 1-7 (in Chinese with English abstract).
doi: 10.13560/j.cnki.biotech.bull.1985.2017-1142
[7] Zhu Y Q, Liu Y, Zhou K M, Tian C Y, Aslam M, Zhang B L, Liu W J, Zou H W. Overexpression of ZmEREBP60 enhances drought tolerance in maize. J Plant Physiol, 2022, 275: 153763.
[8] Fu J Y, Zhu C Y, Wang C, Liu L J, Shen Q Q, Xu D B, Wang Q. Maize transcription factor ZmEREB20 enhanced salt tolerance in transgenic Arabidopsis. Plant Physiol Bioch, 2021, 159: 257-267.
[9] Yu Y, Yu M, Zhang S X, Song T Q, Zhang M, Zhou H W, Wang Y K, Xiang J S, Zhang X K. Transcriptomic identification of wheat AP2/ERF transcription factors and functional characterization of TaERF-6-3A in response to drought and salinity stresses. Int J Mol Sci, 2022, 23: 3272.
[10] 艾蓉, 张春, 悦曼芳, 邹华文, 吴忠义. 玉米转录因子ZmEREB211对非生物逆境胁迫的应答. 作物学报, 2023, 49: 2433-2445.
doi: 10.3724/SP.J.1006.2023.23071
Ai R, Zhang C, Yue M F, Zou H W, Wu Z Y. Response of maize transcriptional factor ZmEREB211 to abiotic stress. Acta Agron Sin, 2023, 49: 2433-2445 (in Chinese with English abstract).
[11] Chen K, Tang W S, Zhou Y B, Chen J, Xu Z S, Ma R, Dong Y S, Ma Y Z, Chen M. AP2/ERF transcription factor GmDREB1 confers drought tolerance in transgenic soybean by interacting with GmERFs. Plant Physiol Bioch, 2022: 170: 287-295.
[12] Oliveira P N, Matias F, Martinez-Andujar C, Martinez-Melgarejo P A, Prudencio A S, Galeano E, Perez-Alfocea F, Carrer H. Overexpression of TgERF1, a transcription factor from tectona grandis, increases tolerance to drought and salt stress in tobacco. Int J Mol Sci, 2023, 24: 4149
[13] Cai X X, Chen Y, Wang Y, Shen Y, Yang J K, Jia B W, Sun X L, Sun M Z. A comprehensive investigation of the regulatory roles of OsERF096, an AP2/ERF transcription factor, in rice cold stress response. Plant Cell Rep, 2023, 42: 2011-2022.
[14] Seok H Y, Tran H T, Lee S Y, Moon Y H. AtERF71/HRE2, an Arabidopsis AP2/ERF transcription factor gene, contains both positive and negative cis-regulatory elements in its promoter region involved in hypoxia and salt stress responses. Int J Mol Sci, 2022, 23: 5310.
[15] Yu F, Liang K, Fang T, Zhao H, Han X, Cai M, Qiu F. A group VII ethylene response factor gene, ZmEREB180, coordinates waterlogging tolerance in maize seedlings. Plant Biotechnol J, 2019, 17: 2286-2298.
[16] Xiao Y Y, Chen J Y, Kuang J F, Shan W, Xie H, Jiang Y M, Lu W J. Banana ethylene response factors are involved in fruit ripening through their interactions with ethylene biosynthesis genes. J Exp Bot, 2013, 64: 2499-2510.
[17] Shi Q, Dong Y B, Zhou Q, Qiao D H, Ma Z Y, Zhang L, Li Y L. Characterization of a maize ERF gene, ZmERF1, in hormone and stress responses. Acta Physiol Plant, 2016, 38: 1-9.
[18] El-Sharkawy I, Kim W S, El-Kereamy A, Jayasankar S, Svircev A M, Brown D C. Isolation and characterization of four ethylene signal transduction elements in plums (Prunus salicina L.). J Exp Bot, 2007, 58: 3631-3643.
pmid: 18057041
[19] Zang Z L, Lv Y, Liu S, Yang W, Ci J B, Ren X J, Wang Z, Wu H, Ma W Y, Jiang L Y, Yang W G. A Novel ERF Transcription factor, ZmERF105, positively regulates maize resistance to Exserohilum turcicum. Front Plant Sci, 2020, 11: 850.
[20] Huang Y, Zhang B L, Sun S, Xing G M, Wang F, Li M Y, Tian Y S, Xiong A S. AP2/ERF Transcription factors involved in response to tomato yellow leaf curly virus in tomato. Plant Genome, 2016, 9: 1-15.
[21] Liu J X, Li J Y, Wang H N, Fu Z D, Liu J, Yu Y X. Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments. J Exp Bot, 2011, 62: 825-840.
doi: 10.1093/jxb/erq324 pmid: 20974735
[22] Zang Z Y, Wang Z, Zhao F X, Yang W, Ci J B, Ren X J, Jiang L Y, Yang W G. Maize ethylene response factor ZmERF061 is required for resistance to Exserohilum turcicum. Front Plant Sci, 2021, 12: 630413.
