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Acta Agronomica Sinica ›› 2009, Vol. 35 ›› Issue (7): 1217-1228.doi: 10.3724/SP.J.1006.2009.01217

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Associated Analysis between Temporal and Spatial Expression of Fiber Development Genes and Fiber Quality

JU Ming, WANG Hai-Tang, WANG Li-Ke, LI Fei-Fei, WU Shen-Jie, ZHU Hua-Yu, ZHANG T   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2008-10-10 Revised:2009-03-20 Online:2009-07-12 Published:2009-05-19
  • Contact: GUO Wang-Zhen, E-mail: moelab@njau.edu.cn

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Abstract:

Ten genes expressed preferentially in fiber development period reported previously and 14 cotton cultivars (strains) with distinctly different fiber quality were selected in this paper. To test the relative expression values of the genes in six different fiber developmental stages, 0 day post anthesis (DPA), 5 DPA, 10 DPA, 15 DPA, 20 DPA, 23 DPA, by real-time quantitative RT-PCR (qRT-PCR), and the data of fiber qualities from 14 cotton varieties (strains). The expression profile showed that GhExp1, GhCIPK1,GhSus1, GhSusA1 and GhPL genes were expressed preferentially during fiber elongation; GhACT1、GhRacA and GhRacB genes all had high expression level in earlier stage of fiber elongation and the thickening period of secondary cell wall. Two cellulose synthase genes (GhCelA1 and GhCelA3) were expressed predominantly during late stage of fiber elongation and the thickening period of secondary cell wall. For most genes, the expression value in low expression level period had significant correlation with fiber quality, while no significant correlation was detected in preferential expression stage of these genes with an exception of GhRacA gene. The expression level of GhExp1 in 20DPA fiber of 14 cotton varieties (strains) had a significant negative correlation with fiber strength and uniformity and a significant positive correlation with fiber elongation percentage; the expression level of GhPL gene in 23DPA had a significant negative correlation with fiber length; the expression level of GhRacA gene in 5DPA and 23DPA both had a high significant positive correlation with fiber elongation percentage; the expression level of GhRacB gene in 10DPA had a significant negative correlation with fiber length and uniformity; the expression level of GhCelA1 gene in 5DPA had a significant positive correlation with fiber length and a significant negative correlation with micronaire value; the expression level of GhCelA1 gene in 10DPA had a significant negative correlation with fiber length, a significant positive correlation with micronaire value, and a high significant correlation with fiber elongation percentage; the correlation between the expression levels of GhCIPK1, GhACT1, GhSus1, GhSusA1and GhCelA3 gene and fiber quality indexes had not been detected.

Key words: Cotton, Fiber quality, Genes related with fiber development, Real-time quantitative RT-PCR

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