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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 1069-1076.doi: 10.3724/SP.J.1006.2011.01069

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Regulation of Nitrogen Level on Photosynthetic Energy Partitioning in Wheat Leaves under Elevated Atmospheric CO2 Concentration

ZHANG Xu-Cheng1,2,YU Xian-Feng1,WANG Hong-Li3,MA Yi-Fan1   

  1. 1 Key Laboratory of Northwest Crop Drought-Resistant Farming of Ministry of Agriculture / Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2 College of Resources and Environment, China Agricultural University, Beijing 100193, China; 3College of Agronomy, Gansu agricultural University, Lanzhou 730070, China
  • Received:2010-11-16 Revised:2011-03-28 Online:2011-06-12 Published:2011-04-12
  • Contact: E-mail: gszhangxuch@163.com, Tel: 0931-7612800

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Abstract: The objective of this study was to understand the regulatory mechanism of nitrogen (N) application on photosynthetic acclimation under elevated atmospheric CO2 concentration. The ambient atmospheric CO2 concentration was 400 μmol mol−1, and the elevated CO2 concentration was 760 μmol mol−1, which was simulated with Top Open Chambers. Wheat (Triticum aestivum L.) cultivar Ningchun 4 was grown in pots under both CO2 concentrations and treated with low (0 mg pure N per kilogram soil, N0) and high (200 mg pure N per kilogram soil, N200) N application levels. The photosynthetic electron transport rate was estimated using the response curve between photosynthetic rate (Pn) and intercellular CO2 concentration (Ci) and chlorophyll fluorescence parameters. The leaf nitrogen concentrations were also measured at jointing and heading stages. Compared to that under the ambient CO2 concentration, the leaf nitrogen concentration of wheat was decreased significantly under the elevated CO2 concentration, and the percentage of decrease in N200 treatment was 335.7% higher at heading than jointing stage. The values of maximal quantum yield under irradiance (Fv′/Fm′), actual PSII efficiency under irradiance (ΦPSII), photochemical fluorescence quenching (qP) were higher in N200 treatment than in N0 treatment; however, the value of non-photochemical fluorescence quenching (NPQ) was higher in N0 treatment than in N200 treatment. Under elevated atmospheric CO2 concentration, the electronic transport rate of photochemistry (Jc) and Rubisco carboxylation rates (Vc) were increased significantly, but the electronic transport rate of photorespiration (Jo) and Rubisco oxygenation rate (Vo) were decreased significantly. N application tended to promote the values of Jc, Jo, Vc, and Vo, especially for Jc and Vc, which had significant increase compared to those in N0 treatment. When the atmospheric CO2 concentration elevated from 400 μmol mol−1 to 760 μmol mol−1, the rations of Jo/Jc and Vo/Vc decreased significantly. Under the elevated atmospheric CO2 concentration, N application decreased Jo/Jc and Vo/Vc significantly at jointing stage, but increased Jo/Jc at heading stage and remained Vo/Vc with no significant difference. The leaf nitrogen concentration was positively correlated with Jc (P < 0.01), Jo, (P < 0.05) and Vo (P < 0.05), and the sensitivities of Jc, Jo, and Vo in response to leaf N concentration were decreased under elevated atmospheric CO2 concentration. The opening ratio of PSII reaction center was increased under elevated atmospheric CO2 concentration, and the non-photochemical energy dissipation was decreased simultaneously, resulting in more photosynthetic electron transported to photochemical process in wheat leaf. N application had a positively effect on photosynthetic function and changed its energy partitioning through increasing leaf N concentration. This might be one of the key reasons for photosynthesis acclimation of C3 plant to N sufficient application under elevated atmospheric CO2 concentration.

Key words: Elevated atmospheric CO2 concentration, Nitrogen application rate, Photosynthetic electron transport rate, Photosynthetic energy partition, Wheat

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