[23] Gao T, Li G Z, Wang C R, Dong J, Yuan S S, Wang Y H, Kang G Z. Function of the ERFL1a transcription factor in wheat responses to water deficiency. Int J Mol Sci, 2018, 19: 1465.
[24] Liu D F, Chen X J, Liu J Q, Ye J C, Guo Z J. The rice ERF transcription factor OsERF922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance. J Exp Bot, 2012, 63: 3899-3911.
[25] Zhu J T, Wei X N, Yin C S, Zhou H, Yan J H, He W X, Yan J B, Li H. ZmEREB57 regulates OPDA synthesis and enhances salt stress tolerance through two distinct signalling pathways in Zea mays. Plant Cell Environ, 2023, 46: 2867-2883.
[26] Hu Z Y, Wang X J, Wei L, Wansee S, Rabbani N H, Chen L, Kang Z S, Wang J F. TaAP2-10, an AP2/ERF transcription factor, contributes to wheat resistance against stripe rust. J Plant Physiol, 2023, 288: 154078.
[27] Li H, Xiao Q L, Zhang C X, Du J, Li X, Huang H H, Wei B, Li Y P, Yu G W, Liu H M, Hu Y F, Liu Y H, Zhang J J, Huang Y B. Identification and characterization of transcription factor ZmEREB94 involved in starch synthesis in maize. J Plant Physiol, 2017, 216: 11-16.
[28] Zhang Y, Ji A J, Xu Z C, Luo H M, Song J Y. The AP2/ERF transcription factor SmERF128 positively regulates diterpenoid biosynthesis in Salvia miltiorrhiza. Plant Mol Biol, 2019, 100: 83-93.
doi: 10.1007/s11103-019-00845-7 pmid: 30847712
[29] Wang M Y, Gao M, Zhao Y X, Chen Y C, Wu L W, Yin H F, Yang J H, Xiong S F, Wang S Q, Wang J, Yang Y, Wang J, Wang Y D. LcERF19, an AP2/ERF transcription factor from Litsea cubeba, positively regulates geranial and neral biosynthesis. Hortic Res, 2022, 9: uhac093.
[30] Wei S B, Li X, Lu Z F, Zhang H, Ye X Y, Zhou Y J, Li J, Yan Y Y, Pei H C, Duan F Y, Wang D Y, Chen S, Wang P, Zhang C, Shang L G, Zhou Y, Yan P, Zhao M, Huang J R, Bock R, Qian Q, Zhou W B. A transcriptional regulator that boosts grain yields and shortens the growth duration of rice. Science, 2022, 377: eabi8455.
[31] Srivastava R, Kumar R. The expanding roles of APETALA2/ Ethylene Responsive Factors and their potential applications in crop improvement. Brief Funct Genomics, 2019, 18: 240-254.
doi: 10.1093/bfgp/elz001
[32] Zhang C, Li X L, Wang Z P, Zhang Z B, Wu Z Y. Identifying key regulatory genes of maize root growth and development by RNA sequencing. Genomics, 2020, 112: 5157-5169.
doi: 10.1016/j.ygeno.2020.09.030 pmid: 32961281
[33] 高建强, 梁华, 赵军. 植物遗传转化农杆菌浸花法研究进展. 中国农学通报, 2010, 26(16): 22-25.
Gao J Q, Liang H, Zhao J. Progress on the floral-dip method of Agribacterium-mediated plant transformation. Chin Agric Sci Bull, 2010, 26(16): 22-25 (in Chinese with English abstract).
[34] 悦曼芳, 张春, 郑登俞, 邹华文, 吴忠义. 玉米转录因子ZmbHLH91对非生物逆境胁迫的应答. 作物学报, 2022, 48: 3004-3017.
doi: 10.3724/SP.J.1006.2022.13060
Yue M F, Zhang C, Zheng D Y, Zou H W, Wu Z Y. Response of maize transcriptional factor ZmbHLH91 to abiotic stress. Acta Agron Sin, 2022, 48: 3004-3017 (in Chinese with English abstract).
[35] Zhang H M, Zhu J H, Gong Z Z, Zhu J K. Abiotic stress responses in plants. Nat Rev Genet, 2022, 23: 104-119.
[36] 王畅. 玉米转录因子ZmEREB20盐胁下的响应及调控机制初步分析. 四川农业大学硕士学位论文, 四川雅安, 2019.
Wang C. Functional Identification of Maize Transcription Factor ZmEREB20 under Salt Stress. MS Thesis of Sichuan Agricultural University, Ya’an, Sichuan, China, 2019 (in Chinese with English abstract).
[1] Chen Guo-Huan, Zhang Rui, Li Yan-Di, Zhao Jia-Qi, Ren Yong-Tao, Zhang Tian-Ci, Guo Hua-Chun, Li Jun, Yang Fang. Effects of foliar application of exogenous selenium on anthocyanin biosynthesis in tubers of light purple-fleshed potatoes [J]. Acta Agronomica Sinica, 2026, 52(6): 1876-1890.
