Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (4): 539-548.doi: 10.3724/SP.J.1006.2010.00539

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Cloning and Analysis of GmCIL4 Gene in Glycine max L.

ZHANG Qing-Zhe1,MA Jin-Hua1,CHEN Xin-Jian1,*,FU Yong-Fu2,*   

  1. 1 College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China; 2 Institute of Crop Sciences, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 10081, China
  • Received:2009-07-28 Revised:2009-12-10 Online:2010-04-12 Published:2010-02-05
  • Contact: CHEN Xin-Jian, E-mail: xinjian@371.net;FU Yong-Fu, E-mail: fuyf@caas.net.cn

Abstract:

The model plant Arabosopsis thaliana has been identified to have four major flowering pathways. Among them, the photoperiod pathway integrates the light signal including light/dark cycle, light spectrum, light intensity, and light duration, to mediate flower initiation. CONSTANS(CO) is a key gene in photoperiodic flowering pathway and acts between genes of the circadian clock and meristem identity. CO encodes a protein containing two zinc finger regions (B-box I and II) near the amino terminus and a CCT (CO, CO-Like, TOC1) domain near the carboxy terminus. The CO from Arabidopsis thaliana is one member of the family comprised of 17 members, which can be classed into three subgroups based on their characters of functional domains. To elucidate the function of CO in flowering in soybean, cloned one of CO-like gene, named as GmCOL4, from Glycine max L. Kennong 18. Bioinformatics analysis revealed that GmCOL4 encoded a protein embedded two conserved domains, B-box and CCT, and belonged to subgroup III. Phylogenetic analysis based on the critical amino acid sequences indicated that GmCOL4 was much close to COL9 with similarity of 64.3%. The expression profiles of GmCOL4 by quantitative real-time RT-PCR (qRT-PCR) showed a similar pattern to that of COL9. GmCOL4 was largely regulated by biological circadian, while the light appeared weak effect on it. And GmCOL4 expressed mainly in leaves and had its highest amount in anthesis. The results suggested that GmCOL4 is one of the important genes in the regulation of flowering and photoperiodic in soybean. It paves a way to study the function of CO family in soybean and its application in soybean molecular breeding.

Key words: CINSTANS, Bioinformatics, Circadian clock, Photoperiod, Glycine max L.

 


[1] G arner W W, Allard H A. Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants. J Agric Res, 1920, 18: 553-606

[2] Shaw D, Goldman B D. Gender differences in influence of prenatal photoperiods on postnatal pineal melatonin rhythms and serum prolactin and follicle-stimulating hormone in the Siberian hamster(Phodopus sungorus). Endocrinology, 1995, 136: 4237-4246

[3] Putterill J, Robson F, Lee K, Simon R, Coupland G. The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell, 1995, 80: 847-857

[4] Suarez-Lopez P, Wheatley K, Robson F, Onouchi H, Valverde F, Coupland G. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature, 2001, 410: 1116-1120

[5] Imaizumi T, Schultz T F, Harmon F G, Ho L A, Kay S A. FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis. Science, 2005, 309: 293-297

[6] Sawa M, Nusinow D A, Kay S A, Imaizumi T. FKF1 and GIGANTEA complex formation is required for day-length mea- surement in Arabidopsis. Science, 2007, 318: 261-265

[7] Valverde F, Mouradov A, Soppe W, Ravenscroft D, Samach A, Coupland G. Photoreceptor regulation of CONSTANS protein in photoperiodic flowering. Science, 2004, 303: 1003-1006

[8] Kardailsky I, Shukla V K, Ahn J H, Dagenais N, Christensen S K, Nguyen J T, Chory J, Harrison M J, Weigel D. Activation tagging of the floral inducer FT. Science, 1999, 286: 1962-1965

[9] Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T. A pair of related genes with antagonistic roles in mediating flowering signals. Science, 1999, 286: 1960-1962

[10] Onouchi H, Igeno M I, Perilleux C, Graves K, Coupland G. Mutagenesis of plants overexpressing CONSTANS demonstrates novel interactions among Arabidopsis flowering-time genes. Plant Cell, 2000, 12: 885-900

