大豆品种豫豆25抗疫霉根腐病基因的鉴定

doi:10.3724/SP.J.1006.2009.01844

Abstract

Abstract:

Phytophthora root rot, caused by Phytophthora sojae, is a destructive disease on soybean. Use of resistant soybean cultivars is the most economical and effective method for controlling the disease. Up to now, nine loci for the resistance with 15 genes have been identified in soybean. However, only a few genes, such as Rps1c and Rps1k,were effectively resistant to populations of P. sojae in China, so mining new resistance genes is necessary greatly for the disease control. Soybean cv. Yudou 25 has broad spectrum resistance to P. sojae, and is an elite resistance source for Phytophthora root rot of soybean. To effectively utilize the cultivar in resistance breeding, in the present study, we identified and tagged the Phytophthora resistance gene in the cultivar by using SSR markers and bulked segregation analysis (BSA). Two F_2:3populations were developed for resistance genetic analysis and resistance gene mapping. Using hypocotyls inoculation technique at the seedling stage in the glasshouse, the reaction to P. sojae isolate PSMC1 (virulence type 1b, 1d, 3a, 3b, 3c, 4, 5, 6, 7) in 82 and 98 F_2:3families derived from two crosses of Yudou 21×Yudou 25 and Zaoshu 18×Yudou 25, respectively, were identified. The segregation ratio in both populations fit into 1:2:1 for homozygous resistant, segregating and homozygous susceptible, showing that the cultivar resistance to Phytophthora root rot is controlled by a dominant single gene, with the temporary name of RpsYD25. On the basis of linkage analysis with SSR markers, RpsYD25 was located on soybean molecular linkage group (MLG) N in both populations. Five SSR markers were associated with RpsYD25 in an order of Sat_208-Satt530-RpsYD25-Sat_084-Satt125-Sat_236 in F_2:3population from the cross of Yudou 21×Yudou 25, RpsYD25 was flanked by Satt530 and Sat_084 with a distance of 6.3 and 7.7 cM, respectively. Five SSR markers were linked to RpsYD25 in an order of Satt125-RpsYD25-Sat_275-Sat_266-Satt660-GMABAB in F_2:3population from the cross of Zaoshu 18×Yudou 25, RpsYD25 was flanked by Satt125 and Sat_275 with a distance of 7.9 and 7.8 cM, respectively. Because RpsYD25 was mapped on MLG N near to Rps1 locus, the genetic relationship of RpsYD25 and Rps1 was detected by using the selected SSR markers contained in the Rps1k allele sequence. A SSR marker Rps1k6 in Rps1k allele was found to be linked to RpsYD25 with a genetic distance of 19.4 cM in F_2:3population from the crosses Yudou 21×Yudou 25. Therefore, the Phytophthora resistance gene RpsYD25 in cv. Yudou 25 might be a novel allele at Rps1 locus, or a novel gene.

Key words: Glycine max, Phytophthora root rot, Phytophthora sojae, Resistance gene, SSR marker

FAN Ai-Ying,WANG Xiao-Ming,FANG Xiao-Ping,WU Xiao-Fei,ZHU Zhen-Dong. Molecular Identification of Phytophthora Resistance Gene in Soybean Cultivar Yudou25[J].Acta Agron Sin, 2009, 35(10): 1844-1850.

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Molecular Identification of Phytophthora Resistance Gene in Soybean Cultivar Yudou25

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