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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 381-388.doi: 10.3724/SP.J.1006.2011.00381

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Mapping QTLs for Seminal Root Architecture and Coleoptile Length in Wheat

LIU Xiu-Lin1,2,CHANG Xiao-Ping2,LI Run-Zhi1,JING Rui-Lian2,*   

  1. 1 Shanxi Agricultural University, Taigu 030801, Shanxi, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-08-18 Revised:2010-12-06 Online:2011-03-12 Published:2011-01-17
  • Contact: 景蕊莲, E-mail: jingrl@caas.com.cn

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Abstract: Root system is important for belowground nutrients acquisition, and is also an important part to respond to drought stress. The purpose of this study was to dissect the genetic basis of seminal root architecture and coleoptile length of wheat by mapping quantitative trait loci (QTLs) of target traits. A doubled haploid (DH) population with 150 lines derived from a cross between two common Chinese wheat varieties Hanxuan 10 and Lumai 14 was used as the plant materials. Gel-chamber was employed to evaluate seminal root architecture traits, including maximum root length (MRL), root number (RN), total root length (TRL), root diameter (RD), root angle (RA), ratio of root dry weight to shoot dry weight (RDW/SDW), and coleoptile length (CL) of seedlings. QTLs for these traits were detected using mixed-model-based composite interval mapping method. A total of 12 additive-effect QTLs and 7 pairs of additive × additive QTLs associated with the target traits were mapped on chromosomes 1A, 1B, 2B, 2D, 3B, 4A, 4D, 5A, 5B, 6A, 7A, and 7B. The phenotypic variation explained by individual additive-effect QTL varied from 5.64% to 12.37%. The additive effects ranged from 0.20 to 7.45. The phenotypic variation explained by each pair of epistatic QTLs varied from 8.70% to 15.90%. Two QTL clusters for seedling root traits were detected on chromosomes 3B and 7A. These results would be helpful to maker-assisted selection of seminal root architecture and coleoptile.

Key words: Wheat, Seminal root architecture, Coleoptile length, QTL, DH lines, Gel-chamber