[11] Samach A, Onouchi H, Gold S E, Ditta G S, Schwarz-Sommer Z, Yanofsky M F, Coupland G. Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science, 2000, 288: 1613-1616

[12] Borden K L. RING fingers and B-boxes: Zinc-binding protein-protein interaction domains. Biochem Cell Biol, 1998, 76: 351-358

[13] Strayer C, Oyama T, Schultz T F, Raman R, Somers D E, Mas P, Panda S, Kreps J A, Kay S A. Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog. Science, 2000, 289: 768-771

[14] Torok M, Elkin L D. Two B or not two B? Overview of the rapidly expanding B-box family of proteins. Differentiation, 2000, 67: 63-71

[15] Robson F, Costa M M, Hepworth S R, Vizir I, Pineiro M, Reeves P H, Putterill J, Coupland G. Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants. Plant J, 2001, 28: 619-631

[16] Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 2000, 12: 2473-2484

[17] Nemoto Y, Kisaka M, Fuse T, Yano M, Ogihara Y. Characterization and functional analysis of three wheat genes with homology to the CONSTANS flowering time gene in transgenic rice. Plant J, 2003, 36: 82-93

[18] Serrano G, Herrera-Palau R, Romero J M, Serrano A, Coupland G, Valverde F. Chlamydomonas CONSTANS and the evolution of plant photoperiodic signaling. Curr Biol, 2009, 19: 359-368

[19] Griffiths S, Dunford R P, Coupland G, Laurie D A. The evolution of CONSTANS-like gene families in barley, rice, and Arabidopsis. Plant Physiol, 2003, 131: 1855-1867

[20] Robert L S, Robson F, Sharpe A, Lydiate D, Coupland G. Conserved structure and function of the Arabidopsis flowering time gene CONSTANS in Brassica napus. Plant Mol Biol, 1998, 37: 763-772

[21] Holefors A, Opseth L, Ree Rosnes A K, Ripel L, Snipen L, Fossdal C G, Olsen J E. Identification of PaCOL1 and PaCOL2, two CONSTANS-like genes showing decreased transcript levels preceding short day induced growth cessation in Norway spruce. Plant Physiol Biochem, 2009, 47: 105-115

[22] Hu R-B(胡瑞波). Molecular Cloning, Expression Profiles and Functional Analysis of FT/TFL1 Genes in Soybean (Glycine max). PhD Dissertation of Chinese Academy of Agricultural Sciences, 2009 (in Chinese with English Abstract)

