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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 745-754.doi: 10.3724/SP.J.1006.2011.00745

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Improving the Resistance of Wuyunjing 8 to Rice Stripe Virus via Molecular Marker-Assisted Selection

ZHANG Hong-Gen1,XU Zuo-Peng1,LI Peng1,LI Bo1,LIU Chao1,ZHU Bang-Hui2,XU Jie-Fen2,NIU Zhong-Yi2,TANG Shu-Zhu1,*,LIANG Guo-Hua1,GU Ming-Hong1   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; 2 Wujin Agricultural Institute of Jiangsu Province, Changzhou 213175, China
  • Received:2010-10-09 Revised:2011-01-06 Online:2011-05-12 Published:2011-03-24
  • Contact: 汤述翥, E-mail: sztang@yzu.edu.cn

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Abstract: In this study, a molecular marker-assisted selection (MAS) strategy was used to improve Wuyunjing 8’s resistance to rice stripe virus. The Wuyunjing 8 with high yield and erect head selected from 25 late-maturing varieties was used as accepter, and the variety Kuifeng with resistance to rice stripe virus as the donor. Four STS (sequence-tagged site) primers STS11-31, STS11-7, STS11-19, and STS11-43 anchoring the resistance genes were used for assisted selection in the backcross progenies. To 2008, seventy lines in BC3F5 and one hundred and fifteen lines of BC4F4 with stable agronomic traits were obtained with the result of molecular analysis. By appraising agronomic traits，yield , quality and resistance in backcross progenies, teen lines from BC4F5 and two lines from BC3F6, which all carried disease-resistant genes and were similar to Wuyunjing 8 on the integrated traits, were selected. These lines retain the characteristics of high yield and good quality of Wuyunjing 8 and have been prominently improved in resistance to rice stripe virus.

Key words: Japonica, Rice stripe virus, Resistance, Improvement, Molecular marker-assisted selection

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