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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1146-1150.doi: 10.3724/SP.J.1006.2009.01146

• RESEARCH NOTES • Previous Articles     Next Articles

Drought and Salinity Tolerance in Transgenic Potato Expressing the Betaine Aldehyde Dehydrogenase Gene

ZHANG Ning12,SI Huai-Jun12,LI Liang1,YANG Tao12,ZHANG Chun-Feng1,WANG Di1*   

  1. 1Gansu Key Laboratory of Crop Genetic & Germplasm enhancement,Gansu Agricultural University,Lanzhou 730070,China;2College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2008-10-29 Revised:2009-02-13 Online:2009-06-12 Published:2009-04-16
  • Contact: WANG Di,E-mail:wangd@gsau.edu.cn

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Abstract:

Glycine betaine (GB) is a common compatible solute in many different organisms including higher plants. Many plant species can accumulate GB in response to drought and salinity. GB is synthesized by conversion of choline to GB through a two-step oxidation via the intermadiate betaine aldehyde. In higher plants, the relevant enzymes are choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). The fact that many important crops, such as rice, potato and tomato, are betaine-deficient has inevitably led to the proposal that it might be possible to increase drought and salinity tolerances by genetic engineering of GB synthesis. In the present study, the transgenic plants of potato cultivar Gannongshu 2 were obtained by Agrobacterium-mediated transformation of the expression vector pBIBB contained BADH gene under the control of the constitutive promoter CaMV 35S. PCR, Southern and Northern blot analyses showed that the BADH gene was integrated into potato genome, transcribed and expressed in the transgenic plants. The analysis of BADH activity of transgenic plant leaves revealed that the BADH activity ranged from 2 to 11 U, while it was not detectable in the control plants. There was a negative relationship (y= –3.7738x+57.083, r=0.989**) between BADH activity and relative electric conductivity of the transgenic potato leaves. The transgenic potato plants grew normally under NaCl and polyethylene glycol (PEG) stresses with increase of 0.4–0.9 cm for plant height and 17–29% for fresh weight per plant compared with the control plants. This result demonstrated that the transgenic potato plants can improve tolerances to drought and salinity as a result of transformation and expression of BADH gene.

Key words: Potato, Betaine aldehyde dehydrogenase, Genetic transformation, Drought resistance, Salt tolerance

[1] Deblonde P M K, Ledent J F. Effects of moderate drought condition on green leaf number, stem height, leaf length and tuber yield of potato cultivars. Eur J Agron, 2001, 14: 31-41
[2] Chen T H H, Murata N. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol, 2002, 5: 250-257

[3] Rathinasabapathi B, McCue K F, Gage D A, Hanson A D. Metabolic engineering of glycine betaine synthesis: Plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. Planta, 1994, 193: 155-162

[4] Ishitani M, Nakamura T, Han S Y, Takabe T. Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid. Plant Mol Biol, 1995, 27: 307-315

[5] Guo Y(郭岩), Zhang L(张莉), Xiao G(肖岗), Cao S-Y(曹守云), Gu D-M(谷冬梅), Tian W-Z(田文忠), Chen S-Y(陈受宜). Expression of betaine aldehyde dehydrogenase gene and salinity tolerance in rice transgenic plants. Sci China(Ser.C)(中国科学·C辑), 1997, 27(2): 151-155 (in Chinese)

[6] Kishitani S, Takanami T, Suzuki M, Oikawa M, Yokoi S, Ishitani M, Alvarez-Nakase A M, Takabe T, Takabe T. Compatibility of glycinebetaine in rice plants: Evaluation using transgenic rice plants with a gene for peroxisomal betaine aldehyde dehydrogenase from barley. Plant Cell Environ, 2000, 23: 107-114

[7] Guo B-H(郭北海), Zhang Y-M(张艳敏), Li H-J(李洪杰), Du L-C(杜立群), Li Y-X(李银心), Zhang J-S(张劲松), Chen S-Y(陈受宜), Zhu Z-Q(朱至清). Transformation of wheat with a gene encoding for the betaine aldehyde dehydrogenase (BADH). Acta Bot Sin (植物学报), 2000, 42(3): 279-283 (in Chinese with English abstract)

