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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (01): 17-24.doi: 10.3724/SP.J.1006.2008.00017

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Evaluation and Validation of Four Molecular Markers Associated with Pre-Harvest Sprouting Tolerance in Chinese Wheats

YANG Yan12,ZHAO Xian-Lin2, ZHANG Yong2,CHEN Xin-Min2,HE Zhong-Hu2,YU Zhuo1,XIA Lan-Qin2*
  

  1. 1 College of Biology Engineering, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia; 2 National Wheat Improvement Center, Institute of Crop Sciences / The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China

  • Received:2007-06-13 Revised:1900-01-01 Online:2008-01-12 Published:2008-01-12
  • Contact: XIA Lan-Qin

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Abstract:

Pre-harvest sprouting (PHS) of wheat (Triticum aestivum L.) reduces the quality and economic value of the grain. Increasing PHS tolerance is one of the most important objectives in wheat breeding. In this study, three PHS tolerance- associated markers MST101, wmc104, and Xgwm155, as well as Vp1B3, which was developed in our previous study, were employed to evaluate 95 Chinese landraces and historical varieties, aimed at assessing the efficiency of these markers in selecting genotypes with higher PHS tolerance. Among the 95 genotypes tested, 57, 19, and 19 accessions had germination indexes (GI) less than 30%, 30%–60%, and over 60%, respectively. The relationships between the variation of the PCR products of the four markers and the germination indexes of the tested accessions were analyzed. Variations at Vp1B3 and Xgwm155 were associated with PHS resistance, but not the STS marker MST101 and STMS marker wmc104. Whereas Vp1B3 was more effective than Xgwm155 for selecting PHS resistant varieties, the selection efficiency would be greatly improved by use of both markers.

Key words:

Bread wheat, Pre-harvest sprouting, Molecular marker, Vp1B3, Xgwm155, MST101, wmc104

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