Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (10): 1844-1850.doi: 10.3724/SP.J.1006.2009.01844

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Identification of Phytophthora Resistance Gene in Soybean Cultivar Yudou25

FAN Ai-Ying1,2,WANG Xiao-Ming1,FANG Xiao-Ping2,WU Xiao-Fei1,ZHU Zhen-Dong1*   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Genetic Resources and Improvement, Beijing 100081, China; 2 Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Received:2009-02-08 Revised:2009-05-31 Online:2009-10-12 Published:2009-08-07
  • Contact: ZHU Zhen-Dong,E-mail: zhuzd115@caas.net.cn

Abstract:

Phytophthora root rot, caused by Phytophthora sojae, is a destructive disease on soybean. Use of resistant soybean cultivars is the most economical and effective method for controlling the disease. Up to now, nine loci for the resistance with 15 genes have been identified in soybean. However, only a few genes, such as Rps1c and Rps1k,were effectively resistant to populations of P. sojae in China, so mining new resistance genes is necessary greatly for the disease control. Soybean cv. Yudou 25 has broad spectrum resistance to P. sojae, and is an elite resistance source for Phytophthora root rot of soybean. To effectively utilize the cultivar in resistance breeding, in the present study, we identified and tagged the Phytophthora resistance gene in the cultivar by using SSR markers and bulked segregation analysis (BSA). Two F2:3 populations were developed for resistance genetic analysis and resistance gene mapping. Using hypocotyls inoculation technique at the seedling stage in the glasshouse, the reaction to P. sojae isolate PSMC1 (virulence type 1b, 1d, 3a, 3b, 3c, 4, 5, 6, 7) in 82 and 98 F2:3 families derived from two crosses of Yudou 21×Yudou 25 and Zaoshu 18×Yudou 25, respectively, were identified. The segregation ratio in both populations fit into 1:2:1 for homozygous resistant, segregating and homozygous susceptible, showing that the cultivar resistance to Phytophthora root rot is controlled by a dominant single gene, with the temporary name of RpsYD25. On the basis of linkage analysis with SSR markers, RpsYD25 was located on soybean molecular linkage group (MLG) N in both populations. Five SSR markers were associated with RpsYD25 in an order of Sat_208-Satt530-RpsYD25-Sat_084-Satt125-Sat_236 in F2:3 population from the cross of Yudou 21×Yudou 25, RpsYD25 was flanked by Satt530 and Sat_084 with a distance of 6.3 and 7.7 cM, respectively. Five SSR markers were linked to RpsYD25 in an order of Satt125-RpsYD25-Sat_275-Sat_266-Satt660-GMABAB in F2:3 population from the cross of Zaoshu 18×Yudou 25, RpsYD25 was flanked by Satt125 and Sat_275 with a distance of 7.9 and 7.8 cM, respectively. Because RpsYD25 was mapped on MLG N near to Rps1 locus, the genetic relationship of RpsYD25 and Rps1 was detected by using the selected SSR markers contained in the Rps1k allele sequence. A SSR marker Rps1k6 in Rps1k allele was found to be linked to RpsYD25 with a genetic distance of 19.4 cM in F2:3 population from the crosses Yudou 21×Yudou 25. Therefore, the Phytophthora resistance gene RpsYD25 in cv. Yudou 25 might be a novel allele at Rps1 locus, or a novel gene.

Key words: Glycine max, Phytophthora root rot, Phytophthora sojae, Resistance gene, SSR marker

[1] Han X-Z(韩晓增), He Z-H(何志鸿), Zhang Z-M(张增敏). Methods for controlling major diseases and pests in soybean. Soybean Bull (大豆通报), 1998, (6): 5-6 (in Chinese)

[2] Ma S-M(马淑梅), Ma C-Y(马成云), Yao W-Q(姚文秋). The investigation on the region harmed by soybean Phytophthora megasperma f. sp. glycinea kuan & Erwin and the research of pathogenicity diversity. Agric Technol (农业与技术), 2007, 27(2): 41-44 (in Chinese with English abstract)

[3] Chen Q-H(陈庆河), Weng Q-Y(翁启勇), Wang Y-C(王源超), Zheng X-B(郑小波). Identification and sequencing of ribosomal DNA-ITS of Phytophthora sojae in Fujian. Acta Phytopathol Sin (植物病理学报), 2004, 34(2): 112-116 (in Chinese with English abstract)

[4] Zhu Z-D(朱振东), Wang H-B(王化波), Wang X-M(王晓鸣), Chang R-Z(常汝镇), Wu X-F(武小菲). Distribution and virulence diversity of Phytophthora sojae in China. Sci Agric Sin (中国农业科学), 2003, 36(7): 793-799 (in Chinese with English abstract)

[5] Wang H(王华), Li G-Y(李国英), Zhan Y(战勇), Wang P(王朴), Zhang P(张萍). Identification of Phytophthora root rot of soybean in Xinjiang. Xinjiang Agric Sci (新疆农业科学), 2006, 43(2): 106-108 (in Chinese with English abstract)

