Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (07): 1169-1175.doi: 10.3724/SP.J.1006.2010.01169

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Antioxidative Activities of Transgenic Yeast with Ferritin Gene from Wheat

ZHAO Yong-Liang1,2,CHEN Jing1,WANG Dan1,WANG Wei-Guo1, LI Yun-Hui1   

  1. 1Department of Biology Engineering,Henan University of Technology,Zhengzhou,45001,China;2The National Key Facility for Crop Gene Resources and Genetic Improvement,Key Laboratory of Crop Germplasm and Biotechnology,Ministry of Agriculture,Institute of Crop Sciences,Chinese Academy of Agricultural Sciencses,Beijing 100081.China
  • Received:2009-12-17 Revised:2010-03-19 Online:2010-07-12 Published:2010-04-28

PDF

1486

Abstract: Ferritin, a key protein to store iron, is widely distributed in animal, plant, and microbe. Ferritin can regulate the balance in iron metabolism and decrease the oxidative damage caused by ferrous ion. It is generally recognized that the antioxidant activity of ferritin is mainly caused by integrating ferrous ion among cells, and thereby decreasing the generation of Fenton reaction. In addition to the antioxidant pathway against hydrogen peroxide (H2O2), less information is available on other antioxidative pathways and mechanism of ferritin. In this study, a ferritin gene was cloned from wheat (Triticum aestivum L.), and its expression vehicle of eukaryotic cell was constructed and transformed into yeast (Saccharomyces cerevisiae). Compared with the control yeasts, the transgenic yeasts were able to scavenge H2O2,O2?and ·OH effectively. The half lethal density (LD50) of transgenic yeasts induced by the three types of active oxygen (·OH, O2?and H2O2) was 0.40, 1.00, and 36.91 mmol L-1, which increasedby37.93%, 47.06%, and 77.03% compared with the control respectively. The abilities for eliminating ·OH, O2?, and H2O2 of the trsnsgenic yeast per unit concentiation were quantitated with cubic polynomial, logarithmic, and negative exponential equations based on yeast concentration respectively. These results demonstrated that expressed products of wheat ferritin gene in eukaryotic orgamism can effectivly in eliminate various types of active oxygen with different and complex mechanisms.

