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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2116-2123.doi: 10.3724/SP.J.1006.2010.02116

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line M8003-5

XU Zhong-Qing1,ZHANG Shu-Ying1,WANG Rui1,WANG Wen-Li1,ZHOU Xin-Li1,Chen Jie2,JING Jin-Xue1,*   

  1. 1 College of Plant Protection / Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China; 2Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China
  • Received:2010-03-16 Revised:2010-05-21 Online:2010-12-12 Published:2010-08-30
  • Contact: JING Jin-Xue,E-mail:jingjinxue@163.com,Tel:029-87092434

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Abstract: Strip rust, caused by Puccinia striiformis West. f. sp. tritici (Pst) is a worldwide disease in wheat (Triticum aestivum L.) production. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Wheat genetic resources carrying novel resistance genes always receive great attentions and the utilization study generally initiates from the identification and genetic analysis of the resistance genes. Wheat line M8003-5 is selected from the hybrid progenies of the common wheat variety Chinese Spring and Secale cereale L., which exhibits not only high resistance to Pst but also high yield, early maturity, and resistance to drought. To map the Pst resistance gene(s) in M8003-5, a segregation population was constructed by crossing M8003-5 and a highly susceptible variety Mingxian 169. Seven prevalent Pst races in China were inoculated in greenhouse at seedling stage to evaluate the infection type of individuals from the F1, F2, and F3 generations. The results showed that M8003-5 was resistant to all races, whereas Mingxian 169 was highly susceptible to all inoculates. Genetic analysis indicated that the resistance of M8003-5 against race Su11-4 was conferred by a dominant gene, which was tentatively designated YrM8003. This gene was linked to simple sequence repeat (SSR) markers Xbarc5, Xwmc463, Xwmc405, Xbarc126, Xgwm295, Xgwm44, Xwmc702, Xwmc438, Xwmc121, Xgwm111, and Xbarc128, which were all located on chromosome arm 7DS. The closest flanking makers were Xwmc702 and Xwmc438 with genetic distance of 3.5cM and 4.3 cM, respectively. Gene YrM8003 differs from any other Pst resistance genes on 7DS, and is probably a novel gene. This gene is primarily inferred to originate fromS. cereale based on analyses on pedigree and molecular markers data. Forty-three wheat cultivars from Huang-Huai Winter Wheat Region were also tested with YrM8003 markers Xwmc702 and Xwmc438, and 20% cultivars were amplified with the target bands. These cultivars require further tests to validate the presence of YrM8003.

Key words: M8003-5, Stripe rust resistance, Genetic analysis, SSR marker, Molecular marker-assisted selection

