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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2116-2123.doi: 10.3724/SP.J.1006.2010.02116

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line M8003-5

XU Zhong-Qing1,ZHANG Shu-Ying1,WANG Rui1,WANG Wen-Li1,ZHOU Xin-Li1,Chen Jie2,JING Jin-Xue1,*   

  1. 1 College of Plant Protection / Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China; 2Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China
  • Received:2010-03-16 Revised:2010-05-21 Online:2010-12-12 Published:2010-08-30
  • Contact: JING Jin-Xue,E-mail:jingjinxue@163.com,Tel:029-87092434

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Abstract: Strip rust, caused by Puccinia striiformis West. f. sp. tritici (Pst) is a worldwide disease in wheat (Triticum aestivum L.) production. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Wheat genetic resources carrying novel resistance genes always receive great attentions and the utilization study generally initiates from the identification and genetic analysis of the resistance genes. Wheat line M8003-5 is selected from the hybrid progenies of the common wheat variety Chinese Spring and Secale cereale L., which exhibits not only high resistance to Pst but also high yield, early maturity, and resistance to drought. To map the Pst resistance gene(s) in M8003-5, a segregation population was constructed by crossing M8003-5 and a highly susceptible variety Mingxian 169. Seven prevalent Pst races in China were inoculated in greenhouse at seedling stage to evaluate the infection type of individuals from the F1, F2, and F3 generations. The results showed that M8003-5 was resistant to all races, whereas Mingxian 169 was highly susceptible to all inoculates. Genetic analysis indicated that the resistance of M8003-5 against race Su11-4 was conferred by a dominant gene, which was tentatively designated YrM8003. This gene was linked to simple sequence repeat (SSR) markers Xbarc5, Xwmc463, Xwmc405, Xbarc126, Xgwm295, Xgwm44, Xwmc702, Xwmc438, Xwmc121, Xgwm111, and Xbarc128, which were all located on chromosome arm 7DS. The closest flanking makers were Xwmc702 and Xwmc438 with genetic distance of 3.5cM and 4.3 cM, respectively. Gene YrM8003 differs from any other Pst resistance genes on 7DS, and is probably a novel gene. This gene is primarily inferred to originate fromS. cereale based on analyses on pedigree and molecular markers data. Forty-three wheat cultivars from Huang-Huai Winter Wheat Region were also tested with YrM8003 markers Xwmc702 and Xwmc438, and 20% cultivars were amplified with the target bands. These cultivars require further tests to validate the presence of YrM8003.

Key words: M8003-5, Stripe rust resistance, Genetic analysis, SSR marker, Molecular marker-assisted selection

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