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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (12): 2994-3003.doi: 10.3724/SP.J.1006.2022.13076

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of SSR-DNA fingerprints and genetic diversity analysis of 290 maize varieties approved in Jilin province, China

TIAN Hong-Li1(), ZHAO Zi-Wei1,2(), YANG Yang1(), FAN Ya-Ming1, BAN Xiu-Li3, YI Hong-Mei1, YANG Hong-Ming3, LIU Shao-Rong1, GAO Yu-Qian3, LIU Ya-Wei1, WANG Feng-Ge1()   

  1. 1Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences / Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097, China
    2Jilin Agricultural University, Changchun 130118, Jilin, China
    3Jilin Seed Management Station, Changchun 130022, Jilin, China
  • Received:2021-12-21 Accepted:2022-03-25 Online:2022-12-12 Published:2022-04-20
  • Contact: WANG Feng-Ge E-mail:tianhongli9963@163.com;zhao691111@sina.com;caurwx@163.com;gege0106@163.com
  • About author:First author contact:

    **Contributed equally to this work

  • Supported by:
    Science and Technology Innovation Capacity Building Project of Beijing Academy of Agriculture and Forestry Sciences(KJCX20200305);National Key Research and Development Program of China during 13th Five-Year Plan(2017YFD0102001)

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Abstract:

The standard fingerprint construction and genetic diversity analysis of maize varieties approved in Jilin province can provide data support for variety approval, protection, and market monitoring. It is also of great significance for variety breeding and germplasm innovation. In this study, 40 pairs of SSR markers were used to construct the standard SSR-DNA fingerprints of 290 maize varieties approved in Jilin province from 2010 to 2017, and the genetic diversity was analyzed. The results showed that the average number of alleles detected by 40 SSRs was 11.85 and the average PIC was 0.64. The average frequency of heterozygous genotypes of varieties was 0.66. The different loci number among varieties was ≥ 2. The genetic diversity analysis of six maturity groups revealed that there were more varieties with middle and late maturity, accounting for 71.72%, and fewer varieties with extremely early maturity, early maturity, and late maturity. The early maturity group had the highest gene diversity parameter of 0.69, and the mid-late maturity group with the largest number of varieties had a relatively low gene diversity value. Clustering results indicated that the varieties approved and popularized in Jilin province were divided into three categories: sweet waxy and popcorn, extremely early and early middle, medium or late maturity. The middle or late maturing varieties had two major trends, representing the differences in the selection of heterozygous groups, the different breeding units, the differences in variety types and so on. This study provides an important theoretical and data basis for maize variety management, variety breeding, and germplasm innovation in Jilin province.

Key words: Jilin province, approved maize varieties, SSR marker, DNA fingerprints construction, genetic diversity

Table 1

Polymorphism information of 40 SSR primers"

编号
SSR ID		 染色体位置
Chromosome location		 基因型数
Number of
genotypes		 等位基因数
Number of
alleles		 多态信息含量
Polymorphism
information content		 引物区分能力
Discrimination power		 主要等位基因及其频率
Major allele and its
frequency
P01 1.03 46.00 18.00 0.73 0.92 350,0.44
P02 1.06 16.00 8.00 0.52 0.64 241,0.62
P03 2.04 66.00 21.00 0.81 0.92 256,0.28
P04 2.08 66.00 25.00 0.79 0.92 358,0.37
P05 3.00 69.00 23.00 0.81 0.95 291,0.36
P06 3.05 19.00 8.00 0.68 0.86 336,0.41
P07 4.01 28.00 14.00 0.38 0.53 411,0.77
P08 4.06 35.00 18.00 0.67 0.84 382,0.39
P09 5.03 56.00 19.00 0.80 0.93 319,0.32
P10 5.07 42.00 15.00 0.81 0.93 252,0.27
P11 6.00 69.00 22.00 0.84 0.95 183,0.26
P12 6.05 47.00 20.00 0.64 0.86 265,0.57
P13 7.00 33.00 14.00 0.71 0.87 208,0.45
P14 7.04 16.00 6.00 0.66 0.85 173,0.46
P15 8.06 32.00 8.00 0.71 0.89 237,0.46
P16 8.08 21.00 8.00 0.51 0.75 217,0.65
P17 9.03 9.00 4.00 0.56 0.77 413,0.48
P18 9.04 9.00 6.00 0.33 0.57 278,0.76
P19 10.02 22.00 14.00 0.49 0.73 222,0.66
P20 10.05 25.00 10.00 0.68 0.86 185,0.42
P21 1.07 7.00 4.00 0.43 0.66 154,0.67
P22 1.10 83.00 24.00 0.85 0.95 193,0.31
P23 2.00 26.00 8.00 0.68 0.84 253,0.44
P24 2.07 23.00 8.00 0.61 0.82 222,0.51
P25 2.09 35.00 13.00 0.68 0.87 165,0.47
P26 3.07 13.00 6.00 0.56 0.77 232,0.56
P27 4.04 20.00 8.00 0.64 0.83 271,0.44
P28 4.09 9.00 5.00 0.51 0.74 197,0.48
P29 5.02 40.00 12.00 0.79 0.92 276,0.28
P30 5.03 8.00 5.00 0.45 0.63 126,0.61
P31 6.01 52.00 20.00 0.75 0.91 263,0.39
P32 6.07 24.00 9.00 0.62 0.80 234,0.54
P33 7.01 24.00 9.00 0.66 0.81 207,0.39
P34 7.03 16.00 8.00 0.50 0.73 170,0.60
P35 8.02 20.00 8.00 0.69 0.88 183,0.44
P36 8.09 16.00 6.00 0.62 0.83 204,0.50
P37 9.01 27.00 8.00 0.76 0.90 197,0.29
P38 9.05 7.00 4.00 0.37 0.65 275,0.61
P39 10.00 34.00 13.00 0.64 0.83 309,0.44
P40 10.04 41.00 15.00 0.71 0.86 310,0.37
平均Mean — 31.28 11.85 0.64 0.82 0.47

Fig. 1

Distribution of heterozygous genotype rate and different locus number obtained by pairwise comparative analysis in 290 maize hybrid lines A: the distribution of heterozygous genotype rate of the 290 maize hybrid lines; B: the distribution of different locus number obtained by pairwise comparative analysis in 290 maize hybrid lines."

Table 2

Comparison of genetic diversity among six maturation groups"

熟期
Mature stage		 样品数量
Number		 总基因型数
Number of
genotypes		 总等位基因数
Number of
alleles		 杂合基因型频率平均值
Heterozygous
genotype rate		 基因多样性
Gene diversity
极早熟 Extremely early maturity 11 263 210 0.66 0.66
早熟 Early maturity 25 458 291 0.61 0.69
中早熟 Mid-early maturity 38 492 291 0.65 0.67
中熟 Mid-maturity 86 753 389 0.66 0.68
中晚熟 Mid-late maturity 122 717 359 0.67 0.65
晚熟 Late maturity 8 215 193 0.66 0.64

Fig. 2

Phylogenetic tree of 295 maize varieties Red: extremely early maturity (JZS); yellow: early maturity (ZS); brown: mid-early maturity (ZZS); blue: mid-maturity (ZHS); light green: mid-late maturity (ZWS); black: late maturity (WS). The black circles represent the representative varieties in Fig. 2."

Fig. 3

PCoA analysis of 295 maize varieties A: the relationship among three types varieties; B: the relationship among seven groups of late maturing groups; the black hollow circles represent the representative varieties."

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