[1]Li Z-Q(李振岐), Zeng S-M(曾士迈). Wheat Rust in China (中国小麦条锈). Beijing: China Agriculture Press, 2002. pp 41–50 (in Chinese)
[2]Wan A-M(万安民), Niu Y-C(牛永春), Wu L-R(吴立人), Yuan W-H(袁文焕), Li G-B(李高宝), Jia Q-Z(贾秋珍), Jin S-L(金社林), Yang J-X(杨家秀), Li X-F(李艳芳), Bi Y-Q(毕云清). Physiology specialization of stripe rust of wheat in China during 1991–1996. Acta Phytopathol Sin (植物病理学报), 1999, 29(1): 15–20 (in Chinese with English abstract)
[3]Yang L-M(杨作民), Tang B-R(唐伯让), Shen K-Q(沈克全), Xia X-C(夏先春). A strategic problem in wheat resistance breeding-building and utilization of sources of second-line resistance against rust and mild in China. Acta Agron Sin (作物学报), 1994, 12(7): 385–394 (in Chinese with English abstract)
[4]Wan A-M(万安民), Wu L- R(吴立人), Jia Q-Z(贾秋珍), Jin S-L(金社林), Wang B-T(王保通), Yao G(姚革), Yang J-X(杨家秀), Yuan Z-Y(原宗英), Bi Y-Q(毕云清). Pathogenic changes of stripe rust fungus of wheat in China during 1997–2001. Acta Phytopathol Sin (植物病理学报), 2003, 33(3): 261–266 (in Chinese with English abstract)
[5]Yang Z-M(杨作民), Xie C-J(解超杰), Sun Q-X(孙其信). Situation of the sources of stripe rust resistance of wheat in the post CY32 era in China. Acta Agron Sin (作物学报), 2003, 29(2): 161–168 (in Chinese with English abstract)
[6]He J-B(何家泌). Inheritance of wheat rust resistance and its breeding. Sci Agric Sin (中国农业科学), 1980, 23(4): 106–109 (in Chinese with English abstract)
[7]Wu J-H(吴金华), Ji W-Q(吉万全), Wang C-Y(王长有), Li F-Z(李凤珍). Identification of wheat-rye addition lines resistant to powdery mildew. Chin Agric Sci Bull (中国农学通报), 2005, 10(10): 279–281 (in Chinese with English abstract)
[8]Li Z-S(李振声), Rong S(容珊), Zhong G-C(钟冠昌). Distant Hybridization in Wheat (小麦远源杂交). Beijing: Science Press, 1985. pp 84–129 (in Chinese)
[9]Friebe B, Jiaag J, Raupp W J. Characterization of wheat-alien translocations conferring resistance to diseases and pests: Current status. Euphytica, 1996, 91: 58–87
[10]Smith E L, Sebesta E E. The transfer of greenbug resistance from rye to wheat. In: Proceedings of the 6th International Wheat Genetics Symposium, 1980. pp 79–90
[11]Wang L-S(王林生), Chen P-D(陈佩度). Inducement of alien translocation lines and its utility in crop breeding in Triticum aestivum. Bull Biol (生物学通报), 2007, 42(2): 9–11 (in Chinese with English abstract)
[12]Xue X-Z(薛秀庄). Wheat Chromosome Engineering and Breeding (小麦染色体工程和育种). Shijiazhuang: Heibei Science and Technology Press, 1989. pp 115–118 (in Chinese)
[13]Li Z-Q(李振岐), Shang H-S(商鸿生). Wheat Stripe Rust and Its Controlling Strategies (小麦条锈病防治策略). Shanghai: Shanghai Scientific and Technical Publishers, 1989. p 214 (in Chinese)
[14]Li Z-Q(李振岐), Zeng S-M(曾士迈). Wheat Rust in China (中国小麦条锈). Beijing: China Agriculture Press, 2002 (in Chinese)
[15]Liu X-K(刘孝坤). A preliminary study on the inheritance of resistance to stripe rust in wheat. Acta Phytophylacica Sin (植物保护学报), 1988, 15(1): 33–39 (in Chinese with English abstract)
[16]He J-B(何家泌). Inheritance of Resistance to Plant Disease (病害抗病遗传). Beijing: China Agriculture Press, 1994. pp 215–222 (in Chinese)
[17]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69–76
[18]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease resistance genes by bulked sergeant analysis: A rapid method to detect markers in specific genomic regions by using segregating segregation populations. In: Proceedings of the National Academy of Sciences of the United States of America, San Diego, USA. 1991, Vol. 88, pp 9828–9832
[19]Liu R-H(刘仁虎), Meng J-L(孟金陵). MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (遗传), 2003, 25(3): 317–321 (in Chinese with English abstract)
[20]Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L). Theor Appl Genet, 2004, 109: 1105–1114
[21]Cao Z-J(曹张军), Wang M-N(王美南), Jing J-X(井金学).The progress of resistance to stripe rust in wheat. J Triticeae Crops (麦类作物学报), 2001, 21(3): 80–83 (in Chinese with English abstract)
[22]Autrique J E. RFLP mapping of genes associated with different agronomic traits and disease resistance in wheat. In: Abstract of International Plant Gene III, San Diego, USA
[23]Nazari K, Wellings C R. Genetic analysis of seedling stripe rust resistance in the ustralian wheat cultivar ‘Batavia’. J Plant Genet Resour, 2002, 3(4): 102
[24]Zhang H-Q(张海泉), Jia J-Z(贾继增), Yang H(杨虹), Zhang B-S(张宝石). Genetic analysis and SSR mapping of stripe rust resistant genes from Aegilops tauschii. Heredity (遗传), 2008, 30(3): 491–494 (in Chinese with English abstract)
[25]Li G Q, Li Z F, Yang W Y. Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26. Theor Appl Genet, 2006, 112: 1434−1340
[26]Spielmeyer W , McIntosh R A,Kolmer J, Lagudah E S. Powdery mildew resistance and Lr34/Yr18 genes for durable resistance toleaf and stripe rust cosegregate at a locus on the short aim ofchromosome 7D of wheat. Theor Appl Genet,2005：731-735
[27]Ren Z-L(任志龙), Zhang H(张宏), Wang K-F(王康峰), Wang Y-J(王亚娟), Cai D-M(蔡东明), Ji W-Q(吉万全), Song Y-L(宋玉莲). Development of wheat germplasm with disease resistance-Yuanfeng 139. Chin Agric Sci Bull (中国农学通报), 2006, 22(7): 228−231 (in Chinese with English abstract)
[28]Ren Z-L(任志龙), Ji W-Q(吉万全), Zhang H(张宏). The high yield and resistant disease wheat line Shaan 139. China Seed Ind (中国种业). 2007, (5): 73 (in Chinese)
[29]Jing J-X(井金学), Xu Z-B(徐智斌), Wang D-B(王殿波), Wang M-A(王美南), Yao Q-Y(姚秋燕), Shang H-S(商鸿生), Li Z-Q(李振岐). Genetic analysis of gene conferring resistance tostripe rust in Xiaoyan 6. Sci Agric Sin (中国农业科学), 2007, 40(3): 499–504 (in Chinese with English abstract)
[30]Zhuang Q-S(庄巧生). Chinese Wheat Improvement and Pedigree Analysis (小麦发展和育种分析). Beijing: China Agriculture Press, 2003 (in Chinese)
[31]Jin S-B(金善宝). Wheat Varieties in China and Their Pedigrees (中国小麦品种及其系谱). Beijing: Agriculture Press, 1983 (in Chinese)
[32]Li L-Z(李兰真), Yang H-W(杨会武), Zhao H-J(赵会杰). Super high-yielding, disease resistant, cold tolerant and broad adaptation new wheat variety “Yu nong 949”. Bull Agric Scin Technol (农业科技通讯), 2006, (4): 67–71 (in Chinese)
[33]Wen H-J(温辉芹), Zhang L-S(张立生), Cheng T-L(程天灵), Li S-H(李生海). Breeding practice and experience of the new strong gluten wheat variety of national authorized Jintai 170. J Shanxi Agric Sci (山西农业科学). 2008, 36(9): 15–20 (in Chinese with English abstract)
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