[23] Chen Q J, Zhou H M, Chen J, Wang X C. Using a modified TA cloning method to create entry clones. Anal Biochem, 2006, 358: 120-125 Cheng X F, Wang Z Y. Overexpression of COL9, a CONSTANS- LIKE gene, delays flowering by reducing expression of CO and FT in Arabidopsis thaliana. Plant J, 2005, 43: 758-768
[1] XU Xin, QIN Chao, ZHAO Tao, LIU Bin, LI Hong-Yu, LIU Jun. Function analysis of GmELF3s in regulating soybean flowering time and circadian rhythm [J]. Acta Agronomica Sinica, 2022, 48(4): 812-824.
[2] WU Yan-Fei, HU Qin, ZHOU Qi, DU Xue-Zhu, SHENG Feng. Genome-wide identification and expression analysis of Elongator complex family genes in response to abiotic stresses in rice [J]. Acta Agronomica Sinica, 2022, 48(3): 644-655.
[3] JIAN Hong-Ju, SHANG Li-Na, JIN Zhong-Hui, DING Yi, LI Yan, WANG Ji-Chun, HU Bai-Geng, Vadim Khassanov, LYU Dian-Qiu. Genome-wide identification and characterization of PIF genes and their response to high temperature stress in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 86-98.
[4] HUANG Ning, HUI Qian-Long, FANG Zhen-Ming, LI Shan-Shan, LING Hui, QUE You-Xiong, YUAN Zhao-Nian. Identification, localization and expression analysis of beta-carotene isomerase gene family in sugarcane [J]. Acta Agronomica Sinica, 2021, 47(5): 882-893.
[5] LI Peng, LIU Che, SONG Hao, YAO Pan-Pan, SU Pei-Lin, WEI Yao-Wei, YANG Yong-Xia, LI Qing-Chang. Identification and analysis of non-specific lipid transfer protein family in tobacco [J]. Acta Agronomica Sinica, 2021, 47(11): 2184-2198.
[6] HUANG Xiao-Fang,BI Chu-Yun,SHI Yuan-Yuan,HU Yun-Zhuo,ZHOU Li-Xiang,LIANG Cai-Xiao,HUANG Bi-Fang,XU Ming,LIN Shi-Qiang,CHEN Xuan-Yang. Discovery and analysis of NBS-LRR gene family in sweet potato genome [J]. Acta Agronomica Sinica, 2020, 46(8): 1195-1207.
[7] JIA Xiao-Ping,YUAN Xi-Lei,LI Jian-Feng,WANG Yong-Fang,ZHANG Xiao-Mei,ZHANG Bo,QUAN Jian-Zhang,DONG Zhi-Ping. Photo-thermal interaction model under different photoperiod-temperature conditions and expression analysis of SiCCT gene in foxtail millet (Setaria italica L.) [J]. Acta Agronomica Sinica, 2020, 46(7): 1052-1062.
[8] ZHENG Qing-Lei,YU Chen-Jing,YAO Kun-Cun,HUANG Ning,QUE You-Xiong,LING Hui,XU Li-Ping. Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC [J]. Acta Agronomica Sinica, 2020, 46(6): 844-857.
[9] Fang-Quan WANG,Fang-Jun FAN,Shi-Jian XIA,Shou-Yu ZONG,Tian-Qing ZHENG,Jun WANG,Wen-Qi LI,Yang XU,Zhi-Hui CHEN,Yan-Jie JIANG,Ya-Jun TAO,Wei-Gong ZHONG,Jie YANG. Interactive effects of the photoperiod-/thermo-sensitive genic male sterile genes tms5 and pms3 in rice [J]. Acta Agronomica Sinica, 2020, 46(3): 317-329.
[10] YAO Jun-Yue,HUA Ying-Peng,ZHOU Ting,WANG Tao,SONG Hai-Xing,GUAN Chun-Yun,ZHANG Zhen-Hua. Identification and function analysis of AVP1, VHA-a2, and VHA-a3 genes in Brassica napus L. [J]. Acta Agronomica Sinica, 2019, 45(8): 1146-1157.
[11] SUN Ting-Ting,WANG Wen-Ju,LOU Wen-Yue,LIU Feng,ZHANG Xu,WANG Ling,CHEN Yu-Feng,QUE You-Xiong,XU Li-Ping,LI Da-Mei,SU Ya-Chun. Cloning and expression analysis of sugarcane lipoxygenase gene ScLOX1 [J]. Acta Agronomica Sinica, 2019, 45(7): 1002-1016.
[12] JIA Xiao-Ping,QUAN Jian-Zhang,WANG Yong-Fang,DONG Zhi-Ping,YUAN Xi-Lei,ZHANG Bo,LI Jian-Feng. Effects of different photoperiod conditions on agronomic traits of foxtail millet [J]. Acta Agronomica Sinica, 2019, 45(7): 1119-1127.
[13] Gui-Hong LIANG,Ying-Peng HUA,Ting ZHOU,Qiong LIAO,Hai-Xing SONG,Zhen-Hua ZHANG. Bioinformatics analysis and response to nitrate-cadmium stress of NRT1.5 and NRT1.8 family genes in Brassica napus [J]. Acta Agronomica Sinica, 2019, 45(3): 365-380.
[14] Kun GAO,Ying-Peng HUA,Hai-Xing SONG,Chun-Yun GUAN,Zhen-Hua ZHANG,Ting ZHOU. Identification and Bioinformatics Analysis of the PIN Family Gene in Brassica napus [J]. Acta Agronomica Sinica, 2018, 44(9): 1334-1346.
[15] Zuo-Min WANG,Jin LIU,Shi-Chao SUN,Xin-Yu ZHANG,Fei XUE,Yan-Jun LI,Jie SUN. Identification and Expression Analysis of Multidrug and Toxic Compound Extrusion Protein Family Genes in Colored Cotton [J]. Acta Agronomica Sinica, 2018, 44(9): 1380-1392.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!