[1]Ma Y-X(马元喜). The Root of Wheat (小麦的根). Beijing: China Agriculture Press, 1999. pp 1–20 (in Chinese)
[2]Manschadi A M, Hammer G L, Christopher J T, deVoil P. Genotypic variation in seedling root architectural traits and implications for drought adaptation in wheat (Triticum aestivum L.). Plant Soil, 2008, 303: 115–129
[3]Manschadi A M, Christopher J, de Voil P, Hammer G L. The role of root architectural traits in adaptation of wheat to water-limited environments. Funct Plant Biol, 2006, 33: 823–837
[4]Hurd E A. Growth of roots of seven varieties of spring wheat at high and low moisture levels. Agron J, 1968, 60: 201–205
[5]Miao G-Y(苗果园), Zhang Y-T(张云亭), Yin J(尹钧), Hou Y-S(侯跃生), Pan X-L(潘幸来). A study on the development of root system in winter wheat under unirrigated conditions in semi-arid loess plateau. Acta Agron Sin (作物学报), 1989, 19(2): 104–115 (in Chinese with English abstract)
[6]Liang Y-L(梁银丽), Chan P-Y(陈培元). Characteristic of Wheat Varieties in Arid Region (旱地小麦品种的特征特性). In: Shan L. Fundamentals of Physiology and Ecology in Arid Agriculture. Beijing: Science Press, 1998. pp 259–266 (in Chinese)
[7]Li L-H(李鲁华), Li S-Q(李世清), Zhai J-H(翟军海), Shi J-H(史俊海). Review of the relationship between wheat roots and water stress. Acta Bot Boreali-Occident Sin (西北植物学报), 2001, 21(1): 1–7 (in Chinese with English abstract)
[8]Chang X-P(昌小平), Wang H(王嬛), Yang L(杨莉). Changes of root activity and water state at seedling stage of winter wheat varieties with different drought-resistance under different water conditions. Plant Physio Commun (植物生理学通讯), 1996, 32(3): 178–182 (in Chinese with English abstract)
[9]Duan S-S(段舜山), Gu W-X(谷文祥), Zhang D-Y(张大勇), Li F-M(李凤民). Relationship between root system characteristics and drought resistance of wheat populations in semiarid region. Chin J Appl Ecol (应用生态学报), 1997, 8(2): 134–138 (in Chinese with English abstract)
[10]Dhanda S S, Sethi G S, Behl R K. Indices of drought tolerance in wheat genotypes at early stages of plant growth. J Agron Crop Sci, 2004, 190: 6–12
[11]Champoux M C, Wang G, Sarkarung S, Mackill D J, Toole T C O, Huang N, McCouch S R. Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet, 1995, 90: 969–981
[12]Ray J D, Yu L X, Mccouch S R, Mackill D J, Toole T C O, Huang N, MeCouch S R. Mapping quantitative trait loci associated with root penetration ability in rice (Oryza sativa L.). Theor Appl Genet, 1996, 92: 627–636
[13]Mu P(穆平), Li Z-C(李自超), Li C-P(李春平), Zhang H-L(张洪亮), Wu C-M(吴长明), Li C(李晨), Wang X-K(王象坤). QTL mapping and G×E interaction for root traits in a DH population from japonica upland and lowland rice cross under three ecosystems. Chin Sci Bull (科学通报), 2003, 48(20): 2162–2169 (in Chinese)
[14]Zhang Z-B(张正斌), Xu P(徐萍). Reviewed on wheat genome. Heredity (遗传), 2002, 24(3): 389–394 (in Chinese with English abstract)
[15]Zhou X-G(周晓果), Jing R-L(景蕊莲), Hao Z-F(郝转芳), Chang X-P(昌小平), Zhang Z-B(张正斌). Mapping QTL for seedling root traits in common wheat. Sci Agric Sin (中国农业科学), 2005, 38(10): 1951–1957 (in Chinese with English abstract)
[16]Li Z-K(李卓坤), Peng T(彭涛), Zhang W-D(张卫东), Xie Q-G(谢全刚), Tian J-C(田纪春). Analysis of QTLs for root traits at seedling stage using an “Immortalized F2” population of wheat. Acta Agron Sin (作物学报), 2010, 36(3): 442–448 (in Chinese with English abstract)
[17]Bengough A G, Gordon D C, Al-Menaie H, Ellis R P, Allan D, Keith R, Thomas W T B, Forster B P. Gel observation chamber for rapid screening of root traits in cereal seedlings. Plant & Soil, 2004, 262: 63–70
[18]Sanguineti M C, Li S, Maccaferri M, Corneti S, Rotondo F, Chiari T, Tuberosa R. Genetic dissection of seminal root architecture in elite durum wheat germplasm. Ann Appl Biol, 2007, 151: 291–305
[19]Jing R-L(景蕊莲), Chang X-P(昌小平), Jia J-Z (贾继增), Hu R-H (胡荣海). Establishing wheat doubled haploid population for genetic mapping by anther culture. Biotechnology (生物技术), 1999, 9(3): 4–8 (in Chinese with English abstract)
[20]Hao Z F, Chang X P, Guo X J, Jing R L, Li R Z, Jia J Z. QTL mapping for drought tolerance at stages of germination and seedling in wheat (Triticum aestivum L.) using a DH population. Sci Agric China, 2003, 2: 943–949
[21]Yang J, Zhu J. Predicting superior genotypes in multiple environments based on QTL effects. Theor Appl Genet, 2005, 110: 1268−1274
[22]McIntosh R A, Hart G E, Devos K M, Rogers W J. Catalogue of gene symbols for wheat. 1999. http://grain.jouy.inra.fr/ggpages/wgc
[23]Reyniers F N, Binh T. Screening with Phosphorus32 for rooting depth among varieties of rain-fed rice. Paper Presented in the Conference on Rice in Africa, IITA, lbadan, NIgeria, 1977
[24]Jing R-L(景蕊莲), Hu R-H(胡荣海), Zhu Z-H(朱志华), Chang X-P(昌小平). A study on heritabilities of seedling morphological traits and drought resistance in winter wheat cultivars of different genotype. Act Bot Boreal-Occident Sin (西北植物学报), 1997, 17(2): 152–157 (in Chinese with English abstract)
[25]Price A H, Steele K A, Moore B J, Jones R G W. Upland rice grown in soil-?lled chambers and exposed to contrasting water-de?cit regimes: II. Mapping quantitative trait loci for root morphology and distributing. Field Crops Res, 2002, 76: 25–43
[26]An D G, Su J Y, Liu Q Y, Zhu Y G, Tong Y P, Li J M, Jing R L, Li B, Li Z S. Mapping QTLs for nitrogen uptake in relation to the early growth of wheat (Triticum aestivum L.). Plant & Soil, 2006, 284: 73–84
[27]Xu J-L(徐建龙), Xue Q-Z(薛庆中), Luo L-J(罗利军), Li Z-K(黎志康). QTL dissection of panicle number per plant and spikelet number per panicle in rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2001, 28(8): 752–759 (in Chinese with English abstract)
[28]Zhang K-P(张坤普), Xu X-B(徐宪斌), Tian J-C(田纪春). QTL mapping for grain yield and spike related traits in common wheat. Acta Agron Sin (作物学报), 2009, 35(2): 270−278 (in Chinese with English abstract)
[29]Rebetzke G J, Bruce S E, Kirkegaard J A. Longer coleoptiles improve emergence through crop residues to increase seedling number and biomass in wheat (Triticum aestivum L.). Plant & Soil, 2005, 272: 87–100
[30]Botwright T L, Rebetzke G J, Condon A G, Richards R A. The effect of rat genotype and temperature on coleoptile growth and dry matter partitioning in young wheat seedlings. Aust J Plant Physiol, 2001, 15: 417–423
[31]Rebetzke G J, Ellis M H, Bonnett D G., Richards R A. Molecular mapping of genes for coleoptile growth in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2007, 114: 1173–1183
[32]Rebetzke G J, Appels R, Morrison A, Richards R A, McDonald G, Ellis M H, Spielmeyer W, Bonnett D G. Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L.). Aust J Agric Res, 2001, 52: 1221–1234
[33]Landjeva S, Neumann K, Lohwasser U, Börner A. Molecular mapping of genomic regions associated with wheat seedling growth under osmotic stress. Biol Plantarum, 2008, 52: 259–266
[34]Wu X S, Wang Z H, Chang X P, Jing R L. Genetic dissection of the developmental behaviours of plant height in wheat (Triticum aestivum L.) under diverse water regimes. J Exp Bot, 2010, 61: 2923–2937
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