[8] Li Y-X(李银心), Chang F-Q(常凤启), Du L-Q(杜立群), Guo B-H(郭北海), Li H-J(李洪杰), Zhang J-S(张劲松), Chen S-Y(陈受宜), Zhu Z-Q(朱至清). Genetic transformation of watercress with a gene encoding for betaine-aldehyde dehydrogenase (BADH). Acta Bot Sin (植物学报), 2000, 42(5): 480-484 (in Chinese with English abstract)

[9] Liu F-H(刘凤华), Guo Y(郭岩), Gu D-M(谷冬梅), Xiao G(肖岗), Chen Z-H(陈正华), Chen S-Y(陈受宜). Salt tolerance of transgenic plants with BADH cDNA. Acta Genet Sin (遗传学报), 1997, 24(1): 54-58 (in Chinese with English abstract)

[10] Chen C-F(陈传芳), Li Y-W(李义文), Chen Y(陈豫), Bai J-R(白建荣), Li H(李辉), Zhu Y-F(朱银锋), Chen S-Y(陈受宜), Jia X(贾旭). Saline tolerance white clover transformed with the betaine aldehyde dehyrogenase gene by Agrobacterium tumefaciens. Acta Genet Sin (遗传学报), 2004, 31(1): 97-101 (in Chinese with English abstract)

[11] Luo X-L(罗晓丽), Xiao J-L(肖娟丽), Wang Z-A(王志安), Zhang A-H(张安红), Tian Y-C(田颖川), Wu J-H(吴家和). Overexpression of Spinacia oleracea betaine aldehyde dehydrogenase (SoBADH) gene confers the salt and cold tolerant in Gossypium hirsutum L. Chin J Biotechnol (生物工程学报), 2008, 24(8): 1464-1469 (in Chinese with English abstract)

[12] Zhang N(张宁), Wang D(王蒂), Si H-J(司怀军). Isolation and induced expression of betaine aldehyde dehydrogenase gene from spinach. J Agric Biotechnol (农业生物技术学报), 2004, 12(5): 612-613 (in Chinese)

[13] Si H-J(司怀军), Zhang N(张宁), Wang D(王蒂). Enhancement of drought and salt resistances in tobacco by transformation of betaine aldehyde dehydrogenase Gene. Acta Agron Sin (作物学报), 2007, 33(8): 1335-1339 (in Chinese with English abstract)

[14] Liu J(柳俊), Xie C-H(谢从华), Huang D-E(黄大恩), Liao Y(廖勇), Wu C-J(吴承金). Research on forming mechanism of potato microtubers-effects of BA on the formation and growth of microtubers. Chin Potato J (马铃薯杂志), 1995, 9(1): 7-11(in Chinese with English abstract)

[15] Si H-J(司怀军), Xie C-H(谢从华), Liu J(柳俊). An efficient protocol for Agrobacterium-mediated transformation of microtuber and the introduction of an antisense class I patatin gene into potato. Acta Agron Sin (作物学报), 2003, 29(6): 801-805(in English with Chinese abstract)

[16] Edwards K, Johnstone C, Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl Acids Res, 1991, 19: 1349

[17] Chomczynski P, Sacchi N. Single step method of RNA isolation by acid guainidium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987, 162: 156-159

[18] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anayti Biochem, 1976, 72: 248-254

[19] Li H-S(李合生). Principles and Techniques of Plant Physiological and Biochemical Experiment (植物生理生化实验原理与技术). Beijing: Higher Education Press, 2000. pp 261-263(in Chinese)

[20] McCue K F, Hanson A D. Drought and salt tolerance: towards understanding and application. Trends Biotechnol, 1990, 8: 358-362

[21] Yancey P H, Clark M E, Hand S C, Bowlus R D, Somero G N. Living with water stress: Evolution of osmolyte systems. Science, 1982, 217: 1214-1222
Weigle P, Weretilnyk E A, Hanson A D. Betaine aldehyde oxidation by spinach chloroplasts. Plant Physiol, 1986, 82: 753-759
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