[2] Wang Wen-Yuan, Yan Xue-Jia, Liu Yu-Lin, Sun Xiao-Tong, Li Ya-Nan, Tang Xin-Hua, Shi Ying. Screening of low-light-tolerant potato varieties and cloning and functional analysis of the transcription factor gene StPIF3 [J]. Acta Agronomica Sinica, 2026, 52(6): 1631-1645.
[3] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[4] Cai Zhao-Qin, He Guan-Yong, He Wen, Ruan Li-Xia, Liang Zhen-Hua, Li Yong-Zhen, Li Heng-Rui, Chen Hui-Xian. Dynamic transcriptome analysis and key gene discovery during cassava branching development [J]. Acta Agronomica Sinica, 2026, 52(5): 1430-1441.
[5] Gu Chun-Miao, Wang Run-Feng, Huang Lu, Liu Hao, Lu Qing, Li Hai-Fen, Li Shao-Xiong, He Shuang-Cheng, Hong Yan-Bin, Chen Xiao-Ping, Tan Bin, Yu Qian-Xia. Genome-wide analysis of the WOX gene family and identification of candidate genes for adventitious shoot regeneration in peanut [J]. Acta Agronomica Sinica, 2026, 52(5): 1326-1340.
[6] Wang Yi-Han, Li Fu-Chang, Liu Yi, Zhu Guo-Peng. Cloning of the IbOPR2 gene promoter and identification of regulatory factors in sweetpotato [J]. Acta Agronomica Sinica, 2026, 52(4): 1268-1276.
[7] Yang Ying, Hao Yu-Wan, Zhang Xue-Ning, Fang Jia-Lu, Ma Yue-Hua, Yang Wei-Long, Sun Wen-Qing, Wang Xin-Chao, Wang Yu-Chun, Huang Jian-Yan. Molecular mechanism of CsERF9-mediated regulation of anthracnose resistance in tea plant [J]. Acta Agronomica Sinica, 2026, 52(4): 1103-1115.
[8] Yang Biao, Du Shuai-Kang, Zhang Ji-Wang, Shi Ying, Zhang Li-Li. Genome-wide identification of class III POD gene family in potato and its expression profile analysis [J]. Acta Agronomica Sinica, 2026, 52(2): 405-420.
[9] Jing Xiu-Qing, Cai Yong-Duo, Deng Ning, Zhao Xiao-Dong, Zhai Fei-Hong, Zeng Qun. Identification and expression pattern analysis of RopGEF family genes in Chenopodium quinoa [J]. Acta Agronomica Sinica, 2026, 52(1): 28-43.
[10] MA Juan-E, YAO You-Hua, YAO Xiao-Hua, WU Kun-Lun, CUI Yong-Mei. Cloning and functional analysis of HvERF039 gene in Qingke (Hordeum vulgare L. var. nudum Hook. f) [J]. Acta Agronomica Sinica, 2025, 51(9): 2341-2357.
[11] YAN Zhe-Lin, REN Qiang, FAN Zhi-Long, YIN Wen, SUN Ya-Li, FAN Hong, HE Wei, HU Fa-Long, YAN Li-Juan, CHAI Qiang. Postponed nitrogen application optimizes interspecific interactions and enhances nitrogen use efficiency in wheat-maize intercropping systems in an oasis irrigation region [J]. Acta Agronomica Sinica, 2025, 51(8): 2190-2203.
[12] YANG Hai-Yang, WU Lin-Xuan, LI Bo-Wen, SHI Han-Feng, YUAN Xi-Long, LIU Jin-Zhao, CAI Hai-Rong, CHEN Shi-Yi, GUO Tao, WANG Hui. OsWRI3, identified based on QTL mapping, regulates seed shattering in rice [J]. Acta Agronomica Sinica, 2025, 51(7): 1712-1724.
[13] YAN Zhi-Lan, ZHAO Qin, CHANG Tian-Da, WANG Yi-Ming, WANG Bi-Hui, WANG Peng, HUANG Chun-Guo, ZHANG Hui, WANG Li-Xiang, HAO Xiao-Peng, ZHAO Bo. Genome-wide identification and characterization of Alternative oxidase (AOX) genes in leguminous crops and their expression patterns in response to abiotic stresses in common bean [J]. Acta Agronomica Sinica, 2025, 51(7): 1769-1783.
[14] FANG Ying-Hao, ZHOU Bo, CHEN Ru-Mei, YANG Wen-Zhu, QIN Hui-Min. Integrative analysis of RNA-seq and PER-seq to elucidate regulatory network of ZmHDZ6 expression [J]. Acta Agronomica Sinica, 2025, 51(4): 958-968.
[15] PAN Ju-Zhong, WEI Ping, ZHU De-Ping, SHAO Sheng-Xue, CHEN Shan-Shan, WEI Ya-Qian, GAO Wei-Wei. Cloning and functional analysis of OsERF104 transcription factor in rice [J]. Acta Agronomica Sinica, 2025, 51(4): 900-913.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd