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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1339-1345.doi: 10.3724/SP.J.1006.2012.01339

• RESEARCH NOTES • Previous Articles    

Inheritance of Resistance to Powdery Mildew in 12 Wheat Cultivars

SONG Feng-Jing1,XIAO Ming-Gang1,HUANG Jiang1,2,3,WANG Xiao-Ming1,ZHU Zhen-Dong1,WU Xiao-Fei1,LI Hong-Jie1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 2 Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; 3 Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-12-30 Revised:2012-04-16 Online:2012-07-12 Published:2012-05-11
  • Contact: 李洪杰, E-mail: hongjie@caas.net.cn

Abstract: Wheat powdery mildew, caused by the biotrophic parasitic fungus Blumeria graminis (DC.) f. sp. tritici E.O. Speer. is one of the most epidemic diseases in many wheat (Triticum aestivum L.) producing regions in China and other parts of the world. The information on inheritance of resistance to powdery mildew in commercial wheat cultivars is important for controlling the disease and developing new resistant cultivars. In the present study, 17 isolates of B. graminis were used to postulate and analyze powdery mildew resistance genes in 12 wheat cultivars at seedling stage. Molecular detection was performed to disclose the presence of Pm2 and Pm8 for powdery mildew resistance. All of the 12 wheat cultivars were resistant to at least 11 tested isolates. Isolates E09, E20, and Bg2 were used to test F2 populations for analyzing inheritance of powdery mildew resistance in these wheat cultivars. The results demonstrated that single dominant gene(s) was responsible for powdery mildew resistance in these cultivars. The reactions to different isolates of B. graminis and analysis of amplification with Pm2-linked marker Xcfd81showed that Pm2 or its allele was most likely present in Liangxing 66. Using ω-secalin gene- and Glu-B1 gene-specific markers on chromosome arms 1RS and 1BS, respectively, the presence of T1BL·1RS translocation chromosome carrying Pm8 was detected in Shannong 20 and Zhengmai 9962. Since Pm8 was not effective against most isolates tested in this study, other unknown genes for powdery mildew resistance could be present in Shannong 20 and Zhengmai 9962 in addition to Pm8. The reaction patterns of Yanzhan 4110 and Tianmin 668 were identical. The remaining cultivars developed different patterns of reaction to the B. graminis isolates tested.

Key words: Wheat, Powdery mildew, Resistance gene, Inheritance

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