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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (07): 1169-1175.doi: 10.3724/SP.J.1006.2010.01169

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Antioxidative Activities of Transgenic Yeast with Ferritin Gene from Wheat

ZHAO Yong-Liang1,2,CHEN Jing1,WANG Dan1,WANG Wei-Guo1, LI Yun-Hui1   

  1. 1Department of Biology Engineering,Henan University of Technology,Zhengzhou,45001,China;2The National Key Facility for Crop Gene Resources and Genetic Improvement,Key Laboratory of Crop Germplasm and Biotechnology,Ministry of Agriculture,Institute of Crop Sciences,Chinese Academy of Agricultural Sciencses,Beijing 100081.China
  • Received:2009-12-17 Revised:2010-03-19 Online:2010-07-12 Published:2010-04-28

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Abstract: Ferritin, a key protein to store iron, is widely distributed in animal, plant, and microbe. Ferritin can regulate the balance in iron metabolism and decrease the oxidative damage caused by ferrous ion. It is generally recognized that the antioxidant activity of ferritin is mainly caused by integrating ferrous ion among cells, and thereby decreasing the generation of Fenton reaction. In addition to the antioxidant pathway against hydrogen peroxide (H2O2), less information is available on other antioxidative pathways and mechanism of ferritin. In this study, a ferritin gene was cloned from wheat (Triticum aestivum L.), and its expression vehicle of eukaryotic cell was constructed and transformed into yeast (Saccharomyces cerevisiae). Compared with the control yeasts, the transgenic yeasts were able to scavenge H2O2,O2?and ·OH effectively. The half lethal density (LD50) of transgenic yeasts induced by the three types of active oxygen (·OH, O2?and H2O2) was 0.40, 1.00, and 36.91 mmol L-1, which increasedby37.93%, 47.06%, and 77.03% compared with the control respectively. The abilities for eliminating ·OH, O2?, and H2O2 of the trsnsgenic yeast per unit concentiation were quantitated with cubic polynomial, logarithmic, and negative exponential equations based on yeast concentration respectively. These results demonstrated that expressed products of wheat ferritin gene in eukaryotic orgamism can effectivly in eliminate various types of active oxygen with different and complex mechanisms.

Key words: Wheat, Ferritin, Trangenic yeast, Antioxdative activity

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