[6] Schmitthenner A F. Problems and progress in control of Phytophthora root rot of soybean. Plant Dis, 1985, 69: 362-368

[7] Zhu Z-D(朱振东), Wang X-M(王晓鸣), Chang R-Z(常汝镇), Ma S-M(马淑梅), Wu X-F(武小菲), Tian Y-L(田玉兰). Identification of races of Phytophthora sojae and reaction of soybean germplasm resources in Heilongjiang province. Sci Agric Sin (中国农业科学), 2000, 33(1): 62-67 (in Chinese with English abstract)

[8] Chen X-L(陈晓玲), Zhu Z-D(朱振东), Wang X-M(王晓鸣), Xiao Y-N(肖炎农), Wu X-F(武小菲). Postulation of Phytophthora resistance genes in soybean cultivars or lines. Sci Agric Sin (中国农业科学), 2008, 41(4): 1227-1234 (in Chinese with English abstract)

[9] Zhu Z-D(朱振东), Huo Y-L(霍云龙), Wang X-M(王晓鸣), Huang J-B(黄俊斌), Wu X-F(武小菲). Screening for resistance sources to Phytophthora root rot in soybean. J Plant Genet Resour (植物遗传资源学报), 2006, 7(1): 24-30 (in Chinese with English abstract)

[10] Wang X-M(王晓鸣), Zhu Z-D(朱振东), Wang H-B(王化波), Wu X-F(武小菲), Tian Y-L(田玉兰). The resistance of soybean germplasm to Phytophthora root rot. J Plant Genetic Resour (植物遗传资源科学), 2001, 2(2): 22-26 (in Chinese with English abstract)

[11] Zhang S-Z(张淑珍), Wu J-J(吴俊江), Xu P-F(徐鹏飞), Li W-B(李文滨), Zuo Y-H(左豫虎), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Chen C(陈晨), Wang J-S(王金生), Yu A-L(于安亮), Jin L-M(靳立梅). Identification of virulence Phytophthora sojae in Heilongjiang province and the first report on race 15 in China. Chinese J Oil Crop Sci (中国油料作物学报), 2008, 30: 229-234 (in Chinese with English abstract)

[12] Li W-D(李卫东), Liang H-Z(梁慧珍), Lu W-G(卢为国), Xu J-J(许景菊). Several trait analysis of genetic background and yield of soybean Yudou 25. Seed (种子), 1999, (4): 78-79 (in Chinese)

[13] Gordon S G, St Martin S K, Dorrance A E. Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci, 2006, 46: 168-173

[14] Sharp P J, Kreis M, Shewry P R, Gale M. Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet, 1988, 75: 289-290

[15] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating population. Proc Natl Acad Sci USA, 1991, 88: 9828-9832

[16] Demirbas A, Rector B G, Lohnes D G, Fioritto R J, Graef G L, Cregan P B, Shoemaker R C, Specht E. Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance. Crop Sci, 2001, 41: 1220-1227

[17] Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: An interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics, 1987, 1: 174-181

[18] Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1944, 12: 172-175

[19] Bernard R L, Smith P E, Kaufmann M J, Schmitthenner A F. Inheritance of resistance to Phytophthora root and stem rot in the soybean. Agron J, 1957, 49: 391

[20] Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregan P B. A new integrated genetic linkage map of the soybean. Theor Appl Genet, 2004, 109: 122-128

[21] Cregan P B, Jarvik T, Bush A L, Shoemaker R C, Lark K G, Kahler A L, Kaya N, VanToai T T, Lohnes D G, Chung J, Specht J E. An integrated genetic linkage map of the soybean genome. Crop Sci, 1999, 39: 1464-1490

[22] Sandhu D, Schallock K G, Rivera-Velez N, Lundeen P, Cianzio S, Bhattacharyya M K. Soybean Phytophthora resistance gene Rps8 maps closely to the Rps3 region. J Hered, 2005, 96: 536-541

[23] Sugimoto T, Yoshida S, Watanabe K, Aino M, Kanto T, Maehawa K, Irie K. Identification of SSR markers linked to the Phytophthora resistance gene Rps1-d in soybean. Plant Breed, 2008, 127: 154-159

[24] Weng C, Yu K, Anderson T R, Poysa V. Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J Hered, 2001, 92: 442-446

[25] Bertrand C Y C, Mackill D J. Marker-assisted selection: An approach for precision plant breeding in the twenty-first century. Philos Trans R Soc Lond B Biol Sci, 2008, 363: 557-572

[26] Bhattacharyya M K, Narayanan N N, Gao H, Santra D K, Salimath S S, Kasuga T, Liu Y, Espinosa B, Ellison L, Marek L, Shoemaker R, Gijzen M, Buzzell R I. Identification of a large cluster of coiled coil-nucleotide binding site-leucine rich repeat-type genes from the Rps1k region containing Phytophthora resistance genes in soybean. Theor Appl Genet, 2005, 111: 75-86

[27] Kasuga T, Salimath S S, Shi J, Gijzen M, Buzzell R I, Bhattacharyya M K. High resolution genetic and physical mapping of molecular markers linked to the Phytophthora resistance gene Rps1-k in soybean. Mol Plant Microbe Interact, 1997, 10: 1035-1044
Gao H, Bhattacharyya M. The soybean Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binging-leucine rich repeat-like genes and repetitive sequences. BMC Plant Biol, 2008, 8: 29
[1] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[2] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[3] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[4] JIANG Wei, PAN Zhe-Chao, BAO Li-Xian, ZHOU Fu-Xian, LI Yan-Shan, SUI Qi-Jun, LI Xian-Ping. Genome-wide association analysis for late blight resistance of potato resources [J]. Acta Agronomica Sinica, 2021, 47(2): 245-261.
[5] ZHANG Xue-Cui, SUN Su-Li, LU Wei-Guo, LI Hai-Chao, JIA Yan-Yan, DUAN Can-Xing, ZHU Zhen-Dong. Identification of resistance gene against phytophthora root rot in new soybean lines breeded in Henan province [J]. Acta Agronomica Sinica, 2021, 47(2): 275-284.
[6] ZHANG Rong-Yue, WANG Xiao-Yan, YANG Kun, SHAN Hong-Li, CANG Xiao-Yan, LI Jie, WANG Chang-Mi, YIN Jiong, LUO Zhi-Ming, LI Wen-Feng, HUANG Ying-Kun. Identification of brown rust resistance and molecular detection of Bru1 gene in new and main cultivated sugarcane varieties [J]. Acta Agronomica Sinica, 2021, 47(2): 376-382.
[7] ZHANG Huan, LUO Huai-Yong, LI Wei-Tao, GUO Jian-Bin, CHEN Wei-Gang, ZHOU Xiao-Jing, HUANG Li, LIU Nian, YAN Li-Ying, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang. Genome-wide identification of peanut resistance genes and their response to Ralstonia solanacearum infection [J]. Acta Agronomica Sinica, 2021, 47(12): 2314-2323.
[8] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[9] WEN Jing, SHEN Yan-Qi, HAN Si-Ping, XING Yue-Xian, ZHANG Ye, WANG Zi-Yu, LI Shi-Jie, YANG Xiao-Hong, HAO Dong-Yun, ZHANG Yan. Exploration of specific gene(s) for ear rot resistance to Fusarium verticilloides in maize [J]. Acta Agronomica Sinica, 2020, 46(9): 1303-1311.
[10] ZHANG Xue-Cui,ZHONG Chao,DUAN Can-Xing,SUN Su-Li,ZHU Zhen-Dong. Fine mapping of Phytophthora resistance gene RpsZheng in soybean cultivar Zheng 97196 [J]. Acta Agronomica Sinica, 2020, 46(7): 997-1005.
[11] Wen-Yang XIANG,Yong-Qing YANG,Qiu-Yan REN,Tong-Tong JIN,Li-Qun WANG,Da-Gang WANG,Hai-Jian ZHI. Cloning and analysis of candidate gene resistant to SC3 in soybean [J]. Acta Agronomica Sinica, 2019, 45(12): 1822-1831.
[12] ZHANG An-Ning,LIU Yi,WANG Fei-Ming,XIE Yue-Wen,KONG De-Yan,NIE Yuan-Yuan,ZHANG Fen-Yun,BI Jun-Guo,YU Xin-Qiao,LIU Guo-Lan,LUO Li-Jun. Pyramiding and evaluation of brown planthopper resistance genes in water-saving and drought-resistance restorer line [J]. Acta Agronomica Sinica, 2019, 45(11): 1764-1769.
[13] CHEN Fang,QIAO Lin-Yi,LI Rui,LIU Cheng,LI Xin,GUO Hui-Juan,ZHANG Shu-Wei,CHANG Li-Fang,LI Dong-Fang,YAN Xiao-Tao,REN Yong-Kang,ZHANG Xiao-Jun,CHANG Zhi-Jian. Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357 [J]. Acta Agronomica Sinica, 2019, 45(10): 1503-1510.
[14] XUE Yan-Tao,LU Ping,SHI Meng-Sha,SUN Hao-Yue,LIU Min-Xuan,WANG Rui-Yun. Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu [J]. Acta Agronomica Sinica, 2019, 45(10): 1511-1521.
[15] Xiang-Yi XIAO,Xue-Tao SHI,Hao-Wen SHENG,Jin-Ling LIU,Ying-Hui XIAO. Fine Mapping and Candidate Gene Analysis of Rice Blast Resistance Gene Pi47 [J]. Acta Agronomica Sinica, 2018, 44(7): 977-987.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!