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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (4): 539-548.doi: 10.3724/SP.J.1006.2010.00539

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Cloning and Analysis of GmCIL4 Gene in Glycine max L.

ZHANG Qing-Zhe1,MA Jin-Hua1,CHEN Xin-Jian1,*,FU Yong-Fu2,*   

  1. 1 College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China; 2 Institute of Crop Sciences, National Key Facility of Crop Gene Resource and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 10081, China
  • Received:2009-07-28 Revised:2009-12-10 Online:2010-04-12 Published:2010-02-05
  • Contact: CHEN Xin-Jian, E-mail: xinjian@371.net;FU Yong-Fu, E-mail: fuyf@caas.net.cn

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Abstract:

The model plant Arabosopsis thaliana has been identified to have four major flowering pathways. Among them, the photoperiod pathway integrates the light signal including light/dark cycle, light spectrum, light intensity, and light duration, to mediate flower initiation. CONSTANS(CO) is a key gene in photoperiodic flowering pathway and acts between genes of the circadian clock and meristem identity. CO encodes a protein containing two zinc finger regions (B-box I and II) near the amino terminus and a CCT (CO, CO-Like, TOC1) domain near the carboxy terminus. The CO from Arabidopsis thaliana is one member of the family comprised of 17 members, which can be classed into three subgroups based on their characters of functional domains. To elucidate the function of CO in flowering in soybean, cloned one of CO-like gene, named as GmCOL4, from Glycine max L. Kennong 18. Bioinformatics analysis revealed that GmCOL4 encoded a protein embedded two conserved domains, B-box and CCT, and belonged to subgroup III. Phylogenetic analysis based on the critical amino acid sequences indicated that GmCOL4 was much close to COL9 with similarity of 64.3%. The expression profiles of GmCOL4 by quantitative real-time RT-PCR (qRT-PCR) showed a similar pattern to that of COL9. GmCOL4 was largely regulated by biological circadian, while the light appeared weak effect on it. And GmCOL4 expressed mainly in leaves and had its highest amount in anthesis. The results suggested that GmCOL4 is one of the important genes in the regulation of flowering and photoperiodic in soybean. It paves a way to study the function of CO family in soybean and its application in soybean molecular breeding.

Key words: CINSTANS, Bioinformatics, Circadian clock, Photoperiod, Glycine max L.

 


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