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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (02): 368-372.doi: 10.3724/SP.J.1006.2013.00368

• RESEARCH NOTES • Previous Articles     Next Articles

Overexpression of BvGLP1 in Transgenic Wheat Enhances Resistance to Common Root Rot

DANG Liang1,2,**,SU Zhen-Qi3,**,YE Xing-Guo2,XU Hui-Jun2,LI Zhao2,SHAO Yan-Jun1,*,ZHANG Zeng-Yan2,*   

  1. 1 College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Institute of Grain and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China
  • Received:2012-07-16 Revised:2012-10-09 Online:2013-02-12 Published:2012-12-11
  • Contact: 张增艳, E-mail: zhangzy@mail.caas.net.cn, Tel: 010-82108781 **同等贡献(Contributed equally to the work)

Abstract:

BvGLP1 is a kind of germin-like protein (GLP) from sugar beet. GLP catalyzes the oxidation of oxalic acid to produce hydrogen peroxide that induces plant defense response to pathogen and results in enhanced-resistance. The open-reading-frame sequenceof BvGLP1 was synthesized and used to construct a BvGLP1 expression vector pA20-RSS1P::BvGLP1. In the vector, the expression of BvGLP1 was controlled by rice sucrose synthase-1 promoter (RSS1P). BvGLP1 was introducedinto wheat variety Yangmai 18 through bombardment. The presence and expression of BvGLP1 in T0 to T3 transgenic wheat plants were characterized by PCR, RT-PCR, and QPCR analyses. The common root rot and sharp eyespot disease tests on BvGLP1 transgenic wheat plants following artificial inoculation with the pathogens revealed that the expression of BvGLP1 in five transgenic wheat lines significantly enhanced resistance to common root rot.

Key words: Germin-like protein, BvGLP1, Transgenic wheat, Wheat common root rot, Resistance

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