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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2154-2161.doi: 10.3724/SP.J.1006.2010.02154


Effect of Cd2+ on Seedling Growth and Proteome in Tomato

CHEN Li,WANG Lian,WANG Zhen-Ying*,PENG Yong-Kang*   

  1. College of Life Sciences / Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University, Tianjin 300387, China
  • Received:2010-03-23 Revised:2010-07-04 Online:2010-12-12 Published:2010-08-31
  • Contact: WANG Zhen-Ying,E-mail:wzycell@yahoo.com.cn;PENG Yong-Kang,E-mail:pykcell@yahoo.com.cn

Abstract: Cadmium is one of the most serious heavy metal pollutions in agricultural soils in China. Three-day-old tomato seedlings were treated with 0.01–1.00 mmol L–1 Cd2+ for 72 h. The results showed that seedling growth was obviously inhibited and seedling height was decreased from 4.76±0.5 cm (in control) to 3.79±0.05 cm (in 0.01 mmol L–1 Cd2+ treatment, P<0.01) and 1.77±0.15 cm (in 0.03 mmol L–1 Cd2+ treatment, P<0.01). In addition, root length was also decreased from 6.07±0.04 cm (in control) to 4.77±0.58 cm (in 0.01 mmol L–1 Cd2+ treatment, P<0.01) and 3.65±0.66 cm (in 0.03 mmol L–1Cd2+ treatment, P<0.01). The chlorophyll contents decreased in the treatment with 0.1 mmol L–1 Cd2+. Ten protein spots in roots and twenty one protein spots in leaves were altered when the seedlings were treated with 0.05 mmol L–1 Cd2+. Total ten protein spots in roots were identified by MS/MS. Four new proteins were induced in roots, including spot 1: ribosomal protein L 20, spot 2: F-box /LRR repeat protein, spot 4: ribosomal protein small submit 4 and spot 5: CBL-interacting protein kinase. In the leaves, two protein spots disappeared and four new protein spots were induced, including spot 16, ABC transporter; spot 17, maturase-like protein; spot 1, chalcone synthase; spot 3, a hypothetical protein; spot 4, an unknown protein and spot 6, a predicated protein. These Cd2+ responsive proteins identified could be involved in protein biosynthesis, mRNA transcription regulation and protein transport. The above results showed that tomato is one of the highly sensitive crops to Cd2+ and could be used as a model to study the adaptation and tolerance mechanisms to heavy metals at physiological and biochemical levels.

Key words: Cd2+-stress, Proteome, Partial amino acid sequence, MALDI-TOF-MS, Tomato (Lycopersicon esculentum L.)

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