Key words: Wheat, Ferritin, Trangenic yeast, Antioxdative activity

[1]    Zheng Y-L(郑荣梁), Huang Z-Y(黄中洋). Free Radical Biology (自由基生物学), 3rd edn. Beijing: Higher Education Press, 2007. pp 1–25 (in Chinese)
[2]    Blokhina O, Virolatinen E, Kurt V F. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot, 2003, 91: 179–194
[3]    Theil E C, Matzapetakis M, Liu X. Ferritins: Iron/oxygen biominerals in protein nanocages. J Biol Inorg Chem, 2006, 11, 803–810
[4]    Harrison P M, Arosio P. Ferritins: molecular properties, iron storage function and cellular regulation.  Biochim Biophys Acta,1996, 1275: 161–203
[5]    Theil, E C. Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms. Annu Rev Biochem, 1987, 56: 289–315
[6]    Wade V J, Treffry A, Laulhere J P, Bauminger E R, Cleton M I, Mann S, Briat J F, Harrison P M. Structure and composition of ferritin cores from pea seed (Pisum sativum). Biochim Biophys Acta, 1993, 1161: 91–96
[7]    Goto F, Yoshihara T, Saiki H, Takaiwa F. Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theor Appl Genet,2000, 100: 658–664
[8]    Ye X(叶霞), Huang X-D(黄晓德张振).Agrobacterium-mediated transformation of tomato with PvFer. Acta Hort Sin (园艺学报), 2007, 34(2): 489–492 (in Chinese with English abstract)), Yao H-Q(姚泉洪), Zhang Z(
[9]    Ye H X, Li M, Guo Z J. Evaluation and application of two high-Iron transgenic rice lines expressing a pea ferritin gene. Rice Sci, 2008, 15: 51–56
[10] Deak M, Horvath G V, Davletova S, Torok K, Sass L, Vass I, Barna B, Kiraly Z, Dudits D. Plants ectopically expressing the iron-binding protein, ferritin, are tolerant to oxidative damage and pathogens. Nat Biotechnol, 1999, 17: 192–196
[11] Hegedus A, Janda T, Horváth G VDudits D. Accumulation of overproduced ferritin in the chloroplast provides protection against photoinhibition induced by low temperature in tobacco plants. J Plant Physiol, 2008, 165: 1647–1651,
[12] Halliwell B, Gutteridge J M. Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity. In: Free Radicals in Biology and Medicine. New York: Oxford University Press, 1989. pp 86–179
[13] Balla G, Jacob H S, Balla J, Rosenberg M, Nath K, Apple F, Eaton J W, Vercellotti G M. Ferritin: a cytoprotective antioxidant strategem of endothelium. J Biol Chem, 1992, 267: 18148–18153
[14] Baldi A, Lombardi D, Russo P ,Palescandolo Ferritin contributes to melanoma progression by modulating cell growth and sensitivity to oxidative stress., 2005, 11: 3175–3183 E, Luca A D, Santini D, Baldi F, Rossiello L, Dellanna M L, Mastrofrancesco A, Maresca V, Flori E, Natali P G, Picardo Mauro, Pagg M G.Clin Cancer Res
[15] Pham C G Papa S, Jones J, Alvarez K, Jayawardena S, Smaele D E, Cong R, Beaumont C. Ferritin heavy chain upregulation by NF-kappaB inhibits TNF alpha-induced apoptosis by suppressing reactive oxygen species. , 2004, 119: 529, Bubici C, Zazzeroni F,Cell–542
[16] Ravet K, Touraine B, Boucherez J, Briat J F, Gaymard F, Cellier F. Ferritins control interaction between iron homeostasis and oxidative stress in Arabidopsis. Plant J, 2009, 57: 400–412
[17] Kim H J, Kim H M, Kim J H, Ryu K S, Park S M, Jahng K Y, Yang M K, Kim D H. Expression of heteropolymeric ferritin improves iron storage in Saccharomyces cerevisiae. Appl Environ Microbiol, 2003, 69: 1999–2005
[18] Shin Y M, Kwon T H, Kim K S, Chae K S, Kim D H, Kim J H, Yang M S.Enhanced iron uptake of Saccharomyces cerevisiae by heterologous expression of a tadpole ferritin gene. Appl Environ Microbiol, 2001, 67: 1280–1283
[19] Campanella A, Isaya G, O’Neill H A, Santambrogio P, Cozzi A, Arosio P, Levi Sonia. The expression of human mitochondrial ferritin rescues respiratory function in frataxin-deficient yeast. Human Mol Genet, 2004, 13: 2279–2288
[20] Zhao Y-L(赵永亮), Zhou R-H(周荣华), Jia J-Z(贾继增). The homologous cloning of the ferritin gene from wheat. J Triticeae Crops (麦类作物学报), 2008, 28(1): 25–30 (in Chinese with English abstract)
[21] Schiestl R H, Gietz R D. High efficiency transformation of intact yeast cells using single stranded DNA as a carrier. Currt Genet, 1989, 16: 339–346
[22] Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680–685
[23] Barcelo F, Arean C O, Moore G R. Isolation and preliminary characterization of ferritin from clover seeds. BioMetals, 1995, 8: 47–52
[24] Bradford M M. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem, 1976, 72: 248–254
[25] Jin M(金鸣), Cai Y-X(蔡亚欣), Li J-R(李金荣), Zhao H(赵辉). 1,10-phenanthroline-Fe(2+) oxidative assay of hydroxyl radical produced by H2O2/Fe(2+). Prog Biochem Biophys (生物化学与生物物理进展), 1996, 23(6): 553–555 (in Chinese with English abstract)
[26] Marlund S, Marlund G. Involvement of superoxide anion radicals in the autoxidation of pyrogallol and a convininent assay for superoxide dismutase. Eur J Biochem,1974, 47: 469–471
[27] Zhang H-R(张海容). Study of the scavenging radical activities of polysaccharide of hippophae rhamnosides fruit peel. Bull Bot (植物学通报), 2005, 22(6): 703–707 (in Chinese with English abstract)
[28] Cai H-J(蔡华静), Xiong Z-Q(熊智强), Tu G-Q(涂国全). A preliminary study on the antibacterial action of the bioactive compound produced by streptomyces 702. Acta Agric Univ Jiangxiensis (江西农业大学学报), 2005, 27(2): 274–278 (in Chinese with English abstract)
[29] Wang Y-H(王义华), Xu M-Z(徐梅珍), Jiang P(江萍), He Z-F(何照范), Xiong L-Y(熊绿芸).Purification, chemical component and antioxidative analysis of polysaccharide from Saccharomyces cerevisiae.Microbiology (微生物学通报), 2003, 30(4): 51–53 (in Chinese with English abstract)
[30] Sheng W(盛玮).Study on glutathione peroxidese activity in yeast.J Huaibei Ind Teachers Coll (淮北煤炭师范学院学报), 2003, 24(1): 40–43 (in Chinese with English abstract)
[31] Lin Z-L(林稚兰), Hao F-Y(郝福英), Wang L(王龙), Jia L(贾乐), Tian F(田枫), Li Q(李奇). Study on antioxidation of Saccharomyces cerevisiae Cu-metallothionein. Mycosystema (菌物学报), 1997, 15(4): 291–296 (in Chinese with English abstract) Zhang K-S(张坤生), Zhang L(张亮), Lü X-L(吕晓玲), Chen X-J(陈晓娟). The study on antioxidant activity of Rhodotorular glutinis pigment. Food Sci(食品科学), 2004, 25(3): 61–63 (in Chinese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[5] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[6] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[7] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[8] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[9] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[10] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[11] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[12] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[13] WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[14] LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
[15] LUO Jiang-Tao, ZHENG Jian-Min, PU Zong-Jun, FAN Chao-Lan, LIU Deng-Cai, HAO Ming. Chromosome transmission in hybrids between tetraploid and hexaploid wheat [J]. Acta Agronomica Sinica, 2021, 47(8): 